Reviewed, UniProtKB/Swiss-Prot B5YQ33 (ASTD_ECO5E)

Names and origin

Protein names

Recommended name:
    N-succinylglutamate 5-semialdehyde dehydrogenase
    EC=1.2.1.71
Alternative name(s):
    Succinylglutamic semialdehyde dehydrogenase
      Short name=SGSD

Gene names

Name:	astD
Ordered Locus Names:	ECH74115_2464

Organism

Escherichia coli O157:H7 (strain EC4115 / EHEC) [Complete proteome] [HAMAP]

Taxonomic identifier

444450 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	492 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the NAD-dependent reduction of succinylglutamate semialdehyde into succinylglutamate By similarity.
Catalytic activity	N-succinyl-L-glutamate 5-semialdehyde + NAD+ + H2O = N-succinyl-L-glutamate + NADH. HAMAP MF_01174
Pathway	Amino-acid degradation; L-arginine degradation via AST pathway; L-glutamate and succinate from L-arginine: step 4/5. HAMAP MF_01174
Sequence similarities	Belongs to the aldehyde dehydrogenase family. AstD subfamily.

Ontologies

Keywords
Biological process	Arginine metabolism
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine catabolic process to glutamate Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	succinylglutamate-semialdehyde dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 492	492	N-succinylglutamate 5-semialdehyde dehydrogenase HAMAP MF_01174		PRO_1000138042
Regions
	Nucleotide binding	220 – 225	6	NAD By similarity
Sites
	Active site	243	1	By similarity
	Active site	277	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	B5YQ33-1 [UniParc]. Last modified November 25, 2008. Version 1. Checksum: 147D74C2088D9221	FASTA	492	52,985
	10 20 30 40 50 60 MTLWINGDWI TGQGASRVKR NPVSGEVLWQ GNDADAAQVG QACRAARAAF PRWARLSFAE 70 80 90 100 110 120 RQAVVERFAG LLERNKGELT AIIARETGKP RWEAATELTA MINKIAISIK AYHVRTGEQR 130 140 150 160 170 180 SEMPDGAASL RHRPHGVLAV FGPYNFPGHL PNGHIVPALL AGNTIIFKPS ELTPWSGEAV 190 200 210 220 230 240 MRLWQQAGLP PGVLNLVQGG CETGQALSAL EDLDGLLFTG SANTGYQLHR QLSGQPEKIL 250 260 270 280 290 300 ALEMGGNNPL IIDEVADIDA AVHLTIQSAF VTAGQRCTCA RRVLLKSGAQ GDAFLARLVA 310 320 330 340 350 360 VSQRLTPGNW DDEPQPFIGG LISEQAAQQV FTAWQQLEAM GGRTLLAPRL LQAGTSLLTP 370 380 390 400 410 420 GIIEMTGVAG VPDEEVFGPL LRVWRYDSFE EAIRMANNTR FGLSCGLVSP EREKFDQLLL 430 440 450 460 470 480 EARAGIVNWN KPLTGAASTA PFGGIGASGN HRPSAWYAAD YCAWPMASLE SDSLTLPATL 490 NPGLDFSDEV VR

References

[1]	"Complete genome sequence of Escherichia coli O157:H7 str. EC4115." Eppinger M., Sebastian Y., Ravel J. Submitted (SEP-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP001164 Genomic DNA. Translation: ACI37962.1.
RefSeq	YP_002270815.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	6968544.
GenomeReviews	Gene locus ECH74115_2464 in contig CP001164_GR.
KEGG	ecf:ECH74115_2464.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	KAYHART.
Family and domain databases
HAMAP	MF_01174. [Tree]
InterPro	IPR016160. Ald_DH_CS. IPR016162. Ald_DH_N. IPR015590. Aldehyde_DH. IPR017649. SuccinylGlu_semiald_DH_AstD. [Graphical view]
Gene3D	G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 1 hit.
PANTHER	PTHR11699. Aldehyde_dehyd. 1 hit.
Pfam	PF00171. Aldedh. 1 hit. [Graphical view]
TIGRFAMs	TIGR03240. arg_catab_astD. 1 hit.
PROSITE	PS00070. ALDEHYDE_DEHYDR_CYS. 1 hit. PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ASTD_ECO5E

Accession

Primary (citable) accession number: B5YQ33

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	November 25, 2008
Last modified:	November 3, 2009
This is version 12 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)