Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase

Gene

miaB

Organism
Thermodesulfovibrio yellowstonii (strain ATCC 51303 / DSM 11347 / YP87)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the methylthiolation of N6-(dimethylallyl)adenosine (i6A), leading to the formation of 2-methylthio-N6-(dimethylallyl)adenosine (ms2i6A) at position 37 in tRNAs that read codons beginning with uridine.UniRule annotation

Catalytic activityi

N6-dimethylallyladenine(37) in tRNA + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine + reduced electron acceptor = 2-methylthio-N6-dimethylallyladenine(37) in tRNA + S-adenosyl-L-homocysteine + (sulfur carrier) + L-methionine + 5'-deoxyadenosine + electron acceptor.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 2 [4Fe-4S] clusters. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi13Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi49Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi83Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi154Iron-sulfur (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi158Iron-sulfur (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi161Iron-sulfur (4Fe-4S-S-AdoMet)UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processtRNA processing
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA-2-methylthio-N(6)-dimethylallyladenosine synthaseUniRule annotation (EC:2.8.4.3UniRule annotation)
Alternative name(s):
(Dimethylallyl)adenosine tRNA methylthiotransferase MiaBUniRule annotation
tRNA-i(6)A37 methylthiotransferaseUniRule annotation
Gene namesi
Name:miaBUniRule annotation
Ordered Locus Names:THEYE_A1049
OrganismiThermodesulfovibrio yellowstonii (strain ATCC 51303 / DSM 11347 / YP87)
Taxonomic identifieri289376 [NCBI]
Taxonomic lineageiBacteriaNitrospiraeNitrospiralesNitrospiraceaeThermodesulfovibrio
Proteomesi
  • UP000000718 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003746101 – 431tRNA-2-methylthio-N(6)-dimethylallyladenosine synthaseAdd BLAST431

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi289376.THEYE_A1049.

Structurei

3D structure databases

ProteinModelPortaliB5YKW2.
SMRiB5YKW2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini4 – 120MTTase N-terminalUniRule annotationAdd BLAST117
Domaini370 – 430TRAMUniRule annotationAdd BLAST61

Sequence similaritiesi

Belongs to the methylthiotransferase family. MiaB subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CIW. Bacteria.
COG0621. LUCA.
HOGENOMiHOG000224767.
InParanoidiB5YKW2.
KOiK06168.
OMAiKVCEHFH.
OrthoDBiPOG091H00LG.

Family and domain databases

Gene3Di3.80.30.20. 1 hit.
HAMAPiMF_01864. tRNA_metthiotr_MiaB. 1 hit.
InterProiView protein in InterPro
IPR006638. Elp3/MiaB/NifB.
IPR005839. Methylthiotransferase.
IPR020612. Methylthiotransferase_CS.
IPR013848. Methylthiotransferase_N.
IPR006463. MiaB_methiolase.
IPR007197. rSAM.
IPR023404. rSAM_horseshoe.
IPR002792. TRAM_dom.
PfamiView protein in Pfam
PF04055. Radical_SAM. 1 hit.
PF01938. TRAM. 1 hit.
PF00919. UPF0004. 1 hit.
SFLDiSFLDF00273. (dimethylallyl)adenosine_tRNA_. 1 hit.
SFLDG01061. methylthiotransferase. 1 hit.
SFLDS00029. Radical_SAM. 1 hit.
SMARTiView protein in SMART
SM00729. Elp3. 1 hit.
TIGRFAMsiTIGR00089. TIGR00089. 1 hit.
PROSITEiView protein in PROSITE
PS51449. MTTASE_N. 1 hit.
PS01278. MTTASE_RADICAL. 1 hit.
PS50926. TRAM. 1 hit.

Sequencei

Sequence statusi: Complete.

B5YKW2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKGRAVYIKT FGCQMNEHDS ERMLGILGTK GFIEVDEPKK ADIVIFNTCA
60 70 80 90 100
IRHKAEQKFF SSLGRVKHLK KKNPQLKIIV AGCSAQLQGE KLLNKLPYID
110 120 130 140 150
YIIGPDNLHV IENIIENQVS HRIFTDENPE VANINLPVKR KDCVKAWVNI
160 170 180 190 200
IYGCNNYCTY CVVPYTRGKE RSRPVDDIIK EISLLAEQGY KEVTLLGQNV
210 220 230 240 250
NSYKDGNTNF PLLLEKVEKI EGIKRIRFIT SHPKDLSKEL VDVMKDYKKI
260 270 280 290 300
CEHIHLPLQA GSNKILKLMN RKYTYEEYFE KICWLREAIP DIAITSDIIV
310 320 330 340 350
GFPQEQHEDF EKTINALKEI RFDGIFAFKF SPRLGTAAAK LDGHISEEVK
360 370 380 390 400
AARLIEVLKL QDEITERKNK RLEGKIQEVL VEGKDEEGFT TGKTRTNKVV
410 420 430
KIYSDIKAGE IVNVKIAKTH RHSLEGDIIS T
Length:431
Mass (Da):49,301
Last modified:November 25, 2008 - v1
Checksum:iF4420B3EC8F60FAC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001147 Genomic DNA. Translation: ACI21164.1.
RefSeqiWP_012545886.1. NC_011296.1.
YP_002248877.1. NC_011296.1.

Genome annotation databases

EnsemblBacteriaiACI21164; ACI21164; THEYE_A1049.
GeneIDi6942344.
KEGGitye:THEYE_A1049.
PATRICifig|289376.4.peg.1029.

Entry informationi

Entry nameiMIAB_THEYD
AccessioniPrimary (citable) accession number: B5YKW2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: November 25, 2008
Last modified: June 7, 2017
This is version 57 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families