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Protein

tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase

Gene

miaB

Organism
Thermodesulfovibrio yellowstonii (strain ATCC 51303 / DSM 11347 / YP87)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the methylthiolation of N6-(dimethylallyl)adenosine (i6A), leading to the formation of 2-methylthio-N6-(dimethylallyl)adenosine (ms2i6A) at position 37 in tRNAs that read codons beginning with uridine.UniRule annotation

Catalytic activityi

N6-dimethylallyladenine(37) in tRNA + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine + reduced electron acceptor = 2-methylthio-N6-dimethylallyladenine(37) in tRNA + S-adenosyl-L-homocysteine + (sulfur carrier) + L-methionine + 5'-deoxyadenosine + electron acceptor.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 2 [4Fe-4S] clusters. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi13Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi49Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi83Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi154Iron-sulfur (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi158Iron-sulfur (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi161Iron-sulfur (4Fe-4S-S-AdoMet)UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processtRNA processing
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciTYEL289376:G1GCQ-1048-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA-2-methylthio-N(6)-dimethylallyladenosine synthaseUniRule annotation (EC:2.8.4.3UniRule annotation)
Alternative name(s):
(Dimethylallyl)adenosine tRNA methylthiotransferase MiaBUniRule annotation
tRNA-i(6)A37 methylthiotransferaseUniRule annotation
Gene namesi
Name:miaBUniRule annotation
Ordered Locus Names:THEYE_A1049
OrganismiThermodesulfovibrio yellowstonii (strain ATCC 51303 / DSM 11347 / YP87)
Taxonomic identifieri289376 [NCBI]
Taxonomic lineageiBacteriaNitrospiraeNitrospiralesNitrospiraceaeThermodesulfovibrio
Proteomesi
  • UP000000718 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003746101 – 431tRNA-2-methylthio-N(6)-dimethylallyladenosine synthaseAdd BLAST431

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi289376.THEYE_A1049

Structurei

3D structure databases

ProteinModelPortaliB5YKW2
SMRiB5YKW2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini4 – 120MTTase N-terminalUniRule annotationAdd BLAST117
Domaini370 – 430TRAMUniRule annotationAdd BLAST61

Sequence similaritiesi

Belongs to the methylthiotransferase family. MiaB subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CIW Bacteria
COG0621 LUCA
HOGENOMiHOG000224767
InParanoidiB5YKW2
KOiK06168
OMAiKVCEHFH
OrthoDBiPOG091H00LG

Family and domain databases

Gene3Di3.40.50.12160, 1 hit
3.80.30.20, 1 hit
HAMAPiMF_01864 tRNA_metthiotr_MiaB, 1 hit
InterProiView protein in InterPro
IPR006638 Elp3/MiaB/NifB
IPR005839 Methylthiotransferase
IPR020612 Methylthiotransferase_CS
IPR013848 Methylthiotransferase_N
IPR038135 Methylthiotransferase_N_sf
IPR006463 MiaB_methiolase
IPR007197 rSAM
IPR023404 rSAM_horseshoe
IPR002792 TRAM_dom
PfamiView protein in Pfam
PF04055 Radical_SAM, 1 hit
PF01938 TRAM, 1 hit
PF00919 UPF0004, 1 hit
SFLDiSFLDF00273 (dimethylallyl)adenosine_tRNA_, 1 hit
SFLDG01061 methylthiotransferase, 1 hit
SFLDS00029 Radical_SAM, 1 hit
SMARTiView protein in SMART
SM00729 Elp3, 1 hit
TIGRFAMsiTIGR00089 TIGR00089, 1 hit
PROSITEiView protein in PROSITE
PS51449 MTTASE_N, 1 hit
PS01278 MTTASE_RADICAL, 1 hit
PS50926 TRAM, 1 hit

Sequencei

Sequence statusi: Complete.

B5YKW2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKGRAVYIKT FGCQMNEHDS ERMLGILGTK GFIEVDEPKK ADIVIFNTCA
60 70 80 90 100
IRHKAEQKFF SSLGRVKHLK KKNPQLKIIV AGCSAQLQGE KLLNKLPYID
110 120 130 140 150
YIIGPDNLHV IENIIENQVS HRIFTDENPE VANINLPVKR KDCVKAWVNI
160 170 180 190 200
IYGCNNYCTY CVVPYTRGKE RSRPVDDIIK EISLLAEQGY KEVTLLGQNV
210 220 230 240 250
NSYKDGNTNF PLLLEKVEKI EGIKRIRFIT SHPKDLSKEL VDVMKDYKKI
260 270 280 290 300
CEHIHLPLQA GSNKILKLMN RKYTYEEYFE KICWLREAIP DIAITSDIIV
310 320 330 340 350
GFPQEQHEDF EKTINALKEI RFDGIFAFKF SPRLGTAAAK LDGHISEEVK
360 370 380 390 400
AARLIEVLKL QDEITERKNK RLEGKIQEVL VEGKDEEGFT TGKTRTNKVV
410 420 430
KIYSDIKAGE IVNVKIAKTH RHSLEGDIIS T
Length:431
Mass (Da):49,301
Last modified:November 25, 2008 - v1
Checksum:iF4420B3EC8F60FAC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001147 Genomic DNA Translation: ACI21164.1
RefSeqiWP_012545886.1, NC_011296.1
YP_002248877.1, NC_011296.1

Genome annotation databases

EnsemblBacteriaiACI21164; ACI21164; THEYE_A1049
GeneIDi6942344
KEGGitye:THEYE_A1049
PATRICifig|289376.4.peg.1029

Similar proteinsi

Entry informationi

Entry nameiMIAB_THEYD
AccessioniPrimary (citable) accession number: B5YKW2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: November 25, 2008
Last modified: March 28, 2018
This is version 59 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health