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Protein

Probable S-methyl-5'-thioinosine phosphorylase

Gene

THEYE_A0977

Organism
Thermodesulfovibrio yellowstonii (strain ATCC 51303 / DSM 11347 / YP87)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the reversible phosphorylation of S-methyl-5'-thioinosine (MTI) to hypoxanthine and 5-methylthioribose-1-phosphate. Involved in the breakdown of S-methyl-5'-thioadenosine (MTA), a major by-product of polyamine biosynthesis. Catabolism of (MTA) occurs via deamination to MTI and phosphorolysis to hypoxanthine.UniRule annotation

Miscellaneous

Although this enzyme belongs to the family of MTA phosphorylases based on sequence homology, it has been shown that conserved amino acid substitutions in the substrate binding pocket convert the substrate specificity of this enzyme from 6-aminopurines to 6-oxopurines.UniRule annotation

Catalytic activityi

S-methyl-5'-thioinosine + phosphate = hypoxanthine + S-methyl-5-thio-alpha-D-ribose 1-phosphate.UniRule annotation

Pathwayi: purine nucleoside salvage

This protein is involved in the pathway purine nucleoside salvage, which is part of Purine metabolism.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway purine nucleoside salvage and in Purine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei15PhosphateUniRule annotation1
Sitei169Important for substrate specificityUniRule annotation1
Binding sitei187Substrate; via amide nitrogenUniRule annotation1
Binding sitei188PhosphateUniRule annotation1
Sitei223Important for substrate specificityUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosyltransferase, Transferase
Biological processPurine salvage

Enzyme and pathway databases

BioCyciTYEL289376:GH9L-976-MONOMER.
UniPathwayiUPA00606.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable S-methyl-5'-thioinosine phosphorylaseUniRule annotation (EC:2.4.2.44UniRule annotation)
Alternative name(s):
5'-methylthioinosine phosphorylaseUniRule annotation
Short name:
MTI phosphorylaseUniRule annotation
Short name:
MTIPUniRule annotation
Gene namesi
Ordered Locus Names:THEYE_A0977
OrganismiThermodesulfovibrio yellowstonii (strain ATCC 51303 / DSM 11347 / YP87)
Taxonomic identifieri289376 [NCBI]
Taxonomic lineageiBacteriaNitrospiraeNitrospiralesNitrospiraceaeThermodesulfovibrio
Proteomesi
  • UP000000718 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004151391 – 265Probable S-methyl-5'-thioinosine phosphorylaseAdd BLAST265

Interactioni

Subunit structurei

Homotrimer.UniRule annotation

Protein-protein interaction databases

STRINGi289376.THEYE_A0977.

Structurei

3D structure databases

ProteinModelPortaliB5YKP5.
SMRiB5YKP5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni55 – 56Phosphate bindingUniRule annotation2
Regioni211 – 213Substrate bindingUniRule annotation3

Sequence similaritiesi

Belongs to the PNP/MTAP phosphorylase family. MTAP subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4107RHB. Bacteria.
COG0005. LUCA.
HOGENOMiHOG000228987.
InParanoidiB5YKP5.
KOiK00772.
OMAiNLYRSWG.
OrthoDBiPOG091H0228.

Family and domain databases

HAMAPiMF_01963. MTAP. 1 hit.
InterProiView protein in InterPro
IPR010044. MTAP.
IPR000845. Nucleoside_phosphorylase_d.
PANTHERiPTHR42679. PTHR42679. 1 hit.
PfamiView protein in Pfam
PF01048. PNP_UDP_1. 1 hit.
SUPFAMiSSF53167. SSF53167. 1 hit.
TIGRFAMsiTIGR01694. MTAP. 1 hit.

Sequencei

Sequence statusi: Complete.

B5YKP5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHNKQQVKIG IIGGSGLSES EAKKEIITIK TPYGEPSCPY EIEKIDDIEV
60 70 80 90 100
LFLRRHGQKH SIPPHKVNYR ANIYGFKNFG IERIFGVFAT GSLTENIPPG
110 120 130 140 150
SIVIPNQIID FTQGMRANTF YEEKKVVHID FTEPFCSEIR HYLLETARKI
160 170 180 190 200
GINVISHATY ICVNGPRLET AAEIKFFKNI GADIIGMTIM PEASLAREVE
210 220 230 240 250
ICYAAVAVVA NYAAGISKFP LTVKEVIETM EDSLDAVGFL IKETIKKLPE
260
ERKCLCKHAL KNASF
Length:265
Mass (Da):29,620
Last modified:November 25, 2008 - v1
Checksum:iB109DB0BD2497606
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001147 Genomic DNA. Translation: ACI21697.1.
RefSeqiWP_012546405.1. NC_011296.1.
YP_002248810.1. NC_011296.1.

Genome annotation databases

EnsemblBacteriaiACI21697; ACI21697; THEYE_A0977.
GeneIDi6941541.
KEGGitye:THEYE_A0977.
PATRICifig|289376.4.peg.962.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiMTIP_THEYD
AccessioniPrimary (citable) accession number: B5YKP5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 25, 2012
Last sequence update: November 25, 2008
Last modified: June 7, 2017
This is version 48 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families