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Protein

Probable S-methyl-5'-thioinosine phosphorylase

Gene

THEYE_A0977

Organism
Thermodesulfovibrio yellowstonii (strain ATCC 51303 / DSM 11347 / YP87)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the reversible phosphorylation of S-methyl-5'-thioinosine (MTI) to hypoxanthine and 5-methylthioribose-1-phosphate. Involved in the breakdown of S-methyl-5'-thioadenosine (MTA), a major by-product of polyamine biosynthesis. Catabolism of (MTA) occurs via deamination to MTI and phosphorolysis to hypoxanthine.UniRule annotation

Catalytic activityi

S-methyl-5'-thioinosine + phosphate = hypoxanthine + S-methyl-5-thio-alpha-D-ribose 1-phosphate.UniRule annotation

Pathwayi: purine nucleoside salvage

This protein is involved in the pathway purine nucleoside salvage, which is part of Purine metabolism.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway purine nucleoside salvage and in Purine metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei15 – 151PhosphateUniRule annotation
Sitei169 – 1691Important for substrate specificityUniRule annotation
Binding sitei187 – 1871Substrate; via amide nitrogenUniRule annotation
Binding sitei188 – 1881PhosphateUniRule annotation
Sitei223 – 2231Important for substrate specificityUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Purine salvage

Enzyme and pathway databases

BioCyciTYEL289376:GH9L-973-MONOMER.
UniPathwayiUPA00606.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable S-methyl-5'-thioinosine phosphorylaseUniRule annotation (EC:2.4.2.44UniRule annotation)
Alternative name(s):
5'-methylthioinosine phosphorylaseUniRule annotation
Short name:
MTI phosphorylaseUniRule annotation
Short name:
MTIPUniRule annotation
Gene namesi
Ordered Locus Names:THEYE_A0977
OrganismiThermodesulfovibrio yellowstonii (strain ATCC 51303 / DSM 11347 / YP87)
Taxonomic identifieri289376 [NCBI]
Taxonomic lineageiBacteriaNitrospiraeNitrospiralesNitrospiraceaeThermodesulfovibrio
Proteomesi
  • UP000000718 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 265265Probable S-methyl-5'-thioinosine phosphorylasePRO_0000415139Add
BLAST

Interactioni

Subunit structurei

Homotrimer.UniRule annotation

Protein-protein interaction databases

STRINGi289376.THEYE_A0977.

Structurei

3D structure databases

ProteinModelPortaliB5YKP5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni55 – 562Phosphate bindingUniRule annotation
Regioni211 – 2133Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the PNP/MTAP phosphorylase family. MTAP subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4107RHB. Bacteria.
COG0005. LUCA.
HOGENOMiHOG000228987.
InParanoidiB5YKP5.
KOiK00772.
OMAiGCDIVGM.
OrthoDBiPOG091H0228.

Family and domain databases

Gene3Di3.40.50.1580. 1 hit.
HAMAPiMF_01963. MTAP. 1 hit.
InterProiIPR010044. MTAP.
IPR000845. Nucleoside_phosphorylase_d.
IPR001369. PNP/MTAP.
[Graphical view]
PANTHERiPTHR11904. PTHR11904. 1 hit.
PfamiPF01048. PNP_UDP_1. 1 hit.
[Graphical view]
SUPFAMiSSF53167. SSF53167. 1 hit.
TIGRFAMsiTIGR01694. MTAP. 1 hit.

Sequencei

Sequence statusi: Complete.

B5YKP5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHNKQQVKIG IIGGSGLSES EAKKEIITIK TPYGEPSCPY EIEKIDDIEV
60 70 80 90 100
LFLRRHGQKH SIPPHKVNYR ANIYGFKNFG IERIFGVFAT GSLTENIPPG
110 120 130 140 150
SIVIPNQIID FTQGMRANTF YEEKKVVHID FTEPFCSEIR HYLLETARKI
160 170 180 190 200
GINVISHATY ICVNGPRLET AAEIKFFKNI GADIIGMTIM PEASLAREVE
210 220 230 240 250
ICYAAVAVVA NYAAGISKFP LTVKEVIETM EDSLDAVGFL IKETIKKLPE
260
ERKCLCKHAL KNASF
Length:265
Mass (Da):29,620
Last modified:November 25, 2008 - v1
Checksum:iB109DB0BD2497606
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001147 Genomic DNA. Translation: ACI21697.1.
RefSeqiWP_012546405.1. NC_011296.1.
YP_002248810.1. NC_011296.1.

Genome annotation databases

EnsemblBacteriaiACI21697; ACI21697; THEYE_A0977.
GeneIDi6941541.
KEGGitye:THEYE_A0977.
PATRICi23908977. VBITheYel104483_0962.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001147 Genomic DNA. Translation: ACI21697.1.
RefSeqiWP_012546405.1. NC_011296.1.
YP_002248810.1. NC_011296.1.

3D structure databases

ProteinModelPortaliB5YKP5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi289376.THEYE_A0977.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACI21697; ACI21697; THEYE_A0977.
GeneIDi6941541.
KEGGitye:THEYE_A0977.
PATRICi23908977. VBITheYel104483_0962.

Phylogenomic databases

eggNOGiENOG4107RHB. Bacteria.
COG0005. LUCA.
HOGENOMiHOG000228987.
InParanoidiB5YKP5.
KOiK00772.
OMAiGCDIVGM.
OrthoDBiPOG091H0228.

Enzyme and pathway databases

UniPathwayiUPA00606.
BioCyciTYEL289376:GH9L-973-MONOMER.

Family and domain databases

Gene3Di3.40.50.1580. 1 hit.
HAMAPiMF_01963. MTAP. 1 hit.
InterProiIPR010044. MTAP.
IPR000845. Nucleoside_phosphorylase_d.
IPR001369. PNP/MTAP.
[Graphical view]
PANTHERiPTHR11904. PTHR11904. 1 hit.
PfamiPF01048. PNP_UDP_1. 1 hit.
[Graphical view]
SUPFAMiSSF53167. SSF53167. 1 hit.
TIGRFAMsiTIGR01694. MTAP. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMTIP_THEYD
AccessioniPrimary (citable) accession number: B5YKP5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 25, 2012
Last sequence update: November 25, 2008
Last modified: September 7, 2016
This is version 43 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Although this enzyme belongs to the family of MTA phosphorylases based on sequence homology, it has been shown that conserved amino acid substitutions in the substrate binding pocket convert the substrate specificity of this enzyme from 6-aminopurines to 6-oxopurines.UniRule annotation

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.