B5YK85 (BIOB_THEYD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Biotin synthase
EC=2.8.1.6

Gene names

Name:	bioB
Ordered Locus Names:	THEYE_A0809

Organism

Thermodesulfovibrio yellowstonii (strain ATCC 51303 / DSM 11347 / YP87) [Reference proteome] [HAMAP]

Taxonomic identifier

289376 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Nitrospirae › Nitrospira › Nitrospirales › Nitrospiraceae › Thermodesulfovibrio › Thermodesulfovibrio yellowstonii

Protein attributes

Sequence length	316 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity. HAMAP-Rule MF_01694
Catalytic activity	Dethiobiotin + sulfur + 2 S-adenosyl-L-methionine = biotin + 2 L-methionine + 2 5'-deoxyadenosine. HAMAP-Rule MF_01694
Cofactor	Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity. Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.
Pathway	Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 2/2. HAMAP-Rule MF_01694
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the radical SAM superfamily. Biotin synthase family.

Ontologies

Keywords
Biological process	Biotin biosynthesis
Ligand	2Fe-2S 4Fe-4S Iron Iron-sulfur Metal-binding S-adenosyl-L-methionine
Molecular function	Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	biotin biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular_function	2 iron, 2 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW 4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW biotin synthase activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 316	316	Biotin synthase HAMAP-Rule MF_01694		PRO_0000381688
Sites
	Metal binding	56	1	Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
	Metal binding	60	1	Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
	Metal binding	63	1	Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
	Metal binding	100	1	Iron-sulfur 2 (2Fe-2S) By similarity
	Metal binding	131	1	Iron-sulfur 2 (2Fe-2S) By similarity
	Metal binding	191	1	Iron-sulfur 2 (2Fe-2S) By similarity
	Metal binding	261	1	Iron-sulfur 2 (2Fe-2S) By similarity

Sequences

	Sequence		Length	Mass (Da)
	B5YK85 [UniParc]. Last modified November 25, 2008. Version 1. Checksum: 98E6617E191A4D0B	FASTA	316	35,460
	10 20 30 40 50 60 MKPEIQSSLE YYSDLIEHMD DSHIYSLIFQ ANSIREKYKG KKIELCAIVN AKSGLCSEDC 70 80 90 100 110 120 AFCAQSSRYK TNSPVYSLLE KDEIVKKALE AKKYGVKRFS IVISGKKPSK KELEKIGNSI 130 140 150 160 170 180 EAIKKIGIYP CASLGLLEYD EICYLRDKGL ERLHCNIETS ERFFSNICTT HKFSDKVKTL 190 200 210 220 230 240 ENANKTGLSI CSGGVFGIGE SWDDRKEMAE FLKNLNVDSI PINFLTPIKG TPLESKKPLN 250 260 270 280 290 300 PMEALRIIIL FRLILPDKDI RVCGGRPLLG EFASWIFIAG ANALMTGNYL TTTGRHYSDD 310 IKFIEAHGLE VDSVVS

References

[1]

"The complete genome sequence of Thermodesulfovibrio yellowstonii strain ATCC 51303 / DSM 11347 / YP87." Dodson R.J., Durkin A.S., Wu M., Eisen J., Sutton G. Submitted (AUG-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 51303 / DSM 11347 / YP87.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001147 Genomic DNA. Translation: ACI22036.1.
RefSeq	YP_002248650.1. NC_011296.1.
3D structure databases
ProteinModelPortal	B5YK85.
ModBase	Search...
Protein-protein interaction databases
STRING	289376.THEYE_A0809.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACI22036; ACI22036; THEYE_A0809.
GeneID	6942028.
KEGG	tye:THEYE_A0809.
PATRIC	23908639. VBITheYel104483_0799.
Phylogenomic databases
eggNOG	COG0502.
HOGENOM	HOG000239958.
KO	K01012.
OMA	MKETKQD.
Enzyme and pathway databases
UniPathway	UPA00078; UER00162.
Family and domain databases
Gene3D	3.20.20.70. 1 hit.
HAMAP	MF_01694. BioB.
InterPro	IPR013785. Aldolase_TIM. IPR010722. Biotin/thiamin_synth-assoc. IPR002684. Biotin_synth/BioAB. IPR024177. Biotin_synthase. IPR006638. Elp3/MiaB/NifB. IPR007197. rSAM. [Graphical view]
Pfam	PF06968. BATS. 1 hit. PF04055. Radical_SAM. 1 hit. [Graphical view]
PIRSF	PIRSF001619. Biotin_synth. 1 hit.
SMART	SM00876. BATS. 1 hit. SM00729. Elp3. 1 hit. [Graphical view]
TIGRFAMs	TIGR00433. bioB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

BIOB_THEYD

Accession

Primary (citable) accession number: B5YK85

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
Last sequence update:	November 25, 2008
Last modified:	May 1, 2013
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program