Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

B5YJZ3 (HEM1_THEYD) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 45. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutamyl-tRNA reductase

Short name=GluTR
EC=1.2.1.70
Gene names
Name:hemA
Ordered Locus Names:THEYE_A0716
OrganismThermodesulfovibrio yellowstonii (strain ATCC 51303 / DSM 11347 / YP87) [Reference proteome] [HAMAP]
Taxonomic identifier289376 [NCBI]
Taxonomic lineageBacteriaNitrospiraeNitrospiralesNitrospiraceaeThermodesulfovibrio

Protein attributes

Sequence length429 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the NADPH-dependent reduction of glutamyl-tRNA(Glu) to glutamate 1-semialdehyde (GSA) By similarity. HAMAP-Rule MF_00087

Catalytic activity

L-glutamate 1-semialdehyde + NADP+ + tRNA(Glu) = L-glutamyl-tRNA(Glu) + NADPH. HAMAP-Rule MF_00087

Pathway

Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; 5-aminolevulinate from L-glutamyl-tRNA(Glu): step 1/2. HAMAP-Rule MF_00087

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_00087

Domain

Possesses an unusual extended V-shaped dimeric structure with each monomer consisting of three distinct domains arranged along a curved 'spinal' alpha-helix. The N-terminal catalytic domain specifically recognizes the glutamate moiety of the substrate. The second domain is the NADPH-binding domain, and the third C-terminal domain is responsible for dimerization By similarity. HAMAP-Rule MF_00087

Miscellaneous

During catalysis, the active site Cys acts as a nucleophile attacking the alpha-carbonyl group of tRNA-bound glutamate with the formation of a thioester intermediate between enzyme and glutamate, and the concomitant release of tRNA(Glu). The thioester intermediate is finally reduced by direct hydride transfer from NADPH, to form the product GSA By similarity.

Sequence similarities

Belongs to the glutamyl-tRNA reductase family.

Ontologies

Keywords
   Biological processPorphyrin biosynthesis
   LigandNADP
   Molecular functionOxidoreductase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processprotoporphyrinogen IX biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Molecular_functionNADP binding

Inferred from electronic annotation. Source: InterPro

glutamyl-tRNA reductase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 429429Glutamyl-tRNA reductase HAMAP-Rule MF_00087
PRO_1000093176

Regions

Nucleotide binding196 – 2016NADP By similarity
Region50 – 534Substrate binding By similarity
Region121 – 1233Substrate binding By similarity

Sites

Active site511Nucleophile By similarity
Binding site1161Substrate By similarity
Binding site1271Substrate By similarity
Site1061Important for activity By similarity

Sequences

Sequence LengthMass (Da)Tools
B5YJZ3 [UniParc].

Last modified November 25, 2008. Version 1.
Checksum: BEF10E3F580CE867

FASTA42948,820
        10         20         30         40         50         60 
MSLIVIGLNH KTAPVEIREK IAFNSKEAIK EALKELIQRE GIGEVVIIST CNRVEIYVYT 

        70         80         90        100        110        120 
ANVSDLKREN QVEETIKAFL SNFHNIEIGE FENYLYVYKD TEAVEHLFKV ASSLDSMIVG 

       130        140        150        160        170        180 
EPQITGQVKE SYEIALSERT TSLILNYLMN RALFTAKRVR NETRIGENPV SVSYAAVGLI 

       190        200        210        220        230        240 
KKVFDELSKK SILLVGAGEM AELALRHLIG SGIKNVYLTN RTFQRAEEIA KEFNGVAVPF 

       250        260        270        280        290        300 
GNLKEQLVKT DIVICSTGAP HYVITEQMLK EVMPLRKHKP IFFIDISVPR NVDPACNELD 

       310        320        330        340        350        360 
NVYLYNIDDL QDVVDSNILE RKKEAEKALS IVQEETEKFF QWLNSLESVP VIVSIRNKAE 

       370        380        390        400        410        420 
QVRQEEIEKF KAKYKDLPPE LINSIDYLTQ SIINKIMHSP TVALKNNCEN KEILIFSARR 


LFGLDSEEE 

« Hide

References

[1]"The complete genome sequence of Thermodesulfovibrio yellowstonii strain ATCC 51303 / DSM 11347 / YP87."
Dodson R.J., Durkin A.S., Wu M., Eisen J., Sutton G.
Submitted (AUG-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 51303 / DSM 11347 / YP87.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001147 Genomic DNA. Translation: ACI21817.1.
RefSeqYP_002248558.1. NC_011296.1.

3D structure databases

ProteinModelPortalB5YJZ3.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING289376.THEYE_A0716.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACI21817; ACI21817; THEYE_A0716.
GeneID6941976.
KEGGtye:THEYE_A0716.
PATRIC23908453. VBITheYel104483_0708.

Phylogenomic databases

eggNOGCOG0373.
HOGENOMHOG000109651.
KOK02492.
OMARTEIYCA.
OrthoDBEOG6MWNBM.

Enzyme and pathway databases

BioCycTYEL289376:GH9L-714-MONOMER.
UniPathwayUPA00251; UER00316.

Family and domain databases

Gene3D3.40.50.720. 1 hit.
HAMAPMF_00087. Glu_tRNA_reductase.
InterProIPR000343. 4pyrrol_synth_GluRdtase.
IPR015896. 4pyrrol_synth_GluRdtase_dimer.
IPR015895. 4pyrrol_synth_GluRdtase_N.
IPR016040. NAD(P)-bd_dom.
IPR018214. Pyrrol_synth_GluRdtase_CS.
IPR006151. Shikm_DH/Glu-tRNA_Rdtase.
[Graphical view]
PfamPF00745. GlutR_dimer. 1 hit.
PF05201. GlutR_N. 1 hit.
PF01488. Shikimate_DH. 1 hit.
[Graphical view]
PIRSFPIRSF000445. 4pyrrol_synth_GluRdtase. 1 hit.
SUPFAMSSF69075. SSF69075. 1 hit.
SSF69742. SSF69742. 1 hit.
TIGRFAMsTIGR01035. hemA. 1 hit.
PROSITEPS00747. GLUTR. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameHEM1_THEYD
AccessionPrimary (citable) accession number: B5YJZ3
Entry history
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: November 25, 2008
Last modified: February 19, 2014
This is version 45 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways