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B5YJD3 (METE_THEYD) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 42. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase

EC=2.1.1.14
Alternative name(s):
Cobalamin-independent methionine synthase
Methionine synthase, vitamin-B12 independent isozyme
Gene names
Name:metE
Ordered Locus Names:THEYE_A0504
OrganismThermodesulfovibrio yellowstonii (strain ATCC 51303 / DSM 11347 / YP87) [Reference proteome] [HAMAP]
Taxonomic identifier289376 [NCBI]
Taxonomic lineageBacteriaNitrospiraeNitrospiralesNitrospiraceaeThermodesulfovibrio

Protein attributes

Sequence length745 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the transfer of a methyl group from 5-methyltetrahydrofolate to homocysteine resulting in methionine formation By similarity. HAMAP-Rule MF_00172

Catalytic activity

5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine = tetrahydropteroyltri-L-glutamate + L-methionine. HAMAP-Rule MF_00172

Cofactor

Binds 1 zinc ion per subunit By similarity. HAMAP-Rule MF_00172

Pathway

Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-methionine from L-homocysteine (MetE route): step 1/1. HAMAP-Rule MF_00172

Sequence similarities

Belongs to the vitamin-B12 independent methionine synthase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 7457455-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase HAMAP-Rule MF_00172
PRO_1000097850

Sites

Metal binding6311Zinc By similarity
Metal binding6331Zinc By similarity
Metal binding7161Zinc By similarity

Sequences

Sequence LengthMass (Da)Tools
B5YJD3 [UniParc].

Last modified November 25, 2008. Version 1.
Checksum: C4C8F5367B4EF5BD

FASTA74585,984
        10         20         30         40         50         60 
MKIKTTVFGY PRIGPKRELK KALEDYWNWK ISKVELLETA NSLIIQNAKV IQSSGVDLIP 

        70         80         90        100        110        120 
SNEFSLYDFI LDHSVMFNAV PKRFNRISDP LDRYFAMARG TQELPALEMT KWFNTNYHYI 

       130        140        150        160        170        180 
VPEIEDEEFE LMENKPLREF ALLRDSLSVK TKPVIVGPFT YLKSAKLNMD RVERLSEKIL 

       190        200        210        220        230        240 
PAYKKLLIKL DAAGVEEIQI DEPAMVMDME EREIELLTGL YRELTKGLTL KVYIQTYYEA 

       250        260        270        280        290        300 
VSAYEKLVFS LSVHGFGFDL VDGKENLENI LTLGFPSDKV LIAGVVSGRD PWRTDFTVVM 

       310        320        330        340        350        360 
SMLERLFRVT DKIMLSNSSP LIHLPITVEA ERGHIQEDIL NMLSFANERL EELTILKRAI 

       370        380        390        400        410        420 
NDGAALPARK SIVHEIFSKA EVRSKISSID EKAILRKPEF QERYRMQMDS LKLPLFPTTT 

       430        440        450        460        470        480 
IGSFPQTKEV RKFRADYKNG KITEEEYKKF IFQEIEKAVK IQEELDIDVL VHGEFERTDM 

       490        500        510        520        530        540 
VEFFAEKLKG FAITKNGWVQ SYGSRCVRPP VIYGDVWRDK ALTVEETLYA QSLTPRPVKG 

       550        560        570        580        590        600 
IMTGAVTILQ WSYPRKDISR REIAYQIALA LKEEVLELEK RGIKIIQIDE PAFREGLPLK 

       610        620        630        640        650        660 
RNKQDEYFDW TINSFRLTTA DVSPFTQIHT HMCYSDFNEI IDRIYALDAD VISIEASRSK 

       670        680        690        700        710        720 
GEILSAFENF RYDHGIGLGV YDIHSPRVPS VEEMIEIIER SVSLIDKSLF WINPDCGLKT 

       730        740 
RGWQETVASL KNMVLAAKAM RKREV 

« Hide

References

[1]"The complete genome sequence of Thermodesulfovibrio yellowstonii strain ATCC 51303 / DSM 11347 / YP87."
Dodson R.J., Durkin A.S., Wu M., Eisen J., Sutton G.
Submitted (AUG-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 51303 / DSM 11347 / YP87.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001147 Genomic DNA. Translation: ACI21086.1.
RefSeqYP_002248348.1. NC_011296.1.

3D structure databases

ProteinModelPortalB5YJD3.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING289376.THEYE_A0504.

Proteomic databases

PRIDEB5YJD3.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACI21086; ACI21086; THEYE_A0504.
GeneID6943070.
KEGGtye:THEYE_A0504.
PATRIC23908031. VBITheYel104483_0499.

Phylogenomic databases

eggNOGCOG0620.
HOGENOMHOG000246221.
KOK00549.
OMASFPQTSE.
OrthoDBEOG6FFS3G.
ProtClustDBPRK05222.

Enzyme and pathway databases

BioCycTYEL289376:GH9L-502-MONOMER.
UniPathwayUPA00051; UER00082.

Family and domain databases

HAMAPMF_00172. Meth_synth.
InterProIPR013215. Cbl-indep_Met_Synth_N.
IPR006276. Cobalamin-indep_Met_synthase.
IPR002629. Methionine_synth.
[Graphical view]
PfamPF08267. Meth_synt_1. 1 hit.
PF01717. Meth_synt_2. 1 hit.
[Graphical view]
PIRSFPIRSF000382. MeTrfase_B12_ind. 1 hit.
TIGRFAMsTIGR01371. met_syn_B12ind. 1 hit.
ProtoNetSearch...

Entry information

Entry nameMETE_THEYD
AccessionPrimary (citable) accession number: B5YJD3
Entry history
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: November 25, 2008
Last modified: February 19, 2014
This is version 42 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways