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Protein

Pyrophosphate--fructose 6-phosphate 1-phosphotransferase

Gene

pfp

Organism
Thermodesulfovibrio yellowstonii (strain ATCC 51303 / DSM 11347 / YP87)
Status
Unreviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate, the first committing step of glycolysis. Uses inorganic phosphate (PPi) as phosphoryl donor instead of ATP like common ATP-dependent phosphofructokinases (ATP-PFKs), which renders the reaction reversible, and can thus function both in glycolysis and gluconeogenesis. Consistently, PPi-PFK can replace the enzymes of both the forward (ATP-PFK) and reverse (fructose-bisphosphatase (FBPase)) reactions.UniRule annotation

Catalytic activityi

Diphosphate + D-fructose 6-phosphate = phosphate + D-fructose 1,6-bisphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Enzyme regulationi

Non-allosteric.UniRule annotation

Pathway: glycolysis

This protein is involved in step 3 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose.UniRule annotation
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Glucose-6-phosphate isomerase (pgi), Glucose-6-phosphate isomerase (pgi)
  3. Pyrophosphate--fructose 6-phosphate 1-phosphotransferase (pfp)
  4. no protein annotated in this organism
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei11 – 111Diphosphate; via amide nitrogenUniRule annotation
Metal bindingi106 – 1061Magnesium; catalyticUniRule annotation
Sitei130 – 1301Important for catalytic activity; stabilizes the transition state when the phosphoryl donor is PPiUniRule annotation
Active sitei133 – 1331Proton acceptorUniRule annotation
Binding sitei238 – 2381SubstrateUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

KinaseUniRule annotation, Transferase

Keywords - Biological processi

GlycolysisUniRule annotation

Keywords - Ligandi

MagnesiumUniRule annotation, Metal-bindingUniRule annotation

Enzyme and pathway databases

BioCyciTYEL289376:GH9L-1937-MONOMER.
UniPathwayiUPA00109; UER00182.

Names & Taxonomyi

Protein namesi
Recommended name:
Pyrophosphate--fructose 6-phosphate 1-phosphotransferaseUniRule annotation (EC:2.7.1.90UniRule annotation)
Alternative name(s):
6-phosphofructokinase, pyrophosphate dependentUniRule annotation
PPi-dependent phosphofructokinaseUniRule annotation
Pyrophosphate-dependent 6-phosphofructose-1-kinaseUniRule annotation
Gene namesi
Name:pfpUniRule annotation
Ordered Locus Names:THEYE_A1948Imported
OrganismiThermodesulfovibrio yellowstonii (strain ATCC 51303 / DSM 11347 / YP87)Imported
Taxonomic identifieri289376 [NCBI]
Taxonomic lineageiBacteriaNitrospiraeNitrospiralesNitrospiraceaeThermodesulfovibrio
ProteomesiUP000000718 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotation

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi289376.THEYE_A1948.

Structurei

3D structure databases

ProteinModelPortaliB5YIA7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni131 – 1333Substrate bindingUniRule annotation
Regioni177 – 1793Substrate bindingUniRule annotation
Regioni298 – 3014Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the phosphofructokinase type A (PFKA) family. PPi-dependent PFK group II subfamily. Clade "Short" sub-subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0205.
HOGENOMiHOG000279330.
InParanoidiB5YIA7.
KOiK00850.
OMAiEYTRNLG.
OrthoDBiEOG6WDSC8.

Family and domain databases

HAMAPiMF_01979. Phosphofructokinase_II_Short.
InterProiIPR022953. ATP_PFK.
IPR000023. Phosphofructokinase_dom.
IPR011403. PPi-PFK_TM0289.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PIRSFiPIRSF036482. PPi_PFK_TM0289. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.

Sequencei

Sequence statusi: Complete.

B5YIA7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
METVGIIVGG GPAPGINGTI SAATIEAINQ GKKVIGIKGG FKPLFDGNLS
60 70 80 90 100
CAMPLSVDDV SRIHTKGGSI LRTSREHAER VKEKFPVLME TLKKLGIKYL
110 120 130 140 150
ITIGGDGTLF MANWIEREAR GEINVVHVPK TIDNDLPLPG GASTFGYETA
160 170 180 190 200
RHWGVEIVKN IMEDARVSAR WYFLTIMGRY TGHLALGVGK AAGATLTIIP
210 220 230 240 250
EEFPEEKISF KKVADILTGA IIKRLSMGRD HGVVIMAEGL SEKFDPEELS
260 270 280 290 300
HYEQLEKDET GRVRLSEIQL GRIMKNFVKK SLEDMGIKLT IVDKNIGYEL
310 320 330 340 350
RSADPIPYDI EYTRNLGYGA VRYLLRGGTG AMITFEEGHL RPIPFVELID
360 370 380 390 400
YRTGKVKIRK VDITSENYEV GRKYMIRLEA EDFEGERLKG LASVVKMSEK
410
EFREKFFYVT T
Length:411
Mass (Da):45,779
Last modified:November 25, 2008 - v1
Checksum:i163B1B13E752A258
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001147 Genomic DNA. Translation: ACI21803.1.
RefSeqiWP_012546508.1. NC_011296.1.
YP_002249738.1. NC_011296.1.

Genome annotation databases

EnsemblBacteriaiACI21803; ACI21803; THEYE_A1948.
GeneIDi6942646.
KEGGitye:THEYE_A1948.
PATRICi23910923. VBITheYel104483_1903.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001147 Genomic DNA. Translation: ACI21803.1.
RefSeqiWP_012546508.1. NC_011296.1.
YP_002249738.1. NC_011296.1.

3D structure databases

ProteinModelPortaliB5YIA7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi289376.THEYE_A1948.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACI21803; ACI21803; THEYE_A1948.
GeneIDi6942646.
KEGGitye:THEYE_A1948.
PATRICi23910923. VBITheYel104483_1903.

Phylogenomic databases

eggNOGiCOG0205.
HOGENOMiHOG000279330.
InParanoidiB5YIA7.
KOiK00850.
OMAiEYTRNLG.
OrthoDBiEOG6WDSC8.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00182.
BioCyciTYEL289376:GH9L-1937-MONOMER.

Family and domain databases

HAMAPiMF_01979. Phosphofructokinase_II_Short.
InterProiIPR022953. ATP_PFK.
IPR000023. Phosphofructokinase_dom.
IPR011403. PPi-PFK_TM0289.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PIRSFiPIRSF036482. PPi_PFK_TM0289. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "The complete genome sequence of Thermodesulfovibrio yellowstonii strain ATCC 51303 / DSM 11347 / YP87."
    Dodson R.J., Durkin A.S., Wu M., Eisen J., Sutton G.
    Submitted (AUG-2008) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 51303 / DSM 11347 / YP87Imported.

Entry informationi

Entry nameiB5YIA7_THEYD
AccessioniPrimary (citable) accession number: B5YIA7
Entry historyi
Integrated into UniProtKB/TrEMBL: November 25, 2008
Last sequence update: November 25, 2008
Last modified: May 27, 2015
This is version 41 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.