B5YFC7 (B5YFC7_DICT6) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
DNA topoisomerase RuleBase RU000518
EC=5.99.1.2 RuleBase RU000518

Gene names

Name:	topA EMBL ACI19958.1
Ordered Locus Names:	DICTH_1416

Organism

Dictyoglomus thermophilum (strain ATCC 35947 / DSM 3960 / H-6-12) [Complete proteome] [HAMAP]

Taxonomic identifier

309799 [NCBI]

Taxonomic lineage

Bacteria › Dictyoglomi › Dictyoglomales › Dictyoglomaceae › Dictyoglomus

Protein attributes

Sequence length	694 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity. SAAS SAAS000380
Catalytic activity	ATP-independent breakage of single-stranded DNA, followed by passage and rejoining. RuleBase RU000518 SAAS SAAS005733
Sequence similarities	Belongs to the prokaryotic type I/III topoisomerase family. RuleBase RU004206 Contains 1 Toprim domain. SAAS SAAS023406

Ontologies

Keywords
Ligand	ATP-binding SAAS SAAS005733 DNA-binding RuleBase RU000518 SAAS SAAS005733 Nucleotide-binding
Molecular function	Isomerase Topoisomerase RuleBase RU000518 SAAS SAAS005733
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase type I activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	B5YFC7 [UniParc]. Last modified November 25, 2008. Version 1. Checksum: E703DF2D62A14B7B	FASTA	694	80,534
	10 20 30 40 50 60 MSKEKLIIVE SPAKAKTIEK ILGKDFKVLA SYGHIRDLPE KELGVDIENG FKPKYVLLPS 70 80 90 100 110 120 RKKIIDTIKD LSKKVEKLYL ATDPDREGEA IAWHLVETLK LPKDKFTRIE FHEITPRAIQ 130 140 150 160 170 180 EAIRNPRDID LDKVEAQQAR RILDRLVGYE LSPLLWEKVR RGLSAGRVQS VALKLICERE 190 200 210 220 230 240 EEIEKFVPEE YWYIYGEFED KDKIIYGKLV FYKEEEITIR NEKEAKRLIE VLNNLEYKID 250 260 270 280 290 300 RVVIKEEKKT PPLPFITSTL QQEASRRLGF PVSKTMKIAQ SLYEGVDIGD ERVGLITYMR 310 320 330 340 350 360 TDSTRIAPEA REEAVTYIES VFGKEYVGKE RKERKKLGMQ DAHEAIRPTS VLRTPEKVKK 370 380 390 400 410 420 YLTEDQYKLY KLIWERFLAS QMANALYDVI EIDIKGGDYT FVSKGRKLKF AGFMSLYTEK 430 440 450 460 470 480 EEEEEEEVNV DYIPQEGSDI DLINLTPKQF FTQPPPRYTE ATLVRTLEKY GIGRPSTYAT 490 500 510 520 530 540 IIETLKERKY VEVSKKELVP TTLGKVVNEF LTKQFPHLID VKFTAKMEED LDKIESGEVK 550 560 570 580 590 600 AIEVLEKFYP SFKEELERVS KNFGKIQVVE KTDEFCPICG RELVIRENKH GKYYACSAYP 610 620 630 640 650 660 ECKYTKPIYE EAHGKCPLCG GIVVKKKARK TGKVFWGCIN YPNCSFASPY EPLDEKCPQC 670 680 690 GSVLLKGNGF KKCSNENCNY IVWVGRRKRW KKSK

References

[1]	"The complete genome sequence of Dictyoglomus thermophilum strain ATCC 35947 / DSM 3960 / H-6-12." Dodson R.J., Durkin A.S., Wu M., Eisen J., Sutton G. Submitted (AUG-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001146 Genomic DNA. Translation: ACI19958.1.
RefSeq	YP_002251235.1. NC_011297.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	B5YFC7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6945452.
GenomeReviews	Gene locus DICTH_1416 in contig CP001146_GR.
KEGG	dth:DICTH_1416.
PATRIC	21807456. VBIDicThe33784_1359.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG512935.
OMA	EEYWSID.
ProtClustDB	CLSK2410035.
Family and domain databases
InterPro	IPR000380. Topo_IA. IPR003601. Topo_IA_2. IPR023406. Topo_IA_AS. IPR013497. Topo_IA_cen. IPR013824. Topo_IA_cen_sub1. IPR013825. Topo_IA_cen_sub2. IPR023405. Topo_IA_core_domain. IPR003602. Topo_IA_DNA-bd. IPR013498. Topo_IA_Znf. IPR005733. TopoI_bac-type. IPR006171. Toprim_domain. [Graphical view]
Gene3D	G3DSA:1.10.460.10. Topo_IA_cen_sub1. 3 hits. G3DSA:2.70.20.10. Topo_IA_cen_sub2. 2 hits.
KO	K03168.
PANTHER	PTHR11390. Topo_IA. 1 hit.
Pfam	PF01131. Topoisom_bac. 1 hit. PF01751. Toprim. 1 hit. PF01396. zf-C4_Topoisom. 2 hits. [Graphical view]
PRINTS	PR00417. PRTPISMRASEI.
SMART	SM00437. TOP1Ac. 1 hit. SM00436. TOP1Bc. 1 hit. SM00493. TOPRIM. 1 hit. [Graphical view]
SUPFAM	SSF56712. Topo_IA_core. 1 hit.
TIGRFAMs	TIGR01051. TopA_bact. 1 hit.
PROSITE	PS00396. TOPOISOMERASE_I_PROK. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

B5YFC7_DICT6

Accession

Primary (citable) accession number: B5YFC7

Entry history

Integrated into UniProtKB/TrEMBL:	November 25, 2008
Last sequence update:	November 25, 2008
Last modified:	December 14, 2011
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)