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Protein

Pyrophosphate--fructose 6-phosphate 1-phosphotransferase

Gene

pfp

Organism
Dictyoglomus thermophilum (strain ATCC 35947 / DSM 3960 / H-6-12)
Status
Unreviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate, the first committing step of glycolysis. Uses inorganic phosphate (PPi) as phosphoryl donor instead of ATP like common ATP-dependent phosphofructokinases (ATP-PFKs), which renders the reaction reversible, and can thus function both in glycolysis and gluconeogenesis. Consistently, PPi-PFK can replace the enzymes of both the forward (ATP-PFK) and reverse (fructose-bisphosphatase (FBPase)) reactions.UniRule annotation

Catalytic activityi

Diphosphate + D-fructose 6-phosphate = phosphate + D-fructose 1,6-bisphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Enzyme regulationi

Non-allosteric.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei13 – 131Diphosphate; via amide nitrogenUniRule annotation
Metal bindingi105 – 1051Magnesium; catalyticUniRule annotation
Sitei106 – 1061Important for catalytic activity and substrate specificity; stabilizes the transition state when the phosphoryl donor is PPi; prevents ATP from binding by mimicking the alpha-phosphate group of ATPUniRule annotation
Sitei126 – 1261Important for catalytic activity; stabilizes the transition state when the phosphoryl donor is PPiUniRule annotation
Active sitei129 – 1291Proton acceptorUniRule annotation
Binding sitei164 – 1641Substrate; shared with dimeric partnerUniRule annotation
Binding sitei224 – 2241SubstrateUniRule annotation
Binding sitei269 – 2691Substrate; shared with dimeric partnerUniRule annotation

GO - Molecular functioni

  1. 6-phosphofructokinase activity Source: InterPro
  2. ATP binding Source: UniProtKB-KW
  3. diphosphate-fructose-6-phosphate 1-phosphotransferase activity Source: UniProtKB-EC
  4. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. fructose 6-phosphate metabolic process Source: InterPro
  2. glycolytic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

KinaseUniRule annotation, Transferase

Keywords - Biological processi

GlycolysisUniRule annotationSAAS annotation

Keywords - Ligandi

ATP-bindingUniRule annotation, MagnesiumUniRule annotationSAAS annotation, Metal-bindingUniRule annotationSAAS annotation, Nucleotide-binding

Enzyme and pathway databases

BioCyciDTHE309799:GHF9-1264-MONOMER.
UniPathwayiUPA00109; UER00182.

Names & Taxonomyi

Protein namesi
Recommended name:
Pyrophosphate--fructose 6-phosphate 1-phosphotransferaseUniRule annotation (EC:2.7.1.90UniRule annotation)
Alternative name(s):
6-phosphofructokinase, pyrophosphate dependentUniRule annotation
PPi-dependent phosphofructokinaseUniRule annotation
Pyrophosphate-dependent 6-phosphofructose-1-kinaseUniRule annotation
Gene namesi
Name:pfpUniRule annotation
Ordered Locus Names:DICTH_1276Imported
OrganismiDictyoglomus thermophilum (strain ATCC 35947 / DSM 3960 / H-6-12)Imported
Taxonomic identifieri309799 [NCBI]
Taxonomic lineageiBacteriaDictyoglomiDictyoglomalesDictyoglomaceaeDictyoglomus
ProteomesiUP000001733 Componenti: Chromosome

Subcellular locationi

  1. Cytoplasm UniRule annotationSAAS annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotation

Interactioni

Subunit structurei

Homodimer or homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi309799.DICTH_1276.

Structurei

3D structure databases

ProteinModelPortaliB5YEZ1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni127 – 1293Substrate bindingUniRule annotation
Regioni171 – 1733Substrate bindingUniRule annotation
Regioni275 – 2784Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the phosphofructokinase type A (PFKA) family. Mixed-substrate PFK group III subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0205.
HOGENOMiHOG000248869.
KOiK00850.
OMAiKPAEGSM.
OrthoDBiEOG644ZRM.

Family and domain databases

HAMAPiMF_01976. Phosphofructokinase_III.
InterProiIPR022953. ATP_PFK.
IPR012003. ATP_PFK_prok-type.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
IPR012829. Phosphofructokinase_III.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PIRSFiPIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.
TIGRFAMsiTIGR02483. PFK_mixed. 1 hit.
PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B5YEZ1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKMRIGVLT GGGDCPGLNP AIRGIVMRAL DYGDEVIGLK YGWAGLLKAD
60 70 80 90 100
TMPLTLEMVE DILEIGGTIL GSSRTNPFKK EEDVQKCVEN FKKLNLDALI
110 120 130 140 150
AIGGEDTLGV ASKFSKLGLP MIGVPKTIDK DLEETDYTLG FDTAVEVVVD
160 170 180 190 200
AIKRLRDTAR SHARVIVVEI MGRHAGWLAL YGGLAGGADY ILIPEVEPNL
210 220 230 240 250
EDLYNHIRKL YARGRNHAVV AIAEGVQLPG FTYQKGQEGM VDAFGHIRLG
260 270 280 290 300
GVGNVLAEEI QKNLGIETRA VILSHLQRGG SPSIRDRIMG LLLGKKAVDL
310 320 330 340
VHEGKSGLFV AVKGNELVPV DITLIEGKTK NVDPAFYESV KTFFNK
Length:346
Mass (Da):37,462
Last modified:November 25, 2008 - v1
Checksum:iD61B5535BB3C32A3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001146 Genomic DNA. Translation: ACI18474.1.
RefSeqiYP_002251099.1. NC_011297.1.

Genome annotation databases

EnsemblBacteriaiACI18474; ACI18474; DICTH_1276.
KEGGidth:DICTH_1276.
PATRICi21807178. VBIDicThe33784_1223.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001146 Genomic DNA. Translation: ACI18474.1.
RefSeqiYP_002251099.1. NC_011297.1.

3D structure databases

ProteinModelPortaliB5YEZ1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi309799.DICTH_1276.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACI18474; ACI18474; DICTH_1276.
KEGGidth:DICTH_1276.
PATRICi21807178. VBIDicThe33784_1223.

Phylogenomic databases

eggNOGiCOG0205.
HOGENOMiHOG000248869.
KOiK00850.
OMAiKPAEGSM.
OrthoDBiEOG644ZRM.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00182.
BioCyciDTHE309799:GHF9-1264-MONOMER.

Family and domain databases

HAMAPiMF_01976. Phosphofructokinase_III.
InterProiIPR022953. ATP_PFK.
IPR012003. ATP_PFK_prok-type.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
IPR012829. Phosphofructokinase_III.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PIRSFiPIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.
TIGRFAMsiTIGR02483. PFK_mixed. 1 hit.
PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The complete genome sequence of Dictyoglomus thermophilum strain ATCC 35947 / DSM 3960 / H-6-12."
    Dodson R.J., Durkin A.S., Wu M., Eisen J., Sutton G.
    Submitted (AUG-2008) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 35947 / DSM 3960 / H-6-12Imported.

Entry informationi

Entry nameiB5YEZ1_DICT6
AccessioniPrimary (citable) accession number: B5YEZ1
Entry historyi
Integrated into UniProtKB/TrEMBL: November 25, 2008
Last sequence update: November 25, 2008
Last modified: April 29, 2015
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.