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Protein

Glutamate--tRNA ligase

Gene

gltX

Organism
Dictyoglomus thermophilum (strain ATCC 35947 / DSM 3960 / H-6-12)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).UniRule annotation

Catalytic activityi

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei245ATPUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciDTHE309799:G1GCP-1129-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate--tRNA ligaseUniRule annotation (EC:6.1.1.17UniRule annotation)
Alternative name(s):
Glutamyl-tRNA synthetaseUniRule annotation
Short name:
GluRSUniRule annotation
Gene namesi
Name:gltXUniRule annotation
Ordered Locus Names:DICTH_1169
OrganismiDictyoglomus thermophilum (strain ATCC 35947 / DSM 3960 / H-6-12)
Taxonomic identifieri309799 [NCBI]
Taxonomic lineageiBacteriaDictyoglomiDictyoglomalesDictyoglomaceaeDictyoglomus
Proteomesi
  • UP000001733 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000900711 – 477Glutamate--tRNA ligaseAdd BLAST477

Proteomic databases

PRIDEiB5YEP5

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi309799.DICTH_1169

Structurei

3D structure databases

ProteinModelPortaliB5YEP5
SMRiB5YEP5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi10 – 20"HIGH" regionUniRule annotationAdd BLAST11
Motifi242 – 246"KMSKS" regionUniRule annotation5

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. Glutamate--tRNA ligase type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C20 Bacteria
COG0008 LUCA
HOGENOMiHOG000252720
KOiK01885
OMAiAYYAFDT
OrthoDBiPOG091H021W

Family and domain databases

CDDicd00808 GluRS_core, 1 hit
Gene3Di1.10.10.350, 1 hit
3.40.50.620, 1 hit
HAMAPiMF_00022 Glu_tRNA_synth_type1, 1 hit
InterProiView protein in InterPro
IPR008925 aa-tRNA-synth_I_codon-bd
IPR020751 aa-tRNA-synth_I_codon-bd_sub2
IPR001412 aa-tRNA-synth_I_CS
IPR004527 Glu-tRNA-ligase_bac/mito
IPR000924 Glu/Gln-tRNA-synth
IPR020058 Glu/Gln-tRNA-synth_Ib_cat-dom
IPR033910 GluRS_core
IPR014729 Rossmann-like_a/b/a_fold
PfamiView protein in Pfam
PF00749 tRNA-synt_1c, 1 hit
PRINTSiPR00987 TRNASYNTHGLU
SUPFAMiSSF48163 SSF48163, 1 hit
TIGRFAMsiTIGR00464 gltX_bact, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

B5YEP5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDNVRVRIAP SPTGYLHVGT AHTALFNWLF ARHNKGTFIL RIEDTDLARS
60 70 80 90 100
TKEYEENIIE GLKWLGLYWD EGPYYQTQRL DLYRKYADEL LKKGLAYYCY
110 120 130 140 150
CTPEELEERR KEALAQKKPP RYDRKCLYLS DEERARYERE GRKPAIRLLV
160 170 180 190 200
PEGKTVFNDI IRGEIEFNNE DIGDFVIMKS DGIPTYNFAV VIDDYTMGIT
210 220 230 240 250
HVIRGEDHIS NTPKQLFIYN AFGWTPPEFA HLPLLLGPDR SKLSKRHGVT
260 270 280 290 300
SVTEYKKMGY LPQALVNYLA LLGWTPEENR EIYTVEELIE LFDLRRVTKN
310 320 330 340 350
PGIFDVTKLE WINTQHIRRL SLEELADLTY PFLKEAEWFK EENFNKEYYL
360 370 380 390 400
KVLSLLQERL KTLAQIKEYA DYFFVDDLDY DVETVKKVCK HPDTPLYLEK
410 420 430 440 450
IKDVWSQLSE FTAENLEASL KKLAEENQIP TKNLVHPIRV ALTNKTVGPG
460 470
LYEIAEVLGK EKTLKRLEKF IQFLKTL
Length:477
Mass (Da):55,994
Last modified:November 25, 2008 - v1
Checksum:i290EF4E4EC661D2A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001146 Genomic DNA Translation: ACI20166.1
RefSeqiWP_012548798.1, NC_011297.1

Genome annotation databases

EnsemblBacteriaiACI20166; ACI20166; DICTH_1169
KEGGidth:DICTH_1169

Similar proteinsi

Entry informationi

Entry nameiSYE_DICT6
AccessioniPrimary (citable) accession number: B5YEP5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: November 25, 2008
Last modified: May 23, 2018
This is version 60 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families
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