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B5YEP5 (SYE_DICT6) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 39. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutamate--tRNA ligase

EC=6.1.1.17
Alternative name(s):
Glutamyl-tRNA synthetase
Short name=GluRS
Gene names
Name:gltX
Ordered Locus Names:DICTH_1169
OrganismDictyoglomus thermophilum (strain ATCC 35947 / DSM 3960 / H-6-12) [Complete proteome] [HAMAP]
Taxonomic identifier309799 [NCBI]
Taxonomic lineageBacteriaDictyoglomiDictyoglomalesDictyoglomaceaeDictyoglomus

Protein attributes

Sequence length477 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity. HAMAP-Rule MF_00022

Catalytic activity

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP-Rule MF_00022

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00022

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00022.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processglutamyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

glutamate-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

tRNA binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 477477Glutamate--tRNA ligase HAMAP-Rule MF_00022
PRO_1000090071

Regions

Motif10 – 2011"HIGH" region HAMAP-Rule MF_00022
Motif242 – 2465"KMSKS" region HAMAP-Rule MF_00022

Sites

Binding site2451ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
B5YEP5 [UniParc].

Last modified November 25, 2008. Version 1.
Checksum: 290EF4E4EC661D2A

FASTA47755,994
        10         20         30         40         50         60 
MDNVRVRIAP SPTGYLHVGT AHTALFNWLF ARHNKGTFIL RIEDTDLARS TKEYEENIIE 

        70         80         90        100        110        120 
GLKWLGLYWD EGPYYQTQRL DLYRKYADEL LKKGLAYYCY CTPEELEERR KEALAQKKPP 

       130        140        150        160        170        180 
RYDRKCLYLS DEERARYERE GRKPAIRLLV PEGKTVFNDI IRGEIEFNNE DIGDFVIMKS 

       190        200        210        220        230        240 
DGIPTYNFAV VIDDYTMGIT HVIRGEDHIS NTPKQLFIYN AFGWTPPEFA HLPLLLGPDR 

       250        260        270        280        290        300 
SKLSKRHGVT SVTEYKKMGY LPQALVNYLA LLGWTPEENR EIYTVEELIE LFDLRRVTKN 

       310        320        330        340        350        360 
PGIFDVTKLE WINTQHIRRL SLEELADLTY PFLKEAEWFK EENFNKEYYL KVLSLLQERL 

       370        380        390        400        410        420 
KTLAQIKEYA DYFFVDDLDY DVETVKKVCK HPDTPLYLEK IKDVWSQLSE FTAENLEASL 

       430        440        450        460        470 
KKLAEENQIP TKNLVHPIRV ALTNKTVGPG LYEIAEVLGK EKTLKRLEKF IQFLKTL 

« Hide

References

[1]"The complete genome sequence of Dictyoglomus thermophilum strain ATCC 35947 / DSM 3960 / H-6-12."
Dodson R.J., Durkin A.S., Wu M., Eisen J., Sutton G.
Submitted (AUG-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 35947 / DSM 3960 / H-6-12.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001146 Genomic DNA. Translation: ACI20166.1.
RefSeqYP_002251003.1. NC_011297.1.

3D structure databases

ProteinModelPortalB5YEP5.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING309799.DICTH_1169.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACI20166; ACI20166; DICTH_1169.
GeneID6945647.
KEGGdth:DICTH_1169.
PATRIC21806966. VBIDicThe33784_1127.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0008.
HOGENOMHOG000252720.
KOK01885.
OMAEIFDMEY.
OrthoDBEOG6DRPF7.

Enzyme and pathway databases

BioCycDTHE309799:GHF9-1157-MONOMER.

Family and domain databases

Gene3D1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
1.10.8.70. 1 hit.
3.40.50.620. 2 hits.
HAMAPMF_00022_B. Glu_tRNA_synth_B.
InterProIPR008925. aa-tRNA-synth_I_codon-bd.
IPR020752. aa-tRNA-synth_I_codon-bd_sub1.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR001412. aa-tRNA-synth_I_CS.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERPTHR10119. PTHR10119. 1 hit.
PfamPF00749. tRNA-synt_1c. 1 hit.
[Graphical view]
PRINTSPR00987. TRNASYNTHGLU.
SUPFAMSSF48163. SSF48163. 1 hit.
TIGRFAMsTIGR00464. gltX_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYE_DICT6
AccessionPrimary (citable) accession number: B5YEP5
Entry history
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: November 25, 2008
Last modified: May 14, 2014
This is version 39 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries