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B5YE54 (B5YE54_DICT6) Unreviewed, UniProtKB/TrEMBL

Last modified May 1, 2013. Version 40. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Valine--tRNA ligase HAMAP-Rule MF_02004
EC=6.1.1.9 HAMAP-Rule MF_02004
Alternative name(s):
Valyl-tRNA synthetase HAMAP-Rule MF_02004
Gene names
Name:valS HAMAP-Rule MF_02004 EMBL ACI19077.1
Ordered Locus Names:DICTH_0962
OrganismDictyoglomus thermophilum (strain ATCC 35947 / DSM 3960 / H-6-12) [Complete proteome] [HAMAP] EMBL ACI19077.1
Taxonomic identifier309799 [NCBI]
Taxonomic lineageBacteria › Dictyoglomi › Dictyoglomales › Dictyoglomaceae › Dictyoglomus ›

Protein attributes

Sequence length881 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the attachment of valine to tRNA(Val). As ValRS can inadvertently accommodate and process structurally similar amino acids such as threonine, to avoid such errors, it has a "posttransfer" editing activity that hydrolyzes mischarged Thr-tRNA(Val) in a tRNA-dependent manner By similarity. HAMAP-Rule MF_02004 SAAS SAAS019499

Catalytic activity

ATP + L-valine + tRNA(Val) = AMP + diphosphate + L-valyl-tRNA(Val). HAMAP-Rule MF_02004 SAAS SAAS019499

Subunit structure

Monomer By similarity. HAMAP-Rule MF_02004 SAAS SAAS019499

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_02004 SAAS SAAS019499.

Domain

The C-terminal coiled-coil domain is crucial for aminoacylation activity By similarity. HAMAP-Rule MF_02004

ValRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated threonine is translocated from the active site to the editing site By similarity. HAMAP-Rule MF_02004

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family. ValS type 1 subfamily. HAMAP-Rule MF_02004

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Coiled coil816 – 88166 By similarity HAMAP-Rule MF_02004
Motif46 – 5611"HIGH" region By similarity HAMAP-Rule MF_02004

Sites

Binding site5281ATP By similarity HAMAP-Rule MF_02004

Sequences

Sequence LengthMass (Da)Tools
B5YE54 [UniParc].

Last modified November 25, 2008. Version 1.
Checksum: 6B07EFD6CD7F89BF

FASTA881103,765
        10         20         30         40         50         60 
MKESMPSVYS FNEVEDKWYK YWLEKDYFHA DVDKNKKPYS IVLPPPNITG SLHMGHALNA 

        70         80         90        100        110        120 
TIQDILIRWR RMQGFNALWI PGTDHAGIAT QMVVERELLK EGKTRWDLGR EKFLERVWQW 

       130        140        150        160        170        180 
KEKYGNTIVE QLKRLGVSCD WKRFRFTMDE VYSRAVIKAF VELYKKGYIY KGERIINWCP 

       190        200        210        220        230        240 
RCKTALSDLE IKYVEENSFL WHIKYPLHNE DGYVIIATAR PETMLGDTAV AVNPEDERYK 

       250        260        270        280        290        300 
NLIGKKVVLP LVGRVIPIIA DEHVDPSFGT GALKVTPAHD ADDFEIGKKH NLEFISVIDE 

       310        320        330        340        350        360 
NGIMNENAGR YKGLNVFECR KKIEEDLEKE GYLLKKEPYI HDLATCDRCG TPIEPLISKQ 

       370        380        390        400        410        420 
WFMRMENIAK PAIEVVEKGE VKFVPDRWKK VYFDWMYNIK DWCLSRQLWW GHRIPAWYCE 

       430        440        450        460        470        480 
DCGHINVEEK QPEKCEKCGS KNLKQDEDVL DTWFSSALWP LGTLGWPEDT EDLNYFYPTS 

       490        500        510        520        530        540 
VLSTARDIIN LWVARMIMMG LEFKGDIPFY YVYVHPTVLT REGKRMSKSK GTGVDPLELI 

       550        560        570        580        590        600 
NKYGADVTRF GLAIQCTEMQ DLRFHEENFE NTKNFTNKIW NAARFVISNL DFNLNYNEVD 

       610        620        630        640        650        660 
LSKHSLSLSD KWILTRLQKV IREVTEHLEN FRFSEYVKTI YNFFWSEFCD WYIELSKPRL 

       670        680        690        700        710        720 
SSTEDPESKL IAQVILWRVL KESMQLLHPV MPFITEEIWQ KLPSTDESIM ISKWPEPNES 

       730        740        750        760        770        780 
LIDEEAERDM IFLMDSIRSI RAIRSEFNIL PNEVINVEFS TPNKYKEVIL QGYSGYFYTL 

       790        800        810        820        830        840 
AKAKLSSTSE KKDLKHVAHK IVEDVNYYVN LEGLIDINKE REKVKKEIDD VSLLIEKIEK 

       850        860        870        880 
RLSDKNFIEK APTEVVEKEK EKLENLKKKF EFLQERYKTL E

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References

[1]"The complete genome sequence of Dictyoglomus thermophilum strain ATCC 35947 / DSM 3960 / H-6-12."
Dodson R.J., Durkin A.S., Wu M., Eisen J., Sutton G.
Submitted (AUG-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 35947 / DSM 3960 / H-6-12.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP001146 Genomic DNA. Translation: ACI19077.1.
RefSeqYP_002250812.1. NC_011297.1.

3D structure databases

ProteinModelPortalB5YE54.
ModBaseSearch...

Protein-protein interaction databases

STRING309799.DICTH_0962.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACI19077; ACI19077; DICTH_0962.
GeneID6945117.
KEGGdth:DICTH_0962.
PATRIC21806562. VBIDicThe33784_0934.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0525.
HOGENOMHOG000020093.
KOK01873.
OMADPLEMIE.
ProtClustDBPRK05729.

Enzyme and pathway databases

BioCycDTHE309799:GHF9-823-MONOMER.

Family and domain databases

Gene3D1.10.287.380. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_02004. Val_tRNA_synth_type1.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR014729. Rossmann-like_a/b/a_fold.
IPR010978. tRNA-bd_arm.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR019499. Val-tRNA_synth_tRNA-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR002303. Valyl-tRNA_ligase.
IPR001876. Znf_RanBP2.
[Graphical view]
PANTHERPTHR11946:SF5. PTHR11946:SF5. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF10458. Val_tRNA-synt_C. 1 hit.
[Graphical view]
PRINTSPR00986. TRNASYNTHVAL.
SMARTSM00547. ZnF_RBZ. 1 hit.
[Graphical view]
SUPFAMSSF46589. tRNA_binding_arm. 1 hit.
SSF47323. tRNAsyn_1a_bind. 1 hit.
SSF50677. ValRS_IleRS_edit. 1 hit.
TIGRFAMsTIGR00422. valS. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameB5YE54_DICT6
AccessionPrimary (citable) accession number: B5YE54
Entry history
Integrated into UniProtKB/TrEMBL: November 25, 2008
Last sequence update: November 25, 2008
Last modified: May 1, 2013
This is version 40 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info