B5YDX5 (TRUA_DICT6) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
tRNA pseudouridine synthase A
EC=5.4.99.12

Alternative name(s):
tRNA pseudouridine(38-40) synthase
tRNA pseudouridylate synthase I
tRNA-uridine isomerase I

Gene names

Name:	truA
Ordered Locus Names:	DICTH_0869

Organism

Dictyoglomus thermophilum (strain ATCC 35947 / DSM 3960 / H-6-12) [Complete proteome] [HAMAP]

Taxonomic identifier

309799 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Dictyoglomi › Dictyoglomia › Dictyoglomales › Dictyoglomaceae › Dictyoglomus › Dictyoglomus thermophilum

Protein attributes

Sequence length	245 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs By similarity. HAMAP-Rule MF_00171
Catalytic activity	tRNA uridine(38-40) = tRNA pseudouridine(38-40). HAMAP-Rule MF_00171
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the tRNA pseudouridine synthase TruA family.

Ontologies

Keywords
Biological process	tRNA processing
Molecular function	Isomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	tRNA pseudouridine synthesis Inferred from electronic annotation. Source: HAMAP
Molecular_function	RNA binding Inferred from electronic annotation. Source: InterPro pseudouridine synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 245	245	tRNA pseudouridine synthase A HAMAP-Rule MF_00171		PRO_1000097738
Sites
	Active site	52	1	Nucleophile By similarity
	Binding site	112	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	B5YDX5 [UniParc]. Last modified November 25, 2008. Version 1. Checksum: 5964C30426FC4FF6	FASTA	245	29,115
	10 20 30 40 50 60 MINWKVELSY IGKDFWGFQK QPGKRTVQGE LEKVLKLLFD EDIKVIGAGR TDAGVHALGQ 70 80 90 100 110 120 VVNFKTKESK NFSCDKLYKV LNKLLPGDIK IKKVEIVDDN FHARYSAKRR WYIYVIYNNE 130 140 150 160 170 180 EKNLFLRDYC WWINKPLDKD LLNLSANLFK GIHDFRNFCV IENYDNTNVE IYESFWYFKE 190 200 210 220 230 240 DLLIYFVSAP FFLRKMVRFI VGSMVEVGLK KKELEDLEKY LKDRKEERFS SPAPASGLYL FKIDY

References

[1]

"The complete genome sequence of Dictyoglomus thermophilum strain ATCC 35947 / DSM 3960 / H-6-12." Dodson R.J., Durkin A.S., Wu M., Eisen J., Sutton G. Submitted (AUG-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 35947 / DSM 3960 / H-6-12.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001146 Genomic DNA. Translation: ACI20111.1.
RefSeq	YP_002250733.1. NC_011297.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	309799.DICTH_0869.
Proteomic databases
PRIDE	B5YDX5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACI20111; ACI20111; DICTH_0869.
GeneID	6945712.
KEGG	dth:DICTH_0869.
PATRIC	21806376. VBIDicThe33784_0854.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0101.
HOGENOM	HOG000248673.
KO	K06173.
OMA	NYCYEYL.
ProtClustDB	CLSK2410133.
Enzyme and pathway databases
BioCyc	DTHE309799:GHF9-1861-MONOMER.
Family and domain databases
Gene3D	3.30.70.580. 1 hit. 3.30.70.660. 1 hit.
HAMAP	MF_00171. TruA.
InterPro	IPR020103. PsdUridine_synth_cat_dom. IPR001406. PsdUridine_synth_TruA. IPR020097. PsdUridine_synth_TruA_a/b_dom. IPR020095. PsdUridine_synth_TruA_C. IPR020094. PsdUridine_synth_TruA_N. [Graphical view]
PANTHER	PTHR11142. PTHR11142. 1 hit.
Pfam	PF01416. PseudoU_synth_1. 2 hits. [Graphical view]
PIRSF	PIRSF001430. tRNA_psdUrid_synth. 1 hit.
SUPFAM	SSF55120. PsdUridine_synth_cat_dom. 1 hit.
TIGRFAMs	TIGR00071. hisT_truA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TRUA_DICT6

Accession

Primary (citable) accession number: B5YDX5

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	November 25, 2008
Last modified:	May 1, 2013
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program