B5YDE1 (PROB_DICT6) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate 5-kinase
EC=2.7.2.11

Alternative name(s):
Gamma-glutamyl kinase
Short name=GK

Gene names

Name:	proB
Ordered Locus Names:	DICTH_0676

Organism

Dictyoglomus thermophilum (strain ATCC 35947 / DSM 3960 / H-6-12) [Complete proteome] [HAMAP]

Taxonomic identifier

309799 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Dictyoglomi › Dictyoglomia › Dictyoglomales › Dictyoglomaceae › Dictyoglomus › Dictyoglomus thermophilum

Protein attributes

Sequence length	371 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5-oxoproline By similarity. HAMAP-Rule MF_00456
Catalytic activity	ATP + L-glutamate = ADP + L-glutamate 5-phosphate. HAMAP-Rule MF_00456
Pathway	Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 1/2. HAMAP-Rule MF_00456
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the glutamate 5-kinase family. Contains 1 PUA domain.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Proline biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	L-proline biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA binding Inferred from electronic annotation. Source: InterPro glutamate 5-kinase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 371	371	Glutamate 5-kinase HAMAP-Rule MF_00456		PRO_1000125226
Regions
	Domain	274 – 352	79	PUA
	Nucleotide binding	169 – 170	2	ATP By similarity
	Nucleotide binding	208 – 214	7	ATP By similarity
Sites
	Binding site	10	1	ATP By similarity
	Binding site	50	1	Substrate By similarity
	Binding site	137	1	Substrate By similarity
	Binding site	149	1	Substrate; via amide nitrogen By similarity

Sequences

	Sequence		Length	Mass (Da)
	B5YDE1 [UniParc]. Last modified November 25, 2008. Version 1. Checksum: 14816C71F82F3AC2	FASTA	371	41,384
	10 20 30 40 50 60 MNDWKRIVVK VGTSSITDER GNPSGEKILS LVRECVKLMR ANKEVVLVSS GAIASGREIM 70 80 90 100 110 120 QKLSKRKDLP AKQALSAVGQ VRLMQYYSQL FSIFKQPIAQ ILLTAEDLRD RKRYINISQT 130 140 150 160 170 180 FETLIEEKVV PIVNENDTVA VEEIKIGDND TLSAKVACAI NADLLVILSD VEGLYSEDPN 190 200 210 220 230 240 LSPNAVLIRD IYEIDEKIEK IAGPGKGTGG MYTKVQAAKI VTEAGIPMIL AKADLENVLE 250 260 270 280 290 300 RIVLKKEKVG TMFYPVERHL NRRKHWMLFM AKPQGKVYID DGAKDALLKK GKSLLPVGVK 310 320 330 340 350 360 KIEGEFVRGD TVSILDLTGE EIGRGITNYD SSELERIKGK NTEEIKNILG EDFYEEVIHR 370 NNLVLINRGD S

References

[1]

"The complete genome sequence of Dictyoglomus thermophilum strain ATCC 35947 / DSM 3960 / H-6-12." Dodson R.J., Durkin A.S., Wu M., Eisen J., Sutton G. Submitted (AUG-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 35947 / DSM 3960 / H-6-12.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001146 Genomic DNA. Translation: ACI19614.1.
RefSeq	YP_002250549.1. NC_011297.1.
3D structure databases
ProteinModelPortal	B5YDE1.
ModBase	Search...
Protein-protein interaction databases
STRING	309799.DICTH_0676.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACI19614; ACI19614; DICTH_0676.
GeneID	6946366.
KEGG	dth:DICTH_0676.
PATRIC	21805996. VBIDicThe33784_0672.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0263.
HOGENOM	HOG000246369.
KO	K00931.
OMA	REPRIAN.
ProtClustDB	CLSK2409853.
Enzyme and pathway databases
BioCyc	DTHE309799:GHF9-1362-MONOMER.
UniPathway	UPA00098; UER00359.
Family and domain databases
Gene3D	3.40.1160.10. 1 hit.
HAMAP	MF_00456. ProB.
InterPro	IPR001048. Asp/Glu/Uridylate_kinase. IPR001057. Glu/AcGlu_kinase. IPR011529. Glu_5kinase. IPR005715. Glu_5kinase/COase_Synthase. IPR002478. PUA. IPR015947. PUA-like_domain. [Graphical view]
Pfam	PF00696. AA_kinase. 1 hit. PF01472. PUA. 1 hit. [Graphical view]
PIRSF	PIRSF000729. GK. 1 hit.
PRINTS	PR00474. GLU5KINASE.
SMART	SM00359. PUA. 1 hit. [Graphical view]
SUPFAM	SSF53633. Aa_kinase. 1 hit. SSF88697. PUA-like. 1 hit.
TIGRFAMs	TIGR01027. proB. 1 hit.
PROSITE	PS00902. GLUTAMATE_5_KINASE. False negative. PS50890. PUA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PROB_DICT6

Accession

Primary (citable) accession number: B5YDE1

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	November 25, 2008
Last modified:	May 1, 2013
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program