B5Y8Q4 (NADE_COPPD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NH(3)-dependent NAD(+) synthetase
EC=6.3.1.5

Gene names

Name:	nadE
Ordered Locus Names:	COPRO5265_0809

Organism

Coprothermobacter proteolyticus (strain ATCC 35245 / DSM 5265 / BT) [Complete proteome] [HAMAP]

Taxonomic identifier

309798 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Thermoanaerobacterales › Thermodesulfobiaceae › Coprothermobacter

Protein attributes

Sequence length	296 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + deamido-NAD+ + NH3 = AMP + diphosphate + NAD+. HAMAP MF_00193
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from deamido-NAD(+) (ammonia route): step 1/1. HAMAP MF_00193
Sequence similarities	Belongs to the NAD synthetase family.

Ontologies

Keywords
Ligand	ATP-binding NAD Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	NAD biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW NAD+ synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: InterPro NAD+ synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 296	296	NH(3)-dependent NAD(+) synthetase HAMAP MF_00193		PRO_1000099017
Regions
	Nucleotide binding	30 – 37	8	ATP By similarity
Sites
	Active site	32	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	B5Y8Q4 [UniParc]. Last modified November 25, 2008. Version 1. Checksum: 56B854BD2B4E1EDE	FASTA	296	34,067
	10 20 30 40 50 60 MNTAMEETQR IEQFLRKALV SERKHGYLIG VSGGLDSAVV LKLLVQAVGK ENVLGLILPD 70 80 90 100 110 120 RDTEKKSTTL ARSLLEQEKV PYKVISMTPL LRHLGVYKDM PLFLLPTRGL KESIVRRFYN 130 140 150 160 170 180 DYTKKLNKPV FFAQWEEPPT QLPYFYEGIA YYRIKHRVRM ATLYYYAEKN DYLLVGCTNL 190 200 210 220 230 240 SERLIGFYVK YGDDVCDVAP IAHLYKTEVR QLSEYLSVPE DIRNRPPSPD LIPGITDEYS 250 260 270 280 290 LGINYETLDQ ILAGLEEGKT AEDLKQLFPA DIVELVINQV KFTQKLEGKP YMLKRA

References

[1]

"The complete genome sequence of Coprothermobacter proteolyticus strain ATCC 5245 / DSM 5265 / BT." Dodson R.J., Durkin A.S., Wu M., Eisen J., Sutton G. Submitted (AUG-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 35245 / DSM 5265 / BT.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001145 Genomic DNA. Translation: ACI18015.1.
RefSeq	YP_002247151.1. NC_011295.1.
3D structure databases
ProteinModelPortal	B5Y8Q4.
ModBase	Search...
Protein-protein interaction databases
STRING	B5Y8Q4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6944506.
GenomeReviews	Gene locus COPRO5265_0809 in contig CP001145_GR.
KEGG	cpo:COPRO5265_0809.
PATRIC	21474452. VBICopPro72829_0764.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG351567.
OMA	MMEREET.
Family and domain databases
HAMAP	MF_00193. NadE. [Tree]
InterPro	IPR022310. NAD/GMP_synthase. IPR003694. NAD_synthase. IPR022926. NH(3)-dep_NAD(+)_synth. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K01916.
Pfam	PF02540. NAD_synthase. 2 hits. [Graphical view]
TIGRFAMs	TIGR00552. NadE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NADE_COPPD

Accession

Primary (citable) accession number: B5Y8Q4

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	November 25, 2008
Last modified:	January 25, 2012
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program