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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

trmB

Organism
Coprothermobacter proteolyticus (strain ATCC 35245 / DSM 5265 / BT)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

Pathwayi: N(7)-methylguanine-tRNA biosynthesis

This protein is involved in the pathway N(7)-methylguanine-tRNA biosynthesis, which is part of tRNA modification.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway N(7)-methylguanine-tRNA biosynthesis and in tRNA modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei28S-adenosyl-L-methionineUniRule annotation1
Binding sitei51S-adenosyl-L-methionineUniRule annotation1
Binding sitei75S-adenosyl-L-methionineUniRule annotation1
Binding sitei134SubstrateUniRule annotation1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00989.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferaseUniRule annotation
Gene namesi
Name:trmBUniRule annotation
Ordered Locus Names:COPRO5265_0563
OrganismiCoprothermobacter proteolyticus (strain ATCC 35245 / DSM 5265 / BT)
Taxonomic identifieri309798 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaThermoanaerobacteralesThermodesulfobiaceaeCoprothermobacter
Proteomesi
  • UP000001732 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003879461 – 319tRNA (guanine-N(7)-)-methyltransferaseAdd BLAST319

Interactioni

Protein-protein interaction databases

STRINGi309798.COPRO5265_0563.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni167 – 170Substrate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CZ1. Bacteria.
COG0220. LUCA.
KOiK03439.
OMAiRTCLLEN.
OrthoDBiPOG091H015P.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01057. tRNA_methyltr_TrmB. 1 hit.
InterProiIPR029063. SAM-dependent_MTases.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B5Y822-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLIKPQSTSS FPLNWDAIFP EKNPLAVEIG FGNGKFLKTL ETTGTNVVGF
60 70 80 90 100
EVSLLSVEKA MKVIDHTKTA LLLMDGIWGL RELFSERSVD ALYINFPLPW
110 120 130 140 150
PHKKHASRRL FTLPKLQIYA SRLVDNAILQ LQTDVKEYAE EAIRNSEESG
160 170 180 190 200
LFSLADYAVR NEVQVGTKYE QKWVSQGKKI YKVVLRKKRH VSVPNYLDKE
210 220 230 240 250
VIMPHAIVHD SHGTLKAGTY RTTFGTIKLW EPFSNNQAML LIPAIVSDDD
260 270 280 290 300
FIGVSLQQRV YISVSPHREG FIVKLDNHAD VFKTENVKSL IWLIANQISN
310
GNIKRINVQP PSKLEYEPA
Length:319
Mass (Da):36,203
Last modified:November 25, 2008 - v1
Checksum:iBA9AB3611373D08A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001145 Genomic DNA. Translation: ACI18005.1.
RefSeqiWP_012544655.1. NC_011295.1.

Genome annotation databases

EnsemblBacteriaiACI18005; ACI18005; COPRO5265_0563.
KEGGicpo:COPRO5265_0563.
PATRICi21473960. VBICopPro72829_0530.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001145 Genomic DNA. Translation: ACI18005.1.
RefSeqiWP_012544655.1. NC_011295.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi309798.COPRO5265_0563.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACI18005; ACI18005; COPRO5265_0563.
KEGGicpo:COPRO5265_0563.
PATRICi21473960. VBICopPro72829_0530.

Phylogenomic databases

eggNOGiENOG4105CZ1. Bacteria.
COG0220. LUCA.
KOiK03439.
OMAiRTCLLEN.
OrthoDBiPOG091H015P.

Enzyme and pathway databases

UniPathwayiUPA00989.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01057. tRNA_methyltr_TrmB. 1 hit.
InterProiIPR029063. SAM-dependent_MTases.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTRMB_COPPD
AccessioniPrimary (citable) accession number: B5Y822
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 3, 2009
Last sequence update: November 25, 2008
Last modified: November 2, 2016
This is version 42 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.