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B5Y822 (TRMB_COPPD) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 32. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA (guanine-N(7)-)-methyltransferase

EC=2.1.1.33
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene names
Name:trmB
Ordered Locus Names:COPRO5265_0563
OrganismCoprothermobacter proteolyticus (strain ATCC 35245 / DSM 5265 / BT) [Complete proteome] [HAMAP]
Taxonomic identifier309798 [NCBI]
Taxonomic lineageBacteriaFirmicutesClostridiaThermoanaerobacteralesThermodesulfobiaceaeCoprothermobacter

Protein attributes

Sequence length319 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_01057

Catalytic activity

S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_01057

Pathway

tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP-Rule MF_01057

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.

Ontologies

Keywords
   Biological processtRNA processing
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Molecular_functiontRNA (guanine-N7-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 319319tRNA (guanine-N(7)-)-methyltransferase HAMAP-Rule MF_01057
PRO_0000387946

Regions

Region167 – 1704Substrate binding By similarity

Sites

Binding site281S-adenosyl-L-methionine By similarity
Binding site511S-adenosyl-L-methionine By similarity
Binding site751S-adenosyl-L-methionine By similarity
Binding site1341Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
B5Y822 [UniParc].

Last modified November 25, 2008. Version 1.
Checksum: BA9AB3611373D08A

FASTA31936,203
        10         20         30         40         50         60 
MLIKPQSTSS FPLNWDAIFP EKNPLAVEIG FGNGKFLKTL ETTGTNVVGF EVSLLSVEKA 

        70         80         90        100        110        120 
MKVIDHTKTA LLLMDGIWGL RELFSERSVD ALYINFPLPW PHKKHASRRL FTLPKLQIYA 

       130        140        150        160        170        180 
SRLVDNAILQ LQTDVKEYAE EAIRNSEESG LFSLADYAVR NEVQVGTKYE QKWVSQGKKI 

       190        200        210        220        230        240 
YKVVLRKKRH VSVPNYLDKE VIMPHAIVHD SHGTLKAGTY RTTFGTIKLW EPFSNNQAML 

       250        260        270        280        290        300 
LIPAIVSDDD FIGVSLQQRV YISVSPHREG FIVKLDNHAD VFKTENVKSL IWLIANQISN 

       310 
GNIKRINVQP PSKLEYEPA 

« Hide

References

[1]"The complete genome sequence of Coprothermobacter proteolyticus strain ATCC 5245 / DSM 5265 / BT."
Dodson R.J., Durkin A.S., Wu M., Eisen J., Sutton G.
Submitted (AUG-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 35245 / DSM 5265 / BT.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001145 Genomic DNA. Translation: ACI18005.1.
RefSeqYP_002246919.1. NC_011295.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING309798.COPRO5265_0563.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACI18005; ACI18005; COPRO5265_0563.
GeneID6944137.
KEGGcpo:COPRO5265_0563.
PATRIC21473960. VBICopPro72829_0530.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0220.
KOK03439.
OrthoDBEOG6K6VBC.

Enzyme and pathway databases

BioCycCPRO309798:GH7M-558-MONOMER.
UniPathwayUPA00989.

Family and domain databases

HAMAPMF_01057. tRNA_methyltr_TrmB.
InterProIPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamPF02390. Methyltransf_4. 1 hit.
[Graphical view]
PROSITEPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRMB_COPPD
AccessionPrimary (citable) accession number: B5Y822
Entry history
Integrated into UniProtKB/Swiss-Prot: November 3, 2009
Last sequence update: November 25, 2008
Last modified: April 16, 2014
This is version 32 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways