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B5Y7R0 (SYE_COPPD) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 37. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutamate--tRNA ligase

EC=6.1.1.17
Alternative name(s):
Glutamyl-tRNA synthetase
Short name=GluRS
Gene names
Name:gltX
Ordered Locus Names:COPRO5265_0442
OrganismCoprothermobacter proteolyticus (strain ATCC 35245 / DSM 5265 / BT) [Complete proteome] [HAMAP]
Taxonomic identifier309798 [NCBI]
Taxonomic lineageBacteriaFirmicutesClostridiaThermoanaerobacteralesThermodesulfobiaceaeCoprothermobacter

Protein attributes

Sequence length467 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity. HAMAP-Rule MF_00022

Catalytic activity

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP-Rule MF_00022

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00022

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00022.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processglutamyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

glutamate-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

tRNA binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 467467Glutamate--tRNA ligase HAMAP-Rule MF_00022
PRO_0000367653

Regions

Motif15 – 2511"HIGH" region HAMAP-Rule MF_00022
Motif249 – 2535"KMSKS" region HAMAP-Rule MF_00022

Sites

Binding site2521ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
B5Y7R0 [UniParc].

Last modified November 25, 2008. Version 1.
Checksum: D9995F970A95DB0E

FASTA46753,985
        10         20         30         40         50         60 
MSVNDSTHIK TRFAPSPTGY LHVGGARTAL FNYLFAKKFG GTFLLRIEDT DPERSKPEYS 

        70         80         90        100        110        120 
EQILISMKWL GLDWDEGPYH QSERMHIYQQ YTQKLLEEGK AYRCFCTTEE LEQMREQQRQ 

       130        140        150        160        170        180 
QGLPTRYDGR CSRLTQEEIE ERLNKGMPFA VRLKVPQDRG IIAWDDMVKG HIEINSSELD 

       190        200        210        220        230        240 
DFILVRSDGT PTYNFAVVID DHTMGVTHVL RGEDHIPNTP KQILIYEALG WETPEFGHVP 

       250        260        270        280        290        300 
MILGKDKTKL SKRHGAVGVE AYRDEGFLPE ALFNFLALLG ASYDPDREVY TKQELIDLFD 

       310        320        330        340        350        360 
PKKIGLHPAV FDPDKLYYIN REHMKMLPPE ELLDRIRPFA EAKGFQIEPY HLRLIPLLVE 

       370        380        390        400        410        420 
RMRTLKDFVE LADYIFTDDF TVDEKAQELL AKDLPLGGLA EQLDATVWDA DHIEAVLRQY 

       430        440        450        460 
AQDKGIKPRD YFPFIRAVIS GKSVGPSLFH LMEAMPKDMV LRRLRKS 

« Hide

References

[1]"The complete genome sequence of Coprothermobacter proteolyticus strain ATCC 5245 / DSM 5265 / BT."
Dodson R.J., Durkin A.S., Wu M., Eisen J., Sutton G.
Submitted (AUG-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 35245 / DSM 5265 / BT.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001145 Genomic DNA. Translation: ACI16900.1.
RefSeqYP_002246807.1. NC_011295.1.

3D structure databases

ProteinModelPortalB5Y7R0.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING309798.COPRO5265_0442.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACI16900; ACI16900; COPRO5265_0442.
GeneID6944687.
KEGGcpo:COPRO5265_0442.
PATRIC21473736. VBICopPro72829_0423.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0008.
HOGENOMHOG000252720.
KOK01885.
OMARANQGKF.
OrthoDBEOG6DRPF7.

Enzyme and pathway databases

BioCycCPRO309798:GH7M-437-MONOMER.

Family and domain databases

Gene3D1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
1.10.8.70. 1 hit.
3.40.50.620. 2 hits.
HAMAPMF_00022_B. Glu_tRNA_synth_B.
InterProIPR008925. aa-tRNA-synth_I_codon-bd.
IPR020752. aa-tRNA-synth_I_codon-bd_sub1.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR001412. aa-tRNA-synth_I_CS.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERPTHR10119. PTHR10119. 1 hit.
PfamPF00749. tRNA-synt_1c. 1 hit.
[Graphical view]
PRINTSPR00987. TRNASYNTHGLU.
SUPFAMSSF48163. SSF48163. 1 hit.
TIGRFAMsTIGR00464. gltX_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYE_COPPD
AccessionPrimary (citable) accession number: B5Y7R0
Entry history
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: November 25, 2008
Last modified: February 19, 2014
This is version 37 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries