Reviewed, UniProtKB/Swiss-Prot B5Y345 (SYK3_KLEP3)

Names and origin

Protein names

Recommended name:
    Putative lysyl-tRNA synthetase
    EC=6.1.1.6
Alternative name(s):
    Lysine--tRNA ligase
      Short name=LysRS
    GX

Gene names

Name:	poxA
Ordered Locus Names:	KPK_5114

Organism

Klebsiella pneumoniae (strain 342) [Complete proteome] [HAMAP]

Taxonomic identifier

507522 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Klebsiella

Protein attributes

Sequence length	325 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Could be a lysyl-tRNA synthetase By similarity.
Catalytic activity	ATP + L-lysine + tRNA(Lys) = AMP + diphosphate + L-lysyl-tRNA(Lys). HAMAP MF_00174
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	lysyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP lysine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 325	325	Putative lysyl-tRNA synthetase HAMAP MF_00174		PRO_1000097902

Sequences

	Sequence		Length	Mass (Da)
	B5Y345-1 [UniParc]. Last modified November 25, 2008. Version 1. Checksum: A9D9E652E42EFD26	FASTA	325	36,881
	10 20 30 40 50 60 MSETATWQPS APIPNLLKRA AVMAEIRRFF TDRGVLEVET PCMSQATVTD IHLFPFETRF 70 80 90 100 110 120 VGPGHSQGLN LYLMTSPEYH MKRLLAAGCG PVFQLCRSFR NEEMGRHHNP EFTMLEWYRP 130 140 150 160 170 180 CYDMYRLINE VDDLLQQVLE CQPAESLSYQ QAFQRHLDID PLSADKTQLR EVAAKLDLSN 190 200 210 220 230 240 IADTEEDRDT LLQLLFTMGV EPHIGKDRPT FIYHFPATQA SLAQISPEDH RVAERFEVYY 250 260 270 280 290 300 KGIELANGFH ELTDAREQRL RFEQDNRKRA ARGLPQQPID NNLLAALEAG LPDCSGVALG 310 320 VDRVVMLALG AESIGEVIAF TVDRA

References

[1]

"Complete genome sequence of the N2-fixing broad host range endophyte Klebsiella pneumoniae 342 and virulence predictions verified in mice." Fouts D.E., Tyler H.L., DeBoy R.T., Daugherty S., Ren Q., Badger J.H., Durkin A.S., Huot H., Shrivastava S., Kothari S., Dodson R.J., Mohamoud Y., Khouri H., Roesch L.F.W., Krogfelt K.A., Struve C., Triplett E.W., Methe B.A. PLoS Genet. 4:E1000141-E1000141(2008) [PubMed: 18654632] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000964 Genomic DNA. Translation: ACI11575.1.
RefSeq	YP_002240886.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	6935980.
GenomeReviews	Gene locus KPK_5114 in contig CP000964_GR.
KEGG	kpe:KPK_5114.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	VFRNEEA.
Family and domain databases
HAMAP	MF_00174. [Tree]
InterPro	IPR004364. aa-tRNA-synt_II. IPR018150. aa-tRNA-synt_II-like. IPR006195. aa-tRNA-synth_II_cons-reg. IPR004525. Lys-tRNA-synth-rel. IPR018149. Lys-tRNA-synth_II_C. [Graphical view]
PANTHER	PTHR22594. aa-tRNA-synt_II. 1 hit.
Pfam	PF00152. tRNA-synt_2. 1 hit. [Graphical view]
PRINTS	PR00982. TRNASYNTHLYS.
TIGRFAMs	TIGR00462. genX. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYK3_KLEP3

Accession

Primary (citable) accession number: B5Y345

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	November 25, 2008
Last modified:	November 3, 2009
This is version 9 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)