B5Y1W9 (AROD_KLEP3) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3-dehydroquinate dehydratase
Short name=3-dehydroquinase
EC=4.2.1.10

Alternative name(s):
Type I DHQase

Gene names

Name:	aroD
Ordered Locus Names:	KPK_4669

Organism

Klebsiella pneumoniae (strain 342) [Complete proteome] [HAMAP]

Taxonomic identifier

507522 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Klebsiella

Protein attributes

Sequence length	251 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	3-dehydroquinate = 3-dehydroshikimate + H2O. HAMAP MF_00214
Pathway	Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 3/7. HAMAP MF_00214
Subunit structure	Homodimer By similarity. HAMAP MF_00214
Sequence similarities	Belongs to the type-I 3-dehydroquinase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis
Ligand	Schiff base
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	aromatic amino acid family biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	3-dehydroquinate dehydratase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 251	251	3-dehydroquinate dehydratase HAMAP MF_00214		PRO_1000099909
Sites
	Active site	144	1	Proton acceptor By similarity
	Active site	171	1	Schiff-base intermediate with substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	B5Y1W9 [UniParc]. Last modified November 25, 2008. Version 1. Checksum: 5BAA6A830CDAF979	FASTA	251	27,279
	10 20 30 40 50 60 MTNAVTVKNI TFQEGETLIC VPLIGKTLDE ILGNAHGLVD AGADIIEWRV DHFTQVRDMT 70 80 90 100 110 120 QVMAALAEIR GALKALPLLF TFRSKKEGGE TEISDEAYFA LNREAARSGL VDVIDIELFN 130 140 150 160 170 180 DEAQIHALVD DAHAAGVKVI MSNHDFHKTP AQEDIIYRLR RMQDLGADLP KIAVMPQSPQ 190 200 210 220 230 240 DVLTLLAATL TMKEKYATRP LITMSMGKSG GVSRVTGRLF GSAMTFGTVG QASAPGQIAI 250 AKLREVMDIL S

References

[1]

"Complete genome sequence of the N2-fixing broad host range endophyte Klebsiella pneumoniae 342 and virulence predictions verified in mice." Fouts D.E., Tyler H.L., DeBoy R.T., Daugherty S., Ren Q., Badger J.H., Durkin A.S., Huot H., Shrivastava S., Kothari S., Dodson R.J., Mohamoud Y., Khouri H., Roesch L.F.W., Krogfelt K.A., Struve C., Triplett E.W., Methe B.A. PLoS Genet. 4:E1000141-E1000141(2008) [PubMed: 18654632] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 342.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000964 Genomic DNA. Translation: ACI07351.1.
RefSeq	YP_002240460.1. NC_011283.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	B5Y1W9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6935785.
GenomeReviews	Gene locus KPK_4669 in contig CP000964_GR.
KEGG	kpe:KPK_4669.
PATRIC	20442487. VBIKlePne121904_4585.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG732926.
OMA	IAKIAVM.
ProtClustDB	CLSK2400569.
Family and domain databases
HAMAP	MF_00214. AroD. [Tree]
InterPro	IPR018508. 3-dehydroquinate_DH_AS. IPR013785. Aldolase_TIM. IPR001381. DHquinase_I. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
KO	K03785.
Pfam	PF01487. DHquinase_I. 1 hit. [Graphical view]
TIGRFAMs	TIGR01093. AroD. 1 hit.
PROSITE	PS01028. DEHYDROQUINASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AROD_KLEP3

Accession

Primary (citable) accession number: B5Y1W9

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	November 25, 2008
Last modified:	January 25, 2012
This is version 23 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program