Reviewed, UniProtKB/Swiss-Prot B5Y008 (BETA_KLEP3)

Names and origin

Protein names

Recommended name:
    Choline dehydrogenase
      Short name=CHD
      Short name=CDH
    EC=1.1.99.1

Gene names

Name:	betA
Ordered Locus Names:	KPK_3995

Organism

Klebsiella pneumoniae (strain 342) [Complete proteome] [HAMAP]

Taxonomic identifier

507522 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Klebsiella

Protein attributes

Sequence length	554 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Can catalyze the oxidation of choline to betaine aldehyde and betaine aldehyde to glycine betaine By similarity.
Catalytic activity	Choline + acceptor = betaine aldehyde + reduced acceptor. HAMAP MF_00750
Cofactor	FAD By similarity.
Pathway	Amine and polyamine biosynthesis; betaine biosynthesis via choline pathway; betaine aldehyde from choline (cytochrome c reductase route): step 1/1. HAMAP MF_00750
Sequence similarities	Belongs to the GMC oxidoreductase family.

Ontologies

Keywords
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycine betaine biosynthetic process from choline Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	FAD binding Inferred from electronic annotation. Source: InterPro choline dehydrogenase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 554	554	Choline dehydrogenase HAMAP MF_00750		PRO_1000133333
Regions
	Nucleotide binding	4 – 33	30	FAD Probable
Sites
	Active site	473	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	B5Y008-1 [UniParc]. Last modified November 25, 2008. Version 1. Checksum: 54C22482DD100D84	FASTA	554	61,431
	10 20 30 40 50 60 MQFDYIIIGA GSAGNVLATR LTEDPNTTVL LLEAGGPDYR FDFRTQMPAA LAYPLQGKRY 70 80 90 100 110 120 NWAYETEPEP YMNHRRMECG RGKGLGGSSL INGMCYIRGN AMDLDNWAKE PGLEHWSYLD 130 140 150 160 170 180 CLPYYRKAET RDIGPNDYHG GDGPVSVTTP KPGNNPLFEA MVTAGVQAGY PRTDDLNGYQ 190 200 210 220 230 240 QEGFGPMDRT VTPQGRRAST ARGYLDQARG RPNLTIRTHA LTDHIIFAGK RAVGVEWLEG 250 260 270 280 290 300 ESTIPSKATA NKEVLLCAGA IASPQILQRS GVGNPELLRQ FDIPVVHDLP GVGENLQDHL 310 320 330 340 350 360 EMYLQYECKE PVSLYPALQW WNQPKIGAEW LFGGTGIGAS NQFEAGGFIR SRAEFAWPNI 370 380 390 400 410 420 QYHFLPVAIN YNGSNAVKEH GFQCHVGSMR SPSRGHVRLK SRDPHAHPAI LFNYMSHEQD 430 440 450 460 470 480 WQEFRDAIRI TREIMNQPAL DKYRGREISP GTECQSDAEL DEFVRNHAET AFHPCGTCKM 490 500 510 520 530 540 GYDEMAVVDG EGRVHGLEGV RVVDASIMPQ IITGNLNATT IMIGEKMADA IRGRQPLPRS 550 TAAYYVAGGA PVRR

References

[1]

"Complete genome sequence of the N2-fixing broad host range endophyte Klebsiella pneumoniae 342 and virulence predictions verified in mice." Fouts D.E., Tyler H.L., DeBoy R.T., Daugherty S., Ren Q., Badger J.H., Durkin A.S., Huot H., Shrivastava S., Kothari S., Dodson R.J., Mohamoud Y., Khouri H., Roesch L.F.W., Krogfelt K.A., Struve C., Triplett E.W., Methe B.A. PLoS Genet. 4:E1000141-E1000141(2008) [PubMed: 18654632] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000964 Genomic DNA. Translation: ACI07067.1.
RefSeq	YP_002239799.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	6939067.
GenomeReviews	Gene locus KPK_3995 in contig CP000964_GR.
KEGG	kpe:KPK_3995.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	PNLQYHF.
Family and domain databases
HAMAP	MF_00750. [Tree]
InterPro	IPR011533. Choline_dehydrogenase. IPR012132. GMC_OxRdtase. IPR000172. GMC_OxRdtase_N. IPR007867. GMC_OxRtase_C. [Graphical view]
Pfam	PF05199. GMC_oxred_C. 1 hit. PF00732. GMC_oxred_N. 1 hit. [Graphical view]
PIRSF	PIRSF000137. Alcohol_oxidase. 1 hit.
TIGRFAMs	TIGR01810. betA. 1 hit.
PROSITE	PS00623. GMC_OXRED_1. 1 hit. PS00624. GMC_OXRED_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BETA_KLEP3

Accession

Primary (citable) accession number: B5Y008

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	November 25, 2008
Last modified:	November 3, 2009
This is version 11 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)