Reviewed, UniProtKB/Swiss-Prot B5XZL2 (ASNA_KLEP3)

Names and origin

Protein names

Recommended name:
    Aspartate--ammonia ligase
    EC=6.3.1.1
Alternative name(s):
    Asparagine synthetase A

Gene names

Name:	asnA
Ordered Locus Names:	KPK_5532

Organism

Klebsiella pneumoniae (strain 342) [Complete proteome] [HAMAP]

Taxonomic identifier

507522 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Klebsiella

Protein attributes

Sequence length	330 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-aspartate + NH3 = AMP + diphosphate + L-asparagine. HAMAP MF_00555
Pathway	Amino-acid biosynthesis; L-asparagine biosynthesis; L-asparagine from L-aspartate (ammonia route): step 1/1. HAMAP MF_00555
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. AsnA subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Asparagine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	asparagine biosynthetic process Inferred from electronic annotation. Source: HAMAP tRNA aminoacylation for protein translation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP aminoacyl-tRNA ligase activity Inferred from electronic annotation. Source: InterPro aspartate-ammonia ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 330	330	Aspartate--ammonia ligase HAMAP MF_00555		PRO_1000129120

Sequences

	Sequence		Length	Mass (Da)
	B5XZL2-1 [UniParc]. Last modified November 25, 2008. Version 1. Checksum: 71285EC32903517D	FASTA	330	36,826
	10 20 30 40 50 60 MKTAYIAKQR QISFVKSHFS RQLEEKLGLI EVQAPILSRV GDGTQDNLSG CEKAVQVKVK 70 80 90 100 110 120 TLPDAQFEVV HSLAKWKRQT LGQHDFSAGE GLYTHMKALR PDEDRLTPIH SVYVDQWDWE 130 140 150 160 170 180 RVMGDEERHV GTLKATVEAI YAGIKATELA VSQEFGLKPF LPEQIHFVHS QELLSRYPDL 190 200 210 220 230 240 DAKGRERAIA KELGAVFLIG IGGKLSNGKR HDVRAPDYDD WSTEVSEGFA GLNGDILVWN 250 260 270 280 290 300 PVLEDAFEIS SMGIRVDAEA LKRQLAVTGD EDRLQLEWHQ ALLRGEMPQT IGGGIGQSRL 310 320 330 TMLLLQLDHI GQVQCGVWPA QVRESVSALL

References

[1]

"Complete genome sequence of the N2-fixing broad host range endophyte Klebsiella pneumoniae 342 and virulence predictions verified in mice." Fouts D.E., Tyler H.L., DeBoy R.T., Daugherty S., Ren Q., Badger J.H., Durkin A.S., Huot H., Shrivastava S., Kothari S., Dodson R.J., Mohamoud Y., Khouri H., Roesch L.F.W., Krogfelt K.A., Struve C., Triplett E.W., Methe B.A. PLoS Genet. 4:E1000141-E1000141(2008) [PubMed: 18654632] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000964 Genomic DNA. Translation: ACI09724.1.
RefSeq	YP_002241275.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	6938494.
GenomeReviews	Gene locus KPK_5532 in contig CP000964_GR.
KEGG	kpe:KPK_5532.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	LNDNLNG.
Family and domain databases
HAMAP	MF_00555. [Tree]
InterPro	IPR006195. aa-tRNA-synth_II_cons-reg. IPR004618. AsnA. [Graphical view]
Pfam	PF03590. AsnA. 1 hit. [Graphical view]
PIRSF	PIRSF001555. Asp_ammon_ligase. 1 hit.
TIGRFAMs	TIGR00669. asnA. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ASNA_KLEP3

Accession

Primary (citable) accession number: B5XZL2

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	November 25, 2008
Last modified:	November 3, 2009
This is version 11 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)