B5XZ80 (HUTU_KLEP3) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Urocanate hydratase
Short name=Urocanase
EC=4.2.1.49

Alternative name(s):
Imidazolonepropionate hydrolase

Gene names

Name:	hutU
Ordered Locus Names:	KPK_3778

Organism

Klebsiella pneumoniae (strain 342) [Complete proteome] [HAMAP]

Taxonomic identifier

507522 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Klebsiella › Klebsiella pneumoniae

Protein attributes

Sequence length	562 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	3-(5-oxo-4,5-dihydro-3H-imidazol-4-yl)propanoate = urocanate + H2O. HAMAP-Rule MF_00577
Cofactor	Binds 1 NAD per subunit By similarity. HAMAP-Rule MF_00577
Pathway	Amino-acid degradation; L-histidine degradation into L-glutamate; N-formimidoyl-L-glutamate from L-histidine: step 2/3. HAMAP-Rule MF_00577
Subcellular location	Cytoplasm By similarity HAMAP-Rule MF_00577.
Sequence similarities	Belongs to the urocanase family.

Ontologies

Keywords
Biological process	Histidine metabolism
Cellular component	Cytoplasm
Ligand	NAD
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	histidine catabolic process to glutamate and formamide Inferred from electronic annotation. Source: UniProtKB-UniPathway histidine catabolic process to glutamate and formate Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	urocanate hydratase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 562	562	Urocanate hydratase HAMAP-Rule MF_00577		PRO_1000129565
Sites
	Active site	410	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	B5XZ80 [UniParc]. Last modified November 25, 2008. Version 1. Checksum: 8F984F14F7AC1AC3	FASTA	562	61,562
	10 20 30 40 50 60 MSQSKYRQLD VRAPRGTTLT ARSWLTEAPL RMLMNNLDPD VAENPHELVV YGGIGRAARN 70 80 90 100 110 120 WECYDAIVKA LKNLESDETL LVQSGKPVGV FKTHENSPRV LIANSNLVPH WATWEHFNEL 130 140 150 160 170 180 DAKGLAMYGQ MTAGSWIYIG SQGIVQGTYE TFVEAGRQHY QGSLKGRWVL TAGLGGMGGA 190 200 210 220 230 240 QPLAATLAGA CSLNIECQQS RIDFRLRTRY VDEQATSLDD ALARIKKYTA EGRAISIALC 250 260 270 280 290 300 GNAADIVPEL VKRGVRPDMV TDQTSAHDPL HGYLPKGWSW EEYQQKAESD PQGTILAAKR 310 320 330 340 350 360 SMADHVQAML AFNEMGVPTF DYGNNIRQMA QEMGVSNAFD FPGFVPAYIR PLFCRGIGPF 370 380 390 400 410 420 RWVALSGDPQ DIYKTDAKVK EIIKDDKHLH HWLDMARERI SFQGLPARIC WVGLEWRQKL 430 440 450 460 470 480 GLAFNEMVRS GEVSAPIVIG RDHLDSGSVA SPNRETEAMR DGSDAVSDWP LLNALLNTAS 490 500 510 520 530 540 GATWVSLHHG GGVGMGFSQH SGMVIVCDGT DEAAARIARV LRNDPATGVM RHADAGYDIA 550 560 IECAAEQGLN LPMVAATQGN AK

References

[1]

"Complete genome sequence of the N2-fixing broad host range endophyte Klebsiella pneumoniae 342 and virulence predictions verified in mice." Fouts D.E., Tyler H.L., DeBoy R.T., Daugherty S., Ren Q., Badger J.H., Durkin A.S., Huot H., Shrivastava S., Kothari S., Dodson R.J., Mohamoud Y., Khouri H., Roesch L.F.W., Krogfelt K.A., Struve C., Triplett E.W., Methe B.A. PLoS Genet. 4:E1000141-E1000141(2008) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 342.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000964 Genomic DNA. Translation: ACI09126.1.
RefSeq	YP_002239597.1. NC_011283.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	507522.KPK_3778.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACI09126; ACI09126; KPK_3778.
GeneID	6935033.
KEGG	kpe:KPK_3778.
PATRIC	20440839. VBIKlePne121904_3787.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2987.
HOGENOM	HOG000237606.
KO	K01712.
OMA	IRQMAFE.
ProtClustDB	PRK05414.
Enzyme and pathway databases
BioCyc	KPNE507522:GI0B-3779-MONOMER.
UniPathway	UPA00379; UER00550.
Family and domain databases
HAMAP	MF_00577. HutU.
InterPro	IPR023637. Urocanase. IPR023636. Urocanase_CS. [Graphical view]
PANTHER	PTHR32312. PTHR32312. 1 hit.
Pfam	PF01175. Urocanase. 1 hit. [Graphical view]
PIRSF	PIRSF001423. Urocanate_hydrat. 1 hit.
SUPFAM	SSF111326. Urocanase. 1 hit.
TIGRFAMs	TIGR01228. hutU. 1 hit.
PROSITE	PS01233. UROCANASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HUTU_KLEP3

Accession

Primary (citable) accession number: B5XZ80

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	November 25, 2008
Last modified:	May 29, 2013
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program