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B5XZ80 (HUTU_KLEP3) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 37. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Urocanate hydratase

Short name=Urocanase
EC=4.2.1.49
Alternative name(s):
Imidazolonepropionate hydrolase
Gene names
Name:hutU
Ordered Locus Names:KPK_3778
OrganismKlebsiella pneumoniae (strain 342) [Complete proteome] [HAMAP]
Taxonomic identifier507522 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeKlebsiella

Protein attributes

Sequence length562 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

3-(5-oxo-4,5-dihydro-3H-imidazol-4-yl)propanoate = urocanate + H2O. HAMAP-Rule MF_00577

Cofactor

Binds 1 NAD per subunit By similarity. HAMAP-Rule MF_00577

Pathway

Amino-acid degradation; L-histidine degradation into L-glutamate; N-formimidoyl-L-glutamate from L-histidine: step 2/3. HAMAP-Rule MF_00577

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00577.

Sequence similarities

Belongs to the urocanase family.

Ontologies

Keywords
   Biological processHistidine metabolism
   Cellular componentCytoplasm
   LigandNAD
   Molecular functionLyase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processhistidine catabolic process to glutamate and formamide

Inferred from electronic annotation. Source: UniProtKB-UniPathway

histidine catabolic process to glutamate and formate

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionurocanate hydratase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 562562Urocanate hydratase HAMAP-Rule MF_00577
PRO_1000129565

Sites

Active site4101 By similarity

Sequences

Sequence LengthMass (Da)Tools
B5XZ80 [UniParc].

Last modified November 25, 2008. Version 1.
Checksum: 8F984F14F7AC1AC3

FASTA56261,562
        10         20         30         40         50         60 
MSQSKYRQLD VRAPRGTTLT ARSWLTEAPL RMLMNNLDPD VAENPHELVV YGGIGRAARN 

        70         80         90        100        110        120 
WECYDAIVKA LKNLESDETL LVQSGKPVGV FKTHENSPRV LIANSNLVPH WATWEHFNEL 

       130        140        150        160        170        180 
DAKGLAMYGQ MTAGSWIYIG SQGIVQGTYE TFVEAGRQHY QGSLKGRWVL TAGLGGMGGA 

       190        200        210        220        230        240 
QPLAATLAGA CSLNIECQQS RIDFRLRTRY VDEQATSLDD ALARIKKYTA EGRAISIALC 

       250        260        270        280        290        300 
GNAADIVPEL VKRGVRPDMV TDQTSAHDPL HGYLPKGWSW EEYQQKAESD PQGTILAAKR 

       310        320        330        340        350        360 
SMADHVQAML AFNEMGVPTF DYGNNIRQMA QEMGVSNAFD FPGFVPAYIR PLFCRGIGPF 

       370        380        390        400        410        420 
RWVALSGDPQ DIYKTDAKVK EIIKDDKHLH HWLDMARERI SFQGLPARIC WVGLEWRQKL 

       430        440        450        460        470        480 
GLAFNEMVRS GEVSAPIVIG RDHLDSGSVA SPNRETEAMR DGSDAVSDWP LLNALLNTAS 

       490        500        510        520        530        540 
GATWVSLHHG GGVGMGFSQH SGMVIVCDGT DEAAARIARV LRNDPATGVM RHADAGYDIA 

       550        560 
IECAAEQGLN LPMVAATQGN AK 

« Hide

References

[1]"Complete genome sequence of the N2-fixing broad host range endophyte Klebsiella pneumoniae 342 and virulence predictions verified in mice."
Fouts D.E., Tyler H.L., DeBoy R.T., Daugherty S., Ren Q., Badger J.H., Durkin A.S., Huot H., Shrivastava S., Kothari S., Dodson R.J., Mohamoud Y., Khouri H., Roesch L.F.W., Krogfelt K.A., Struve C., Triplett E.W., Methe B.A.
PLoS Genet. 4:E1000141-E1000141(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 342.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000964 Genomic DNA. Translation: ACI09126.1.
RefSeqYP_002239597.1. NC_011283.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING507522.KPK_3778.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACI09126; ACI09126; KPK_3778.
GeneID6935033.
KEGGkpe:KPK_3778.
PATRIC20440839. VBIKlePne121904_3787.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG2987.
HOGENOMHOG000237606.
KOK01712.
OMAHEWAPRV.
OrthoDBEOG62K1TR.
ProtClustDBPRK05414.

Enzyme and pathway databases

BioCycKPNE507522:GI0B-3779-MONOMER.
UniPathwayUPA00379; UER00550.

Family and domain databases

HAMAPMF_00577. HutU.
InterProIPR023637. Urocanase.
IPR023636. Urocanase_CS.
[Graphical view]
PANTHERPTHR32312. PTHR32312. 1 hit.
PfamPF01175. Urocanase. 1 hit.
[Graphical view]
PIRSFPIRSF001423. Urocanate_hydrat. 1 hit.
SUPFAMSSF111326. SSF111326. 1 hit.
TIGRFAMsTIGR01228. hutU. 1 hit.
PROSITEPS01233. UROCANASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameHUTU_KLEP3
AccessionPrimary (citable) accession number: B5XZ80
Entry history
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: November 25, 2008
Last modified: February 19, 2014
This is version 37 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways