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Protein

Urocanate hydratase

Gene

hutU

Organism
Klebsiella pneumoniae (strain 342)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the conversion of urocanate to 4-imidazolone-5-propionate.UniRule annotation

Catalytic activityi

3-(5-oxo-4,5-dihydro-3H-imidazol-4-yl)propanoate = urocanate + H2O.UniRule annotation

Cofactori

NAD+UniRule annotationNote: Binds 1 NAD+ per subunit.UniRule annotation

Pathwayi: L-histidine degradation into L-glutamate

This protein is involved in step 2 of the subpathway that synthesizes N-formimidoyl-L-glutamate from L-histidine.UniRule annotation
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Histidine ammonia-lyase (hutH)
  2. Urocanate hydratase (hutU)
  3. Imidazolonepropionase (hutI)
This subpathway is part of the pathway L-histidine degradation into L-glutamate, which is itself part of Amino-acid degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes N-formimidoyl-L-glutamate from L-histidine, the pathway L-histidine degradation into L-glutamate and in Amino-acid degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei130NADUniRule annotation1
Binding sitei196NADUniRule annotation1
Binding sitei201NADUniRule annotation1
Binding sitei322NAD; via carbonyl oxygenUniRule annotation1
Active sitei410UniRule annotation1
Binding sitei492NAD; via carbonyl oxygenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi52 – 53NADUniRule annotation2
Nucleotide bindingi176 – 178NADUniRule annotation3
Nucleotide bindingi242 – 243NADUniRule annotation2
Nucleotide bindingi263 – 267NADUniRule annotation5
Nucleotide bindingi273 – 274NADUniRule annotation2

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processHistidine metabolism
LigandNAD

Enzyme and pathway databases

UniPathwayiUPA00379; UER00550.

Names & Taxonomyi

Protein namesi
Recommended name:
Urocanate hydrataseUniRule annotation (EC:4.2.1.49UniRule annotation)
Short name:
UrocanaseUniRule annotation
Alternative name(s):
Imidazolonepropionate hydrolaseUniRule annotation
Gene namesi
Name:hutUUniRule annotation
Ordered Locus Names:KPK_3778
OrganismiKlebsiella pneumoniae (strain 342)
Taxonomic identifieri507522 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeKlebsiella
Proteomesi
  • UP000001734 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10001295651 – 562Urocanate hydrataseAdd BLAST562

Structurei

3D structure databases

SMRiB5XZ80.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the urocanase family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000237606.
KOiK01712.
OMAiVSFHHGG.

Family and domain databases

HAMAPiMF_00577. HutU. 1 hit.
InterProiView protein in InterPro
IPR023637. Urocanase.
IPR035401. Urocanase_C.
IPR023636. Urocanase_CS.
IPR035400. Urocanase_N.
IPR035085. Urocanase_Rossmann-like.
IPR036190. Urocanase_sf.
PANTHERiPTHR12216. PTHR12216. 1 hit.
PfamiView protein in Pfam
PF01175. Urocanase. 1 hit.
PF17392. Urocanase_C. 1 hit.
PF17391. Urocanase_N. 1 hit.
PIRSFiPIRSF001423. Urocanate_hydrat. 1 hit.
SUPFAMiSSF111326. SSF111326. 1 hit.
TIGRFAMsiTIGR01228. hutU. 1 hit.
PROSITEiView protein in PROSITE
PS01233. UROCANASE. 1 hit.

Sequencei

Sequence statusi: Complete.

B5XZ80-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSQSKYRQLD VRAPRGTTLT ARSWLTEAPL RMLMNNLDPD VAENPHELVV
60 70 80 90 100
YGGIGRAARN WECYDAIVKA LKNLESDETL LVQSGKPVGV FKTHENSPRV
110 120 130 140 150
LIANSNLVPH WATWEHFNEL DAKGLAMYGQ MTAGSWIYIG SQGIVQGTYE
160 170 180 190 200
TFVEAGRQHY QGSLKGRWVL TAGLGGMGGA QPLAATLAGA CSLNIECQQS
210 220 230 240 250
RIDFRLRTRY VDEQATSLDD ALARIKKYTA EGRAISIALC GNAADIVPEL
260 270 280 290 300
VKRGVRPDMV TDQTSAHDPL HGYLPKGWSW EEYQQKAESD PQGTILAAKR
310 320 330 340 350
SMADHVQAML AFNEMGVPTF DYGNNIRQMA QEMGVSNAFD FPGFVPAYIR
360 370 380 390 400
PLFCRGIGPF RWVALSGDPQ DIYKTDAKVK EIIKDDKHLH HWLDMARERI
410 420 430 440 450
SFQGLPARIC WVGLEWRQKL GLAFNEMVRS GEVSAPIVIG RDHLDSGSVA
460 470 480 490 500
SPNRETEAMR DGSDAVSDWP LLNALLNTAS GATWVSLHHG GGVGMGFSQH
510 520 530 540 550
SGMVIVCDGT DEAAARIARV LRNDPATGVM RHADAGYDIA IECAAEQGLN
560
LPMVAATQGN AK
Length:562
Mass (Da):61,562
Last modified:November 25, 2008 - v1
Checksum:i8F984F14F7AC1AC3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000964 Genomic DNA. Translation: ACI09126.1.
RefSeqiWP_012542404.1. NC_011283.1.

Genome annotation databases

EnsemblBacteriaiACI09126; ACI09126; KPK_3778.
KEGGikpe:KPK_3778.

Similar proteinsi

Entry informationi

Entry nameiHUTU_KLEP3
AccessioniPrimary (citable) accession number: B5XZ80
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: November 25, 2008
Last modified: November 22, 2017
This is version 58 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families