Reviewed, UniProtKB/Swiss-Prot B5XYC7 (ACEK_KLEP3)

Names and origin

Protein names

Recommended name:
    Isocitrate dehydrogenase kinase/phosphatase
      Short name=IDH kinase/phosphatase
      Short name=IDHK/P
    EC=2.7.11.5
    EC=3.1.3.-

Gene names

Name:	aceK
Ordered Locus Names:	KPK_5277

Organism

Klebsiella pneumoniae (strain 342) [Complete proteome] [HAMAP]

Taxonomic identifier

507522 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Klebsiella

Protein attributes

Sequence length	594 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Bifunctional enzyme which can phosphorylate or dephosphorylate isocitrate dehydrogenase (IDH) on a specific serine residue. This is a regulatory mechanism which enables bacteria to bypass the Krebs cycle via the glyoxylate shunt in response to the source of carbon. When bacteria are grown on glucose, IDH is fully active and unphosphorylated, but when grown on acetate or ethanol, the activity of IDH declines drastically concomitant with its phosphorylation By similarity.
Catalytic activity	ATP + [isocitrate dehydrogenase (NADP+)] = ADP + [isocitrate dehydrogenase (NADP+)] phosphate. HAMAP MF_00747
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the aceK family.

Ontologies

Keywords
Biological process	Glyoxylate bypass Tricarboxylic acid cycle
Cellular component	Cytoplasm
Ligand	ATP-binding NADP Nucleotide-binding
Molecular function	Hydrolase Kinase Protein phosphatase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glucose metabolic process Inferred from electronic annotation. Source: InterPro glyoxylate cycle Inferred from electronic annotation. Source: HAMAP tricarboxylic acid cycle Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP [isocitrate dehydrogenase (NADP+)] kinase activity Inferred from electronic annotation. Source: HAMAP phosphoprotein phosphatase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 594	594	Isocitrate dehydrogenase kinase/phosphatase HAMAP MF_00747		PRO_1000133272
Regions
	Nucleotide binding	315 – 321	7	ATP By similarity
Sites
	Active site	371	1	By similarity
	Binding site	336	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	B5XYC7-1 [UniParc]. Last modified November 25, 2008. Version 1. Checksum: 2FAAB720D4765DAC	FASTA	594	68,841
	10 20 30 40 50 60 MTRGLELLIA QTILQGFDAQ YGRFLEVTSG AQQRFEQADW HAVQQAMKQR IHLYDHHVGL 70 80 90 100 110 120 VVEQLRCITE GKSTDVDFLL RVKQQYTQLL PDYPRFEIAE SFFNSVYCRL FDHRSLTPER 130 140 150 160 170 180 LFIFSSQPER PFRTLPRPLA KDFFPERGWS HLLGKVLSDL PLRLAWQNKA RDIGYIIASL 190 200 210 220 230 240 QEALGEELLA TCHLQVANEL FYRNKAAWLV GKLVMPMATL PFLLPIHRSD EGELFVDTCL 250 260 270 280 290 300 TTHAEASIVF GFARSYFMVY APLPGALVEW LREILPGKTT AELYMAIGCQ KHAKTESYRE 310 320 330 340 350 360 YLHYITRCDE QFIEAPGIRG MVMLVFTLPG FDRVFKVIKD RFAPQKEVTA AHVRACYQLV 370 380 390 400 410 420 KEHDRVGRMA DTQEFENFVL DKRQIAPGLL ALLQAEAGNK LTDLGDRIVI SHLYIERRMV 430 440 450 460 470 480 PLNLWLEQVN GQALRDAVEE YGNAIRQLAA ANIFPGDMLF KNFGVTRHGR VVFYDYDEIC 490 500 510 520 530 540 YMTEVNFRQI PPPRYPEDEL ASEPWYSVSP GDVFPEEFRH WLCADPRIGP LFEEMHADLL 550 560 570 580 590 RADYWRELQT RIKNGHVEDV YAYRRKQRFS VRYGADSRPD KVFTPPSGRV RRSA

References

[1]

"Complete genome sequence of the N2-fixing broad host range endophyte Klebsiella pneumoniae 342 and virulence predictions verified in mice." Fouts D.E., Tyler H.L., DeBoy R.T., Daugherty S., Ren Q., Badger J.H., Durkin A.S., Huot H., Shrivastava S., Kothari S., Dodson R.J., Mohamoud Y., Khouri H., Roesch L.F.W., Krogfelt K.A., Struve C., Triplett E.W., Methe B.A. PLoS Genet. 4:E1000141-E1000141(2008) [PubMed: 18654632] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000964 Genomic DNA. Translation: ACI07745.1.
RefSeq	YP_002241048.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	6935924.
GenomeReviews	Gene locus KPK_5277 in contig CP000964_GR.
KEGG	kpe:KPK_5277.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	FYRDFLH.
Family and domain databases
HAMAP	MF_00747. [Tree]
InterPro	IPR010452. Isocitrate_DH_AceK. [Graphical view]
Pfam	PF06315. AceK. 1 hit. [Graphical view]
PIRSF	PIRSF000719. AceK. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ACEK_KLEP3

Accession

Primary (citable) accession number: B5XYC7

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	November 25, 2008
Last modified:	November 3, 2009
This is version 11 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)