B5XXZ1 (PLSB_KLEP3) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glycerol-3-phosphate acyltransferase
Short name=GPAT
EC=2.3.1.15

Gene names

Name:	plsB
Ordered Locus Names:	KPK_5247

Organism

Klebsiella pneumoniae (strain 342) [Complete proteome] [HAMAP]

Taxonomic identifier

507522 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Klebsiella

Protein attributes

Sequence length	807 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Acyl-CoA + sn-glycerol 3-phosphate = CoA + 1-acyl-sn-glycerol 3-phosphate. HAMAP MF_00393
Pathway	Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-diacylglycerol from sn-glycerol 3-phosphate: step 1/3. HAMAP MF_00393
Subcellular location	Cell inner membrane; Peripheral membrane protein; Cytoplasmic side By similarity HAMAP MF_00393.
Domain	The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate By similarity. HAMAP MF_00393
Sequence similarities	Belongs to the GPAT/DAPAT family.

Ontologies

Keywords
Biological process	Phospholipid biosynthesis
Cellular component	Cell inner membrane Cell membrane Membrane
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phospholipid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	glycerol-3-phosphate O-acyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 807	807	Glycerol-3-phosphate acyltransferase HAMAP MF_00393		PRO_1000123084
Regions
	Motif	305 – 310	6	HXXXXD motif HAMAP MF_00393

Sequences

	Sequence		Length	Mass (Da)
	B5XXZ1 [UniParc]. Last modified November 25, 2008. Version 1. Checksum: B716C0A7FC3CB826	FASTA	807	91,447
	10 20 30 40 50 60 MSGWQRIYYK LLNLPLRVLV KSKSIPAEPA QELGLDTSRP VMYVLPYNSK ADLLTLRAQC 70 80 90 100 110 120 LAHDLPDPLE PLEIDGALLP RYVFIHGGPR VFTYYTPKEE SIKLFHDYLD LHRNHPDLDV 130 140 150 160 170 180 QMVPVSVMFG RSPGREKGEV NPPLRMLNGI QKFFAVSWLG RDSFVRFSPS VSLRRMADEH 190 200 210 220 230 240 GTDKIIAQKL ARVARMHFAR QRLAAVGPRL PARQDLFNKL LASKAIARAV EDEARSKKIS 250 260 270 280 290 300 HEKAQQNAIA LMEEIAANFS YEMIRLTDRI LGFTWNRLYQ GINVHNAERV RQLAHDGHEI 310 320 330 340 350 360 VYVPCHRSHM DYLLLSYVLY HQGLVPPHIA AGINLNFWPA GPIFRRLGAF FIRRTFKGNK 370 380 390 400 410 420 LYSTVFREYL GELFSRGYSV EYFVEGGRSR TGRLLDPKTG TLSMTIQAML RGGTRPITLV 430 440 450 460 470 480 PIYIGYEHVM EVGTYAKELR GATKEKESLP QMVRGLSKLR NLGQGYVNFG EPLPLMTYLN 490 500 510 520 530 540 HHVPEWREAI DPIEAIRPSW LTPTVNNIAA DLMVRINNAG AANAMNLCCT ALLASRQRSL 550 560 570 580 590 600 TREQLTQQLE CYLALLRNVP YSPDATTPSA SASELIDHAL QMNKFEVEKD TIGDIIILPR 610 620 630 640 650 660 EQAVLMTYYR NNIAHMLVIP SLLAALVTQH RQLSRTEVLR HVETLYPFLK AELFLRWEKA 670 680 690 700 710 720 ELAGVVDALI AEMLRQELIV VDGEVMSLNP SHSRSLQLLA AGARETLQRY AITFWLLSAN 730 740 750 760 770 780 PSINRSSLEK ESRTVAQRLS VLHGINAPEF FDKAVFSTLV LTLRDEGYIS DTGDAEPEET 790 800 LKVYRMLADL ITSDVRLTIE SVTQDDA

References

[1]

"Complete genome sequence of the N2-fixing broad host range endophyte Klebsiella pneumoniae 342 and virulence predictions verified in mice." Fouts D.E., Tyler H.L., DeBoy R.T., Daugherty S., Ren Q., Badger J.H., Durkin A.S., Huot H., Shrivastava S., Kothari S., Dodson R.J., Mohamoud Y., Khouri H., Roesch L.F.W., Krogfelt K.A., Struve C., Triplett E.W., Methe B.A. PLoS Genet. 4:E1000141-E1000141(2008) [PubMed: 18654632] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 342.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000964 Genomic DNA. Translation: ACI07938.1.
RefSeq	YP_002241018.1. NC_011283.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	B5XXZ1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6935452.
GenomeReviews	Gene locus KPK_5247 in contig CP000964_GR.
KEGG	kpe:KPK_5247.
PATRIC	20443592. VBIKlePne121904_5124.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG296590.
OMA	WNKLYQG.
ProtClustDB	PRK04974.
Family and domain databases
HAMAP	MF_00393. Glyc3P_acyltrans. [Tree]
InterPro	IPR002123. Acyltransferase. IPR022284. G3P_O-AcylTrfase. [Graphical view]
KO	K00631.
Pfam	PF01553. Acyltransferase. 1 hit. [Graphical view]
PIRSF	PIRSF000437. GPAT_DHAPAT. 1 hit.
SMART	SM00563. PlsC. 1 hit. [Graphical view]
TIGRFAMs	TIGR03703. PlsB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PLSB_KLEP3

Accession

Primary (citable) accession number: B5XXZ1

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	November 25, 2008
Last modified:	January 25, 2012
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program