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Protein

Glycerol-3-phosphate acyltransferase

Gene

plsB

Organism
Klebsiella pneumoniae (strain 342)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

Acyl-CoA + sn-glycerol 3-phosphate = CoA + 1-acyl-sn-glycerol 3-phosphate.UniRule annotation

Pathwayi: CDP-diacylglycerol biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes CDP-diacylglycerol from sn-glycerol 3-phosphate.UniRule annotation
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Glycerol-3-phosphate acyltransferase (plsB)
  2. no protein annotated in this organism
  3. Phosphatidate cytidylyltransferase (cdsA)
This subpathway is part of the pathway CDP-diacylglycerol biosynthesis, which is itself part of Phospholipid metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes CDP-diacylglycerol from sn-glycerol 3-phosphate, the pathway CDP-diacylglycerol biosynthesis and in Phospholipid metabolism.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism

Enzyme and pathway databases

BioCyciKPNE507522:GI0B-5248-MONOMER.
UniPathwayiUPA00557; UER00612.

Names & Taxonomyi

Protein namesi
Recommended name:
Glycerol-3-phosphate acyltransferaseUniRule annotation (EC:2.3.1.15UniRule annotation)
Short name:
GPATUniRule annotation
Gene namesi
Name:plsBUniRule annotation
Ordered Locus Names:KPK_5247
OrganismiKlebsiella pneumoniae (strain 342)
Taxonomic identifieri507522 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeKlebsiella
Proteomesi
  • UP000001734 Componenti: Chromosome

Subcellular locationi

  • Cell inner membrane UniRule annotation; Peripheral membrane protein UniRule annotation; Cytoplasmic side UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 807807Glycerol-3-phosphate acyltransferasePRO_1000123084Add
BLAST

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi305 – 3106HXXXXD motif

Domaini

The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate.UniRule annotation

Sequence similaritiesi

Belongs to the GPAT/DAPAT family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000218231.
KOiK00631.
OMAiEVIYVPC.
OrthoDBiEOG6B625B.

Family and domain databases

HAMAPiMF_00393. Glyc3P_acyltrans.
InterProiIPR022284. GPAT/DHAPAT.
IPR028354. GPAT_PlsB.
IPR002123. Plipid/glycerol_acylTrfase.
[Graphical view]
PANTHERiPTHR12563. PTHR12563. 2 hits.
PfamiPF01553. Acyltransferase. 1 hit.
[Graphical view]
PIRSFiPIRSF500064. GPAT. 1 hit.
PIRSF000437. GPAT_DHAPAT. 1 hit.
SMARTiSM00563. PlsC. 1 hit.
[Graphical view]
TIGRFAMsiTIGR03703. plsB. 1 hit.

Sequencei

Sequence statusi: Complete.

B5XXZ1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSGWQRIYYK LLNLPLRVLV KSKSIPAEPA QELGLDTSRP VMYVLPYNSK
60 70 80 90 100
ADLLTLRAQC LAHDLPDPLE PLEIDGALLP RYVFIHGGPR VFTYYTPKEE
110 120 130 140 150
SIKLFHDYLD LHRNHPDLDV QMVPVSVMFG RSPGREKGEV NPPLRMLNGI
160 170 180 190 200
QKFFAVSWLG RDSFVRFSPS VSLRRMADEH GTDKIIAQKL ARVARMHFAR
210 220 230 240 250
QRLAAVGPRL PARQDLFNKL LASKAIARAV EDEARSKKIS HEKAQQNAIA
260 270 280 290 300
LMEEIAANFS YEMIRLTDRI LGFTWNRLYQ GINVHNAERV RQLAHDGHEI
310 320 330 340 350
VYVPCHRSHM DYLLLSYVLY HQGLVPPHIA AGINLNFWPA GPIFRRLGAF
360 370 380 390 400
FIRRTFKGNK LYSTVFREYL GELFSRGYSV EYFVEGGRSR TGRLLDPKTG
410 420 430 440 450
TLSMTIQAML RGGTRPITLV PIYIGYEHVM EVGTYAKELR GATKEKESLP
460 470 480 490 500
QMVRGLSKLR NLGQGYVNFG EPLPLMTYLN HHVPEWREAI DPIEAIRPSW
510 520 530 540 550
LTPTVNNIAA DLMVRINNAG AANAMNLCCT ALLASRQRSL TREQLTQQLE
560 570 580 590 600
CYLALLRNVP YSPDATTPSA SASELIDHAL QMNKFEVEKD TIGDIIILPR
610 620 630 640 650
EQAVLMTYYR NNIAHMLVIP SLLAALVTQH RQLSRTEVLR HVETLYPFLK
660 670 680 690 700
AELFLRWEKA ELAGVVDALI AEMLRQELIV VDGEVMSLNP SHSRSLQLLA
710 720 730 740 750
AGARETLQRY AITFWLLSAN PSINRSSLEK ESRTVAQRLS VLHGINAPEF
760 770 780 790 800
FDKAVFSTLV LTLRDEGYIS DTGDAEPEET LKVYRMLADL ITSDVRLTIE

SVTQDDA
Length:807
Mass (Da):91,447
Last modified:November 25, 2008 - v1
Checksum:iB716C0A7FC3CB826
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000964 Genomic DNA. Translation: ACI07938.1.
RefSeqiWP_008807307.1. NC_011283.1.

Genome annotation databases

EnsemblBacteriaiACI07938; ACI07938; KPK_5247.
KEGGikpe:KPK_5247.
PATRICi20443592. VBIKlePne121904_5124.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000964 Genomic DNA. Translation: ACI07938.1.
RefSeqiWP_008807307.1. NC_011283.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACI07938; ACI07938; KPK_5247.
KEGGikpe:KPK_5247.
PATRICi20443592. VBIKlePne121904_5124.

Phylogenomic databases

HOGENOMiHOG000218231.
KOiK00631.
OMAiEVIYVPC.
OrthoDBiEOG6B625B.

Enzyme and pathway databases

UniPathwayiUPA00557; UER00612.
BioCyciKPNE507522:GI0B-5248-MONOMER.

Family and domain databases

HAMAPiMF_00393. Glyc3P_acyltrans.
InterProiIPR022284. GPAT/DHAPAT.
IPR028354. GPAT_PlsB.
IPR002123. Plipid/glycerol_acylTrfase.
[Graphical view]
PANTHERiPTHR12563. PTHR12563. 2 hits.
PfamiPF01553. Acyltransferase. 1 hit.
[Graphical view]
PIRSFiPIRSF500064. GPAT. 1 hit.
PIRSF000437. GPAT_DHAPAT. 1 hit.
SMARTiSM00563. PlsC. 1 hit.
[Graphical view]
TIGRFAMsiTIGR03703. plsB. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Complete genome sequence of the N2-fixing broad host range endophyte Klebsiella pneumoniae 342 and virulence predictions verified in mice."
    Fouts D.E., Tyler H.L., DeBoy R.T., Daugherty S., Ren Q., Badger J.H., Durkin A.S., Huot H., Shrivastava S., Kothari S., Dodson R.J., Mohamoud Y., Khouri H., Roesch L.F.W., Krogfelt K.A., Struve C., Triplett E.W., Methe B.A.
    PLoS Genet. 4:E1000141-E1000141(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 342.

Entry informationi

Entry nameiPLSB_KLEP3
AccessioniPrimary (citable) accession number: B5XXZ1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: November 25, 2008
Last modified: November 11, 2015
This is version 48 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.