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B5XXX7 (APHA_KLEP3) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 33. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Class B acid phosphatase

Short name=CBAP
EC=3.1.3.2
Gene names
Name:aphA
Ordered Locus Names:KPK_5233
OrganismKlebsiella pneumoniae (strain 342) [Complete proteome] [HAMAP]
Taxonomic identifier507522 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeKlebsiella

Protein attributes

Sequence length237 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Dephosphorylates several organic phosphate monoesters. Also has a phosphotransferase activity catalyzing the transfer of low-energy phosphate groups from organic phosphate monoesters to free hydroxyl groups of various organic compounds By similarity.

Catalytic activity

A phosphate monoester + H2O = an alcohol + phosphate.

Cofactor

Binds 1 magnesium ion per subunit By similarity.

Subunit structure

Homotetramer By similarity.

Subcellular location

Periplasm By similarity.

Sequence similarities

Belongs to the class B bacterial acid phosphatase family.

Ontologies

Keywords
   Cellular componentPeriplasm
   DomainSignal
   LigandMagnesium
Metal-binding
   Molecular functionHydrolase
   Technical termComplete proteome
Gene Ontology (GO)
   Cellular_componentouter membrane-bounded periplasmic space

Inferred from electronic annotation. Source: InterPro

   Molecular_functionacid phosphatase activity

Inferred from electronic annotation. Source: UniProtKB-EC

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2525 Potential
Chain26 – 237212Class B acid phosphatase
PRO_0000415227

Regions

Region137 – 1382Substrate binding By similarity

Sites

Active site691Nucleophile By similarity
Active site711Proton donor By similarity
Metal binding691Magnesium By similarity
Metal binding711Magnesium; via carbonyl oxygen By similarity
Metal binding1921Magnesium By similarity
Binding site1771Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
B5XXX7 [UniParc].

Last modified November 25, 2008. Version 1.
Checksum: 8E0A31C5A8EB5721

FASTA23726,137
        10         20         30         40         50         60 
MRKLTLAFAA ASLLFTLNSA VVARASTPQP LWVGTNVAQL AEQAPIHWVS VAQIENSLLG 

        70         80         90        100        110        120 
RPPMAVGFDI DDTVLFSSPG FWRGQKTFSP GSEDYLKNPQ FWEKMNNGWD EFSMPKEVAR 

       130        140        150        160        170        180 
QLIAMHVKRG DSIWFVTGRS QTKTETVSKT LQDDFLIPAA NMNPVIFAGD KPGQNTKTQW 

       190        200        210        220        230 
LQAKQIKVFY GDSDNDITAA REAGARGIRV LRAANSSYKP LPMAGALGEE VIVNSEY 

« Hide

References

[1]"Complete genome sequence of the N2-fixing broad host range endophyte Klebsiella pneumoniae 342 and virulence predictions verified in mice."
Fouts D.E., Tyler H.L., DeBoy R.T., Daugherty S., Ren Q., Badger J.H., Durkin A.S., Huot H., Shrivastava S., Kothari S., Dodson R.J., Mohamoud Y., Khouri H., Roesch L.F.W., Krogfelt K.A., Struve C., Triplett E.W., Methe B.A.
PLoS Genet. 4:E1000141-E1000141(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 342.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000964 Genomic DNA. Translation: ACI07686.1.
RefSeqYP_002241004.1. NC_011283.1.

3D structure databases

ProteinModelPortalB5XXX7.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING507522.KPK_5233.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACI07686; ACI07686; KPK_5233.
GeneID6935673.
KEGGkpe:KPK_5233.
PATRIC20443566. VBIKlePne121904_5111.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG3700.
HOGENOMHOG000270623.
KOK03788.
OMAPEFWEKM.
OrthoDBEOG6HTNVZ.

Enzyme and pathway databases

BioCycKPNE507522:GI0B-5234-MONOMER.

Family and domain databases

Gene3D3.40.50.1000. 1 hit.
InterProIPR005519. Acid_phosphat_B.
IPR023214. HAD-like_dom.
IPR010025. HAD-SF_ppase_IIIB_AphA.
[Graphical view]
PfamPF03767. Acid_phosphat_B. 1 hit.
[Graphical view]
PIRSFPIRSF017818. Acid_Ptase_B. 1 hit.
SUPFAMSSF56784. SSF56784. 1 hit.
TIGRFAMsTIGR01672. AphA. 1 hit.
ProtoNetSearch...

Entry information

Entry nameAPHA_KLEP3
AccessionPrimary (citable) accession number: B5XXX7
Entry history
Integrated into UniProtKB/Swiss-Prot: January 25, 2012
Last sequence update: November 25, 2008
Last modified: May 14, 2014
This is version 33 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families