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B5XXX7

- APHA_KLEP3

UniProt

B5XXX7 - APHA_KLEP3

Protein

Class B acid phosphatase

Gene

aphA

Organism
Klebsiella pneumoniae (strain 342)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 34 (01 Oct 2014)
      Sequence version 1 (25 Nov 2008)
      Previous versions | rss
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    Functioni

    Dephosphorylates several organic phosphate monoesters. Also has a phosphotransferase activity catalyzing the transfer of low-energy phosphate groups from organic phosphate monoesters to free hydroxyl groups of various organic compounds By similarity.By similarity

    Catalytic activityi

    A phosphate monoester + H2O = an alcohol + phosphate.By similarity

    Cofactori

    Binds 1 magnesium ion per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei69 – 691NucleophileBy similarity
    Metal bindingi69 – 691MagnesiumBy similarity
    Active sitei71 – 711Proton donorBy similarity
    Metal bindingi71 – 711Magnesium; via carbonyl oxygenBy similarity
    Binding sitei177 – 1771SubstrateBy similarity
    Metal bindingi192 – 1921MagnesiumBy similarity

    GO - Molecular functioni

    1. acid phosphatase activity Source: UniProtKB-EC
    2. metal ion binding Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Ligandi

    Magnesium, Metal-binding

    Enzyme and pathway databases

    BioCyciKPNE507522:GI0B-5234-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Class B acid phosphataseImportedBy similarity (EC:3.1.3.2ImportedBy similarity)
    Short name:
    CBAPBy similarity
    Gene namesi
    Name:aphAImported
    Ordered Locus Names:KPK_5233
    OrganismiKlebsiella pneumoniae (strain 342)
    Taxonomic identifieri507522 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeKlebsiella
    ProteomesiUP000001734: Chromosome

    Subcellular locationi

    Periplasm By similarity

    GO - Cellular componenti

    1. outer membrane-bounded periplasmic space Source: InterPro

    Keywords - Cellular componenti

    Periplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2525Sequence AnalysisAdd
    BLAST
    Chaini26 – 237212Class B acid phosphataseSequence AnalysisPRO_0000415227Add
    BLAST

    Interactioni

    Subunit structurei

    Homotetramer.By similarity

    Protein-protein interaction databases

    STRINGi507522.KPK_5233.

    Structurei

    3D structure databases

    ProteinModelPortaliB5XXX7.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni137 – 1382Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the class B bacterial acid phosphatase family.By similarity

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG3700.
    HOGENOMiHOG000270623.
    KOiK03788.
    OMAiPEFWEKM.
    OrthoDBiEOG6HTNVZ.

    Family and domain databases

    Gene3Di3.40.50.1000. 1 hit.
    InterProiIPR005519. Acid_phosphat_B.
    IPR023214. HAD-like_dom.
    IPR010025. HAD-SF_ppase_IIIB_AphA.
    [Graphical view]
    PfamiPF03767. Acid_phosphat_B. 1 hit.
    [Graphical view]
    PIRSFiPIRSF017818. Acid_Ptase_B. 1 hit.
    SUPFAMiSSF56784. SSF56784. 1 hit.
    TIGRFAMsiTIGR01672. AphA. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    B5XXX7-1 [UniParc]FASTAAdd to Basket

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    MRKLTLAFAA ASLLFTLNSA VVARASTPQP LWVGTNVAQL AEQAPIHWVS    50
    VAQIENSLLG RPPMAVGFDI DDTVLFSSPG FWRGQKTFSP GSEDYLKNPQ 100
    FWEKMNNGWD EFSMPKEVAR QLIAMHVKRG DSIWFVTGRS QTKTETVSKT 150
    LQDDFLIPAA NMNPVIFAGD KPGQNTKTQW LQAKQIKVFY GDSDNDITAA 200
    REAGARGIRV LRAANSSYKP LPMAGALGEE VIVNSEY 237
    Length:237
    Mass (Da):26,137
    Last modified:November 25, 2008 - v1
    Checksum:i8E0A31C5A8EB5721
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000964 Genomic DNA. Translation: ACI07686.1.
    RefSeqiYP_002241004.1. NC_011283.1.

    Genome annotation databases

    EnsemblBacteriaiACI07686; ACI07686; KPK_5233.
    GeneIDi6935673.
    KEGGikpe:KPK_5233.
    PATRICi20443566. VBIKlePne121904_5111.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000964 Genomic DNA. Translation: ACI07686.1 .
    RefSeqi YP_002241004.1. NC_011283.1.

    3D structure databases

    ProteinModelPortali B5XXX7.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 507522.KPK_5233.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ACI07686 ; ACI07686 ; KPK_5233 .
    GeneIDi 6935673.
    KEGGi kpe:KPK_5233.
    PATRICi 20443566. VBIKlePne121904_5111.

    Phylogenomic databases

    eggNOGi COG3700.
    HOGENOMi HOG000270623.
    KOi K03788.
    OMAi PEFWEKM.
    OrthoDBi EOG6HTNVZ.

    Enzyme and pathway databases

    BioCyci KPNE507522:GI0B-5234-MONOMER.

    Family and domain databases

    Gene3Di 3.40.50.1000. 1 hit.
    InterProi IPR005519. Acid_phosphat_B.
    IPR023214. HAD-like_dom.
    IPR010025. HAD-SF_ppase_IIIB_AphA.
    [Graphical view ]
    Pfami PF03767. Acid_phosphat_B. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF017818. Acid_Ptase_B. 1 hit.
    SUPFAMi SSF56784. SSF56784. 1 hit.
    TIGRFAMsi TIGR01672. AphA. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Complete genome sequence of the N2-fixing broad host range endophyte Klebsiella pneumoniae 342 and virulence predictions verified in mice."
      Fouts D.E., Tyler H.L., DeBoy R.T., Daugherty S., Ren Q., Badger J.H., Durkin A.S., Huot H., Shrivastava S., Kothari S., Dodson R.J., Mohamoud Y., Khouri H., Roesch L.F.W., Krogfelt K.A., Struve C., Triplett E.W., Methe B.A.
      PLoS Genet. 4:E1000141-E1000141(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 3421 Publication.

    Entry informationi

    Entry nameiAPHA_KLEP3
    AccessioniPrimary (citable) accession number: B5XXX7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 25, 2012
    Last sequence update: November 25, 2008
    Last modified: October 1, 2014
    This is version 34 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3