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B5XXX7

- APHA_KLEP3

UniProt

B5XXX7 - APHA_KLEP3

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Protein
Class B acid phosphatase
Gene
aphA, KPK_5233
Organism
Klebsiella pneumoniae (strain 342)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Dephosphorylates several organic phosphate monoesters. Also has a phosphotransferase activity catalyzing the transfer of low-energy phosphate groups from organic phosphate monoesters to free hydroxyl groups of various organic compounds By similarity.

Catalytic activityi

A phosphate monoester + H2O = an alcohol + phosphate.

Cofactori

Binds 1 magnesium ion per subunit By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei69 – 691Nucleophile By similarity
Metal bindingi69 – 691Magnesium By similarity
Active sitei71 – 711Proton donor By similarity
Metal bindingi71 – 711Magnesium; via carbonyl oxygen By similarity
Binding sitei177 – 1771Substrate By similarity
Metal bindingi192 – 1921Magnesium By similarity

GO - Molecular functioni

  1. acid phosphatase activity Source: UniProtKB-EC
  2. metal ion binding Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciKPNE507522:GI0B-5234-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Class B acid phosphatase (EC:3.1.3.2)
Short name:
CBAP
Gene namesi
Name:aphA
Ordered Locus Names:KPK_5233
OrganismiKlebsiella pneumoniae (strain 342)
Taxonomic identifieri507522 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeKlebsiella
ProteomesiUP000001734: Chromosome

Subcellular locationi

Periplasm By similarity

GO - Cellular componenti

  1. outer membrane-bounded periplasmic space Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2525 Reviewed prediction
Add
BLAST
Chaini26 – 237212Class B acid phosphatase
PRO_0000415227Add
BLAST

Interactioni

Subunit structurei

Homotetramer By similarity.

Protein-protein interaction databases

STRINGi507522.KPK_5233.

Structurei

3D structure databases

ProteinModelPortaliB5XXX7.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni137 – 1382Substrate binding By similarity

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG3700.
HOGENOMiHOG000270623.
KOiK03788.
OMAiPEFWEKM.
OrthoDBiEOG6HTNVZ.

Family and domain databases

Gene3Di3.40.50.1000. 1 hit.
InterProiIPR005519. Acid_phosphat_B.
IPR023214. HAD-like_dom.
IPR010025. HAD-SF_ppase_IIIB_AphA.
[Graphical view]
PfamiPF03767. Acid_phosphat_B. 1 hit.
[Graphical view]
PIRSFiPIRSF017818. Acid_Ptase_B. 1 hit.
SUPFAMiSSF56784. SSF56784. 1 hit.
TIGRFAMsiTIGR01672. AphA. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

B5XXX7-1 [UniParc]FASTAAdd to Basket

« Hide

MRKLTLAFAA ASLLFTLNSA VVARASTPQP LWVGTNVAQL AEQAPIHWVS    50
VAQIENSLLG RPPMAVGFDI DDTVLFSSPG FWRGQKTFSP GSEDYLKNPQ 100
FWEKMNNGWD EFSMPKEVAR QLIAMHVKRG DSIWFVTGRS QTKTETVSKT 150
LQDDFLIPAA NMNPVIFAGD KPGQNTKTQW LQAKQIKVFY GDSDNDITAA 200
REAGARGIRV LRAANSSYKP LPMAGALGEE VIVNSEY 237
Length:237
Mass (Da):26,137
Last modified:November 25, 2008 - v1
Checksum:i8E0A31C5A8EB5721
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000964 Genomic DNA. Translation: ACI07686.1.
RefSeqiYP_002241004.1. NC_011283.1.

Genome annotation databases

EnsemblBacteriaiACI07686; ACI07686; KPK_5233.
GeneIDi6935673.
KEGGikpe:KPK_5233.
PATRICi20443566. VBIKlePne121904_5111.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000964 Genomic DNA. Translation: ACI07686.1 .
RefSeqi YP_002241004.1. NC_011283.1.

3D structure databases

ProteinModelPortali B5XXX7.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 507522.KPK_5233.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACI07686 ; ACI07686 ; KPK_5233 .
GeneIDi 6935673.
KEGGi kpe:KPK_5233.
PATRICi 20443566. VBIKlePne121904_5111.

Phylogenomic databases

eggNOGi COG3700.
HOGENOMi HOG000270623.
KOi K03788.
OMAi PEFWEKM.
OrthoDBi EOG6HTNVZ.

Enzyme and pathway databases

BioCyci KPNE507522:GI0B-5234-MONOMER.

Family and domain databases

Gene3Di 3.40.50.1000. 1 hit.
InterProi IPR005519. Acid_phosphat_B.
IPR023214. HAD-like_dom.
IPR010025. HAD-SF_ppase_IIIB_AphA.
[Graphical view ]
Pfami PF03767. Acid_phosphat_B. 1 hit.
[Graphical view ]
PIRSFi PIRSF017818. Acid_Ptase_B. 1 hit.
SUPFAMi SSF56784. SSF56784. 1 hit.
TIGRFAMsi TIGR01672. AphA. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Complete genome sequence of the N2-fixing broad host range endophyte Klebsiella pneumoniae 342 and virulence predictions verified in mice."
    Fouts D.E., Tyler H.L., DeBoy R.T., Daugherty S., Ren Q., Badger J.H., Durkin A.S., Huot H., Shrivastava S., Kothari S., Dodson R.J., Mohamoud Y., Khouri H., Roesch L.F.W., Krogfelt K.A., Struve C., Triplett E.W., Methe B.A.
    PLoS Genet. 4:E1000141-E1000141(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 342.

Entry informationi

Entry nameiAPHA_KLEP3
AccessioniPrimary (citable) accession number: B5XXX7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 25, 2012
Last sequence update: November 25, 2008
Last modified: May 14, 2014
This is version 33 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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