Reviewed, UniProtKB/Swiss-Prot B5XXG4 (NAGZ_KLEP3)

Names and origin

Protein names

Recommended name:
    Beta-hexosaminidase
    EC=3.2.1.52
Alternative name(s):
    N-acetyl-beta-glucosaminidase
    Beta-N-acetylhexosaminidase

Gene names

Name:	nagZ
Ordered Locus Names:	KPK_3452

Organism

Klebsiella pneumoniae (strain 342) [Complete proteome] [HAMAP]

Taxonomic identifier

507522 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Klebsiella

Protein attributes

Sequence length	340 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Cleaves GlcNAc linked beta-1,4 to MurNAc tripeptides By similarity.
Catalytic activity	Hydrolysis of terminal non-reducing N-acetyl-D-hexosamine residues in N-acetyl-beta-D-hexosaminides. HAMAP MF_00364
Pathway	Cell wall biogenesis; peptidoglycan recycling. HAMAP MF_00364
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the glycosyl hydrolase 3 family. NagZ subfamily.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Molecular function	Glycosidase Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan turnover Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	beta-N-acetylhexosaminidase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 340	340	Beta-hexosaminidase HAMAP MF_00364		PRO_1000121063
Sites
	Active site	248	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	B5XXG4-1 [UniParc]. Last modified November 25, 2008. Version 1. Checksum: C948838DF02E97B8	FASTA	340	37,383
	10 20 30 40 50 60 MGPVMLDVEG YELDAEEREI LAHPLVGGLI LFTRNYHDPA QLRELVRQIR DASRNHLVVA 70 80 90 100 110 120 VDQEGGRVQR FREGFTRLPA AQSFAALLGM EEGGKLAAEA GWLMASEMIA MDIDISFAPV 130 140 150 160 170 180 LDVGHISAAI GERSYHEDPQ KALTMARHFI DGMHAAGMKT TGKHFPGHGA VTADSHKETP 190 200 210 220 230 240 FDPRPASVIR DHDMAVFKSL IAEQRLDAIM PAHVIYPELD PRPASGSAYW LKTVLRGELG 250 260 270 280 290 300 FDGVIFSDDL SMEGAAIMGS YAERGQASLD AGCDMILVCN NRKGAVSVLD NLSPIKAERV 310 320 330 340 TKLYHKGSFS RQELRDSARW KTVSAQLEQL HARWQEAKSA

References

[1]

"Complete genome sequence of the N2-fixing broad host range endophyte Klebsiella pneumoniae 342 and virulence predictions verified in mice." Fouts D.E., Tyler H.L., DeBoy R.T., Daugherty S., Ren Q., Badger J.H., Durkin A.S., Huot H., Shrivastava S., Kothari S., Dodson R.J., Mohamoud Y., Khouri H., Roesch L.F.W., Krogfelt K.A., Struve C., Triplett E.W., Methe B.A. PLoS Genet. 4:E1000141-E1000141(2008) [PubMed: 18654632] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000964 Genomic DNA. Translation: ACI07330.1.
RefSeq	YP_002239275.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	6938483.
GenomeReviews	Gene locus KPK_3452 in contig CP000964_GR.
KEGG	kpe:KPK_3452.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	NIDSPRQ.
Family and domain databases
HAMAP	MF_00364. [Tree]
InterPro	IPR019800. Glyco_hydro_3_AS. IPR001764. Glyco_hydro_3_N. [Graphical view]
Gene3D	G3DSA:3.20.20.300. Glyco_hydro_3_N. 1 hit.
Pfam	PF00933. Glyco_hydro_3. 1 hit. [Graphical view]
PROSITE	PS00775. GLYCOSYL_HYDROL_F3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NAGZ_KLEP3

Accession

Primary (citable) accession number: B5XXG4

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	November 25, 2008
Last modified:	November 3, 2009
This is version 8 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)