B5XWQ7 (ADD_KLEP3) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Adenosine deaminase
EC=3.5.4.4

Alternative name(s):
Adenosine aminohydrolase

Gene names

Name:	add
Ordered Locus Names:	KPK_2390

Organism

Klebsiella pneumoniae (strain 342) [Complete proteome] [HAMAP]

Taxonomic identifier

507522 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Klebsiella

Protein attributes

Sequence length	333 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Adenosine + H2O = inosine + NH3. HAMAP MF_00540
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00540
Sequence similarities	Belongs to the adenosine and AMP deaminases family. Adenosine deaminase subfamily.

Ontologies

Keywords
Biological process	Nucleotide metabolism
Ligand	Metal-binding Zinc
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	purine ribonucleoside monophosphate biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	adenosine deaminase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 333	333	Adenosine deaminase HAMAP MF_00540		PRO_1000128849
Sites
	Active site	200	1	Proton donor By similarity
	Metal binding	12	1	Zinc; catalytic By similarity
	Metal binding	14	1	Zinc; catalytic By similarity
	Metal binding	197	1	Zinc; catalytic By similarity
	Metal binding	278	1	Zinc; catalytic By similarity
	Binding site	14	1	Substrate By similarity
	Binding site	16	1	Substrate By similarity
	Binding site	170	1	Substrate; via amide nitrogen and carbonyl oxygen By similarity
	Binding site	279	1	Substrate By similarity
	Site	221	1	Important for catalytic activity By similarity

Sequences

	Sequence		Length	Mass (Da)
	B5XWQ7 [UniParc]. Last modified November 25, 2008. Version 1. Checksum: 03D609962FD4E04A	FASTA	333	36,144
	10 20 30 40 50 60 MIDSSLPLTD IHRHLDGNIR AQTILDLGRE FNIALPASTL DTLRPHVQVT SLEPDLVSFL 70 80 90 100 110 120 AKLDWGVKVL ASLEACRRVA YENVEDAARN GLHYVELRFS PRYMAMTHQL PVDGVVEAVI 130 140 150 160 170 180 AGVREGSRDF QVDARLIGIL SRTFGEAACQ EELAALLAHR EGITALDLAG DELGFPGALF 190 200 210 220 230 240 LNHFNQARDA GWHITVHAGE AAGPESIWQA IRELGAERIG HGVKAVEDPA LMDYLAEHQI 250 260 270 280 290 300 GIESCLTSNV QTSTVASLAQ HPLKQFLEHG VLASLNTDDP AVQGVDIIHE YTVAAPAAGL 310 320 330 SREQIRQAQI NGLTQAFLSE QEKAALIQRV AKG

References

[1]

"Complete genome sequence of the N2-fixing broad host range endophyte Klebsiella pneumoniae 342 and virulence predictions verified in mice." Fouts D.E., Tyler H.L., DeBoy R.T., Daugherty S., Ren Q., Badger J.H., Durkin A.S., Huot H., Shrivastava S., Kothari S., Dodson R.J., Mohamoud Y., Khouri H., Roesch L.F.W., Krogfelt K.A., Struve C., Triplett E.W., Methe B.A. PLoS Genet. 4:E1000141-E1000141(2008) [PubMed: 18654632] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 342.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000964 Genomic DNA. Translation: ACI06910.1.
RefSeq	YP_002238221.1. NC_011283.1.
3D structure databases
ProteinModelPortal	B5XWQ7.
ModBase	Search...
Protein-protein interaction databases
STRING	B5XWQ7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6937294.
GenomeReviews	Gene locus KPK_2390 in contig CP000964_GR.
KEGG	kpe:KPK_2390.
PATRIC	20438211. VBIKlePne121904_2478.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG630382.
OMA	PYYMAMN.
ProtClustDB	CLSK2400760.
Family and domain databases
HAMAP	MF_00540. A_deaminase. [Tree]
InterPro	IPR006650. A/AMP_deam_AS. IPR001365. A/AMP_deaminase_dom. IPR006330. A_deaminase. [Graphical view]
KO	K01488.
PANTHER	PTHR11409:SF21. PTHR11409:SF21. 1 hit.
Pfam	PF00962. A_deaminase. 1 hit. [Graphical view]
TIGRFAMs	TIGR01430. Aden_deam. 1 hit.
PROSITE	PS00485. A_DEAMINASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ADD_KLEP3

Accession

Primary (citable) accession number: B5XWQ7

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	November 25, 2008
Last modified:	January 25, 2012
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program