B5XW55 (KDSA_KLEP3) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
2-dehydro-3-deoxyphosphooctonate aldolase
EC=2.5.1.55

Alternative name(s):
3-deoxy-D-manno-octulosonic acid 8-phosphate synthase
KDO-8-phosphate synthase
Short name=KDO 8-P synthase
Short name=KDOPS
Phospho-2-dehydro-3-deoxyoctonate aldolase

Gene names

Name:	kdsA
Ordered Locus Names:	KPK_2073

Organism

Klebsiella pneumoniae (strain 342) [Complete proteome] [HAMAP]

Taxonomic identifier

507522 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Klebsiella

Protein attributes

Sequence length	284 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Phosphoenolpyruvate + D-arabinose 5-phosphate + H2O = 2-dehydro-3-deoxy-D-octonate 8-phosphate + phosphate. HAMAP MF_00056
Pathway	Carbohydrate biosynthesis; 3-deoxy-D-manno-octulosonate biosynthesis; 3-deoxy-D-manno-octulosonate from D-ribulose 5-phosphate: step 2/3. HAMAP MF_00056 Bacterial outer membrane biogenesis; lipopolysaccharide biosynthesis. HAMAP MF_00056
Subcellular location	Cytoplasm By similarity HAMAP MF_00056.
Sequence similarities	Belongs to the KdsA family.

Ontologies

Keywords
Biological process	Lipopolysaccharide biosynthesis
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	lipopolysaccharide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-deoxy-8-phosphooctulonate synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 284	284	2-dehydro-3-deoxyphosphooctonate aldolase HAMAP MF_00056		PRO_1000091821

Sequences

	Sequence		Length	Mass (Da)
	B5XW55 [UniParc]. Last modified November 25, 2008. Version 1. Checksum: 4AA0E1BDCAF210F3	FASTA	284	30,825
	10 20 30 40 50 60 MKQKVVSIGD INVANDLPFV LFGGMNVLES RDLAMRICEH YVTVTQKLGI PYVFKASFDK 70 80 90 100 110 120 ANRSSIHSYR GPGLEEGMKI FQELKQTFGV KIITDVHEAS QAQPVADVVD VIQLPAFLAR 130 140 150 160 170 180 QTDLVEAMAK TGAVINVKKP QFVSPGQMGN IVDKFIEGGN DKVILCDRGA NFGYDNLVVD 190 200 210 220 230 240 MLGFGVMKKA SNNSPVIFDV THALQCRDPF GAASGGRRAQ VSELARAGMA VGIAGLFIEA 250 260 270 280 HPDPDHAKCD GPSALPLDKL EPFLKQMKAI DDLVKSFDEL DTSK

References

[1]

"Complete genome sequence of the N2-fixing broad host range endophyte Klebsiella pneumoniae 342 and virulence predictions verified in mice." Fouts D.E., Tyler H.L., DeBoy R.T., Daugherty S., Ren Q., Badger J.H., Durkin A.S., Huot H., Shrivastava S., Kothari S., Dodson R.J., Mohamoud Y., Khouri H., Roesch L.F.W., Krogfelt K.A., Struve C., Triplett E.W., Methe B.A. PLoS Genet. 4:E1000141-E1000141(2008) [PubMed: 18654632] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 342.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000964 Genomic DNA. Translation: ACI08156.1.
RefSeq	YP_002237912.1. NC_011283.1.
3D structure databases
ProteinModelPortal	B5XW55.
SMR	B5XW55. Positions 1-284.
ModBase	Search...
Protein-protein interaction databases
STRING	B5XW55.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6937080.
GenomeReviews	Gene locus KPK_2073 in contig CP000964_GR.
KEGG	kpe:KPK_2073.
PATRIC	20437630. VBIKlePne121904_2193.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG575990.
OMA	QLILCDR.
ProtClustDB	PRK05198.
Family and domain databases
HAMAP	MF_00056. KDO8P_synth. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR006218. DAHP1/KDSA. IPR006269. KDO8P_synthase. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
KO	K01627.
PANTHER	PTHR21057:SF2. KDO8P_synthase. 1 hit.
Pfam	PF00793. DAHP_synth_1. 1 hit. [Graphical view]
TIGRFAMs	TIGR01362. KDO8P_synth. 1 hit.
ProtoNet	Search...

Entry information

Entry name

KDSA_KLEP3

Accession

Primary (citable) accession number: B5XW55

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	November 25, 2008
Last modified:	January 25, 2012
This is version 22 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program