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Protein

Glutamate--tRNA ligase

Gene

gltX

Organism
Klebsiella pneumoniae (strain 342)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).UniRule annotation

Catalytic activityi

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi98ZincUniRule annotation1
Metal bindingi100ZincUniRule annotation1
Metal bindingi125ZincUniRule annotation1
Metal bindingi127ZincUniRule annotation1
Binding sitei240ATPUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate--tRNA ligaseUniRule annotation (EC:6.1.1.17UniRule annotation)
Alternative name(s):
Glutamyl-tRNA synthetaseUniRule annotation
Short name:
GluRSUniRule annotation
Gene namesi
Name:gltXUniRule annotation
Ordered Locus Names:KPK_1389
OrganismiKlebsiella pneumoniae (strain 342)
Taxonomic identifieri507522 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeKlebsiella
Proteomesi
  • UP000001734 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000900811 – 472Glutamate--tRNA ligaseAdd BLAST472

Interactioni

Subunit structurei

Monomer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliB5XVT8
SMRiB5XVT8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi9 – 19"HIGH" regionUniRule annotationAdd BLAST11
Motifi237 – 241"KMSKS" regionUniRule annotation5

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. Glutamate--tRNA ligase type 1 subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000252722
KOiK01885
OMAiHYINTLP

Family and domain databases

CDDicd00808 GluRS_core, 1 hit
Gene3Di1.10.10.350, 1 hit
3.40.50.620, 1 hit
HAMAPiMF_00022 Glu_tRNA_synth_type1, 1 hit
InterProiView protein in InterPro
IPR008925 aa-tRNA-synth_I_codon-bd
IPR020751 aa-tRNA-synth_I_codon-bd_sub2
IPR001412 aa-tRNA-synth_I_CS
IPR004527 Glu-tRNA-ligase_bac/mito
IPR000924 Glu/Gln-tRNA-synth
IPR020058 Glu/Gln-tRNA-synth_Ib_cat-dom
IPR033910 GluRS_core
IPR014729 Rossmann-like_a/b/a_fold
PfamiView protein in Pfam
PF00749 tRNA-synt_1c, 1 hit
PRINTSiPR00987 TRNASYNTHGLU
SUPFAMiSSF48163 SSF48163, 1 hit
TIGRFAMsiTIGR00464 gltX_bact, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

B5XVT8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKIKTRFAPS PTGYLHVGGA RTALYSWLFA RNHGGEFVLR IEDTDLERST
60 70 80 90 100
PEAIEAIMDG MNWLNLQWDE GPYYQTKRFD RYNNVIDEML EAGTAYKCYC
110 120 130 140 150
SKERLEALRE EQMANGEKPR YDGRCRHSHE HHADDEPCVV RFANPQEGSV
160 170 180 190 200
IFDDQIRGPI EFSNQELDDL IIRRTDGSPT YNFCVVVDDW DMAITHVIRG
210 220 230 240 250
EDHINNTPRQ INILKALNAP VPVYAHVSMI NGDDGKKLSK RHGAVSVMQY
260 270 280 290 300
RDDGYLPEAL LNYLVRLGWS HGDQEIFTRE EMIEFFSLGA VSKSASAFNT
310 320 330 340 350
DKLLWLNHHY INTLPAEYVA THLQWHIEQE NIDTRNGPQL AELVKLLGER
360 370 380 390 400
CKTLKEIAQS CRYFYEEFAE FDADAAKKHL RPVARQPLEV VRDKLAAIVD
410 420 430 440 450
WTAENVHHAI QATADELEVG MGKVAMPLRV AVTGAGQSRA LDVTVHAIGK
460 470
SRSVERINKA LAFIAEREGQ AS
Length:472
Mass (Da):53,655
Last modified:November 25, 2008 - v1
Checksum:i43D3045213C08D4B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000964 Genomic DNA Translation: ACI08095.1
RefSeqiWP_012540948.1, NC_011283.1

Genome annotation databases

EnsemblBacteriaiACI08095; ACI08095; KPK_1389
KEGGikpe:KPK_1389

Similar proteinsi

Entry informationi

Entry nameiSYE_KLEP3
AccessioniPrimary (citable) accession number: B5XVT8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: November 25, 2008
Last modified: March 28, 2018
This is version 66 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

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