Reviewed, UniProtKB/Swiss-Prot B5XVR1 (HEM6_KLEP3)

Names and origin

Protein names

Recommended name:
    Coproporphyrinogen-III oxidase, aerobic
      Short name=Coproporphyrinogenase
      Short name=Coprogen oxidase
    EC=1.3.3.3

Gene names

Name:	hemF
Ordered Locus Names:	KPK_1358

Organism

Klebsiella pneumoniae (strain 342) [Complete proteome] [HAMAP]

Taxonomic identifier

507522 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Klebsiella

Protein attributes

Sequence length	299 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Key enzyme in heme biosynthesis. Catalyzes the oxidative decarboxylation of propionic acid side chains of rings A and B of coproporphyrinogen III By similarity. HAMAP MF_00333
Catalytic activity	Coproporphyrinogen-III + O2 + 2 H+ = protoporphyrinogen-IX + 2 CO2 + 2 H2O. HAMAP MF_00333
Pathway	Porphyrin metabolism; protoporphyrin-IX biosynthesis; protoporphyrinogen-IX from coproporphyrinogen-III (O2 route): step 1/1. HAMAP MF_00333
Subunit structure	Homodimer By similarity. HAMAP MF_00333
Subcellular location	Cytoplasm By similarity HAMAP MF_00333.
Sequence similarities	Belongs to the aerobic coproporphyrinogen-III oxidase family.

Ontologies

Keywords
Biological process	Porphyrin biosynthesis
Cellular component	Cytoplasm
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW porphyrin biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	coproporphyrinogen oxidase activity Inferred from electronic annotation. Source: HAMAP protein homodimerization activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 299	299	Coproporphyrinogen-III oxidase, aerobic HAMAP MF_00333		PRO_1000119805
Regions
	Region	48 – 57	10	Important for dimerization By similarity
	Region	108 – 110	3	Substrate binding By similarity
	Region	240 – 275	36	Important for dimerization By similarity
	Region	258 – 263	6	Substrate binding By similarity
Sites
	Active site	106	1	Proton donor By similarity
	Binding site	92	1	Substrate By similarity
	Site	175	1	Important for dimerization By similarity

Sequences

	Sequence		Length	Mass (Da)
	B5XVR1-1 [UniParc]. Last modified November 25, 2008. Version 1. Checksum: 20696F4DB4E46C9E	FASTA	299	34,227
	10 20 30 40 50 60 MKPDAAQVKT FLLQLQDNLC QQLSAVDGAP FIEDAWQREG GGGGRSRVLR DGNVFEQAGV 70 80 90 100 110 120 NFSHVHGDAM PASATAHRPE LAGRSFEAMG VSLVVHPLNP YVPTSHANVR FFIAEKPGAD 130 140 150 160 170 180 PVWWFGGGFD LTPYYGFEED AIHWHRTARD LCLPFGEEVY PRYKKWCDDY FYLKHRQEQR 190 200 210 220 230 240 GIGGLFFDDL NTPDFDHCFA FMQAVGNGYA DAYLPIVERR KAMPYGERER HFQLYRRGRY 250 260 270 280 290 VEFNLVWDRG TLFGLQTGGR TESILMSMPP LVRWEYDYQP EPGSPEAALS EFIQVRDWL

References

[1]

"Complete genome sequence of the N2-fixing broad host range endophyte Klebsiella pneumoniae 342 and virulence predictions verified in mice." Fouts D.E., Tyler H.L., DeBoy R.T., Daugherty S., Ren Q., Badger J.H., Durkin A.S., Huot H., Shrivastava S., Kothari S., Dodson R.J., Mohamoud Y., Khouri H., Roesch L.F.W., Krogfelt K.A., Struve C., Triplett E.W., Methe B.A. PLoS Genet. 4:E1000141-E1000141(2008) [PubMed: 18654632] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000964 Genomic DNA. Translation: ACI11085.1.
RefSeq	YP_002237218.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	6938553.
GenomeReviews	Gene locus KPK_1358 in contig CP000964_GR.
KEGG	kpe:KPK_1358.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG631180.
OMA	HMNTRFV.
Family and domain databases
HAMAP	MF_00333. Coprogen_oxidas. [Tree]
InterPro	IPR001260. Coprogen_oxidas. IPR018375. Coproporphyrinogen-3_ox_CS. [Graphical view]
Gene3D	G3DSA:3.40.1500.10. Coprogen_oxidas. 1 hit.
PANTHER	PTHR10755. Coprogen_oxidas. 1 hit.
Pfam	PF01218. Coprogen_oxidas. 1 hit. [Graphical view]
PIRSF	PIRSF000166. Coproporphyri_ox. 1 hit.
PRINTS	PR00073. COPRGNOXDASE.
PROSITE	PS01021. COPROGEN_OXIDASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HEM6_KLEP3

Accession

Primary (citable) accession number: B5XVR1

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	November 25, 2008
Last modified:	January 19, 2010
This is version 15 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)