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Protein

NAD kinase

Gene

nadK

Organism
Klebsiella pneumoniae (strain 342)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the regulation of the intracellular balance of NAD and NADP, and is a key enzyme in the biosynthesis of NADP. Catalyzes specifically the phosphorylation on 2'-hydroxyl of the adenosine moiety of NAD to yield NADP.UniRule annotation

Catalytic activityi

ATP + NAD+ = ADP + NADP+.UniRule annotation

Cofactori

a divalent metal cationUniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei73 – 731Proton acceptorUniRule annotation
Binding sitei158 – 1581NADUniRule annotation
Binding sitei175 – 1751NADUniRule annotation
Binding sitei177 – 1771NADUniRule annotation
Binding sitei247 – 2471NADUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi73 – 742NADUniRule annotation
Nucleotide bindingi147 – 1482NADUniRule annotation
Nucleotide bindingi188 – 1936NADUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-HAMAP
  3. NAD+ kinase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. NAD metabolic process Source: InterPro
  2. NADP biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, NAD, NADP, Nucleotide-binding

Enzyme and pathway databases

BioCyciKPNE507522:GI0B-1184-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
NAD kinaseUniRule annotation (EC:2.7.1.23UniRule annotation)
Alternative name(s):
ATP-dependent NAD kinaseUniRule annotation
Gene namesi
Name:nadKUniRule annotation
Ordered Locus Names:KPK_1183
OrganismiKlebsiella pneumoniae (strain 342)
Taxonomic identifieri507522 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeKlebsiella
ProteomesiUP000001734: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 292292NAD kinasePRO_1000120869Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi507522.KPK_1183.

Structurei

3D structure databases

ProteinModelPortaliB5XVJ8.
SMRiB5XVJ8. Positions 4-292.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the NAD kinase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0061.
HOGENOMiHOG000227221.
KOiK00858.
OMAiGMVELRV.
OrthoDBiEOG6PZXDR.

Family and domain databases

Gene3Di2.60.200.30. 1 hit.
3.40.50.10330. 1 hit.
HAMAPiMF_00361. NAD_kinase.
InterProiIPR017438. ATP-NAD_kinase_dom_1.
IPR016064. ATP-NAD_kinase_PpnK-typ.
IPR017437. ATP-NAD_kinase_PpnK-typ_all-b.
IPR002504. PolyP/ATP_NADK.
[Graphical view]
PANTHERiPTHR20275. PTHR20275. 1 hit.
PfamiPF01513. NAD_kinase. 1 hit.
[Graphical view]
SUPFAMiSSF111331. SSF111331. 1 hit.

Sequencei

Sequence statusi: Complete.

B5XVJ8-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKNHFKCIGI VGHPRHPTAL TTHEMLWRWL CSKGYEVLVE QQIAHELQLS
60 70 80 90 100
NVKTGTLAEI GQQADLAVVV GGDGNMLGAA RTLARYDINV IGINRGNLGF
110 120 130 140 150
LTDLDPDNAL QQLADVLEGH YIAEKRFLLE AQVCQQDCQK RISTAINEVV
160 170 180 190 200
LHPGKVAHMI EFEVYIDEVF AFSQRSDGLI ISTPTGSTAY SLSAGGPILT
210 220 230 240 250
PSLDAITLVP MFPHTLSARP LVINGDSTIR LRFSHRCSDL EISCDSQIAL
260 270 280 290
PIQDGEDVLI RRCDYHLNLI HPKDYSYFNT LSTKLGWSKK LF
Length:292
Mass (Da):32,408
Last modified:November 25, 2008 - v1
Checksum:iFCF10DF2792D2C7C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000964 Genomic DNA. Translation: ACI07454.1.
RefSeqiYP_002237047.1. NC_011283.1.

Genome annotation databases

EnsemblBacteriaiACI07454; ACI07454; KPK_1183.
GeneIDi6938939.
KEGGikpe:KPK_1183.
PATRICi20435933. VBIKlePne121904_1365.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000964 Genomic DNA. Translation: ACI07454.1.
RefSeqiYP_002237047.1. NC_011283.1.

3D structure databases

ProteinModelPortaliB5XVJ8.
SMRiB5XVJ8. Positions 4-292.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi507522.KPK_1183.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACI07454; ACI07454; KPK_1183.
GeneIDi6938939.
KEGGikpe:KPK_1183.
PATRICi20435933. VBIKlePne121904_1365.

Phylogenomic databases

eggNOGiCOG0061.
HOGENOMiHOG000227221.
KOiK00858.
OMAiGMVELRV.
OrthoDBiEOG6PZXDR.

Enzyme and pathway databases

BioCyciKPNE507522:GI0B-1184-MONOMER.

Family and domain databases

Gene3Di2.60.200.30. 1 hit.
3.40.50.10330. 1 hit.
HAMAPiMF_00361. NAD_kinase.
InterProiIPR017438. ATP-NAD_kinase_dom_1.
IPR016064. ATP-NAD_kinase_PpnK-typ.
IPR017437. ATP-NAD_kinase_PpnK-typ_all-b.
IPR002504. PolyP/ATP_NADK.
[Graphical view]
PANTHERiPTHR20275. PTHR20275. 1 hit.
PfamiPF01513. NAD_kinase. 1 hit.
[Graphical view]
SUPFAMiSSF111331. SSF111331. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Complete genome sequence of the N2-fixing broad host range endophyte Klebsiella pneumoniae 342 and virulence predictions verified in mice."
    Fouts D.E., Tyler H.L., DeBoy R.T., Daugherty S., Ren Q., Badger J.H., Durkin A.S., Huot H., Shrivastava S., Kothari S., Dodson R.J., Mohamoud Y., Khouri H., Roesch L.F.W., Krogfelt K.A., Struve C., Triplett E.W., Methe B.A.
    PLoS Genet. 4:E1000141-E1000141(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 342.

Entry informationi

Entry nameiNADK_KLEP3
AccessioniPrimary (citable) accession number: B5XVJ8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: November 25, 2008
Last modified: January 7, 2015
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.