B5XV37 (SURE_KLEP3) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
5'/3'-nucleotidase SurE
EC=3.1.3.5
EC=3.1.3.6

Alternative name(s):
Exopolyphosphatase
EC=3.6.1.11
Nucleoside monophosphate phosphohydrolase

Gene names

Name:	surE
Ordered Locus Names:	KPK_1017

Organism

Klebsiella pneumoniae (strain 342) [Complete proteome] [HAMAP]

Taxonomic identifier

507522 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Klebsiella › Klebsiella pneumoniae

Protein attributes

Sequence length	253 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Nucleotidase with a broad substrate specificity as it can dephosphorylate various ribo- and deoxyribonucleoside 5'-monophosphates and ribonucleoside 3'-monophosphates with highest affinity to 3'-AMP. Also hydrolyzes polyphosphate (exopolyphosphatase activity) with the preference for short-chain-length substrates (P20-25). Might be involved in the regulation of dNTP and NTP pools, and in the turnover of 3'-mononucleotides produced by numerous intracellular RNases (T1, T2, and F) during the degradation of various RNAs By similarity. HAMAP-Rule MF_00060
Catalytic activity	A 5'-ribonucleotide + H2O = a ribonucleoside + phosphate. HAMAP-Rule MF_00060 A 3'-ribonucleotide + H2O = a ribonucleoside + phosphate. HAMAP-Rule MF_00060 (Polyphosphate)(n) + H2O = (polyphosphate)(n-1) + phosphate. HAMAP-Rule MF_00060
Cofactor	Binds 1 divalent metal cation per subunit By similarity.
Subcellular location	Cytoplasm Potential HAMAP-Rule MF_00060.
Sequence similarities	Belongs to the SurE nucleotidase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Metal-binding Nucleotide-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	3'-nucleotidase activity Inferred from electronic annotation. Source: HAMAP 5'-nucleotidase activity Inferred from electronic annotation. Source: HAMAP exopolyphosphatase activity Inferred from electronic annotation. Source: HAMAP metal ion binding Inferred from electronic annotation. Source: HAMAP nucleotide binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 253	253	5'/3'-nucleotidase SurE HAMAP-Rule MF_00060		PRO_1000092012
Sites
	Metal binding	8	1	Divalent metal cation By similarity
	Metal binding	9	1	Divalent metal cation By similarity
	Metal binding	39	1	Divalent metal cation By similarity
	Metal binding	92	1	Divalent metal cation By similarity

Sequences

	Sequence		Length	Mass (Da)
	B5XV37 [UniParc]. Last modified November 25, 2008. Version 1. Checksum: 680C1579099943F0	FASTA	253	27,252
	10 20 30 40 50 60 MRILLSNDDG IHAPGIQTLA KALREFAEVQ VVAPDRNRSG ASNSLTLESS LRTFTFENGD 70 80 90 100 110 120 IAVQMGTPTD CVYLGVNALM RPRPDIVVSG INAGPNLGDD VIYSGTVAAA MEGRHLGFPA 130 140 150 160 170 180 LAVSLNGYQH YDTAAAVTCT ILRALSREPL RTGRILNINV PDLPLDQIKG IRVTRCGNRH 190 200 210 220 230 240 PADQVIPQKD PRGNTLYWIG PPGDKRDAGP GTDFAAVDEG YVSVTPLHVD LTAHQAHEVV 250 SDWLERVGVD GQW

References

[1]

"Complete genome sequence of the N2-fixing broad host range endophyte Klebsiella pneumoniae 342 and virulence predictions verified in mice." Fouts D.E., Tyler H.L., DeBoy R.T., Daugherty S., Ren Q., Badger J.H., Durkin A.S., Huot H., Shrivastava S., Kothari S., Dodson R.J., Mohamoud Y., Khouri H., Roesch L.F.W., Krogfelt K.A., Struve C., Triplett E.W., Methe B.A. PLoS Genet. 4:E1000141-E1000141(2008) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 342.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000964 Genomic DNA. Translation: ACI11010.1.
RefSeq	YP_002236886.1. NC_011283.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	507522.KPK_1017.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACI11010; ACI11010; KPK_1017.
GeneID	6937264.
KEGG	kpe:KPK_1017.
PATRIC	20435611. VBIKlePne121904_1210.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0496.
HOGENOM	HOG000122500.
KO	K03787.
OMA	LGSNMGE.
ProtClustDB	PRK00346.
Enzyme and pathway databases
BioCyc	KPNE507522:GI0B-4489-MONOMER.
Family and domain databases
Gene3D	3.40.1210.10. 1 hit.
HAMAP	MF_00060. SurE.
InterPro	IPR002828. SurE-like_Pase/nucleotidase. [Graphical view]
Pfam	PF01975. SurE. 1 hit. [Graphical view]
SUPFAM	SSF64167. SurE-like_Pase/nucleotidase. 1 hit.
TIGRFAMs	TIGR00087. surE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

SURE_KLEP3

Accession

Primary (citable) accession number: B5XV37

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	November 25, 2008
Last modified:	May 1, 2013
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program