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B5XUQ2 (B5XUQ2_KLEP3) Unreviewed, UniProtKB/TrEMBL

Last modified June 11, 2014. Version 43. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry infoCustomize order

Names and origin

Protein namesRecommended name:
Thymidylate synthase HAMAP-Rule MF_00008

Short name=TS HAMAP-Rule MF_00008
Short name=TSase HAMAP-Rule MF_00008
EC=2.1.1.45 HAMAP-Rule MF_00008
Gene names
Name:thyA HAMAP-Rule MF_00008 EMBL ACI08665.1
Ordered Locus Names:KPK_0879 EMBL ACI08665.1
OrganismKlebsiella pneumoniae (strain 342) [Complete proteome] [HAMAP] EMBL ACI08665.1
Taxonomic identifier507522 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeKlebsiella

Protein attributes

Sequence length264 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Provides the sole de novo source of dTMP for DNA biosynthesis By similarity. HAMAP-Rule MF_00008

Catalytic activity

5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP. HAMAP-Rule MF_00008 SAAS SAAS000398

Pathway

Pyrimidine metabolism; dTTP biosynthesis. HAMAP-Rule MF_00008 SAAS SAAS000398

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_00008 SAAS SAAS000398

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00008.

Sequence similarities

Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily. HAMAP-Rule MF_00008

Ontologies

Keywords
   Biological processNucleotide biosynthesis HAMAP-Rule MF_00008 SAAS SAAS000398
   Cellular componentCytoplasm HAMAP-Rule MF_00008
   Molecular functionMethyltransferase HAMAP-Rule MF_00008 SAAS SAAS000398 EMBL ACI08665.1
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processdTMP biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-HAMAP

dTTP biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionthymidylate synthase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Sites

Active site1461 By similarity HAMAP-Rule MF_00008

Sequences

Sequence LengthMass (Da)Tools
B5XUQ2 [UniParc].

Last modified November 25, 2008. Version 1.
Checksum: A405AF51A800ED6E

FASTA26430,293
        10         20         30         40         50         60 
MKQYLDLMQK VLTEGTPKND RTGTGTISIF GHQMRFNLQE GFPLVTTKRC HLRSIIHELL 

        70         80         90        100        110        120 
WFLQGDTNIA YLHDNNVTIW DEWADENGDL GPVYGKQWRS WPAPDGRHID QITTVMNQLK 

       130        140        150        160        170        180 
NDPDSRRIIV SAWNVGELDK MALAPCHAFF QFYVADGKLS CQLYQRSCDV FLGLPFNIAS 

       190        200        210        220        230        240 
YALLVHMVAQ QCDLQVGDFV WTGGDTHLYS NHLEQTNLQL SREPRPLPKL VIKRKPASIF 

       250        260 
DYRFEDFEIE GYDPHPGIKA PVAI 

« Hide

References

[1]"Complete genome sequence of the N2-fixing broad host range endophyte Klebsiella pneumoniae 342 and virulence predictions verified in mice."
Fouts D.E., Tyler H.L., DeBoy R.T., Daugherty S., Ren Q., Badger J.H., Durkin A.S., Huot H., Shrivastava S., Kothari S., Dodson R.J., Mohamoud Y., Khouri H., Roesch L.F., Krogfelt K.A., Struve C., Triplett E.W., Methe B.A.
PLoS Genet. 4:E1000141-E1000141(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 342 EMBL ACI08665.1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000964 Genomic DNA. Translation: ACI08665.1.
RefSeqYP_002236751.1. NC_011283.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING507522.KPK_0879.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACI08665; ACI08665; KPK_0879.
GeneID6936400.
KEGGkpe:KPK_0879.
PATRIC20435338. VBIKlePne121904_1077.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0207.
HOGENOMHOG000257899.
KOK00560.
OMADVHLYSN.
OrthoDBEOG6K6V53.

Enzyme and pathway databases

BioCycKPNE507522:GI0B-880-MONOMER.
UniPathwayUPA00575.

Family and domain databases

Gene3D3.30.572.10. 1 hit.
HAMAPMF_00008. Thymidy_synth_bact.
InterProIPR023451. Thymidate_synth/dCMP_Mease.
IPR000398. Thymidylate_synthase.
IPR020940. Thymidylate_synthase_AS.
[Graphical view]
PfamPF00303. Thymidylat_synt. 1 hit.
[Graphical view]
PRINTSPR00108. THYMDSNTHASE.
SUPFAMSSF55831. SSF55831. 1 hit.
TIGRFAMsTIGR03284. thym_sym. 2 hits.
PROSITEPS00091. THYMIDYLATE_SYNTHASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameB5XUQ2_KLEP3
AccessionPrimary (citable) accession number: B5XUQ2
Entry history
Integrated into UniProtKB/TrEMBL: November 25, 2008
Last sequence update: November 25, 2008
Last modified: June 11, 2014
This is version 43 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)