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Protein

Nucleoid occlusion factor SlmA

Gene

slmA

Organism
Klebsiella pneumoniae (strain 342)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Required for nucleoid occlusion (NO) phenomenon, which prevents Z-ring formation and cell division over the nucleoid. Acts as a DNA-associated cell division inhibitor that binds simultaneously chromosomal DNA and FtsZ, and disrupts the assembly of FtsZ polymers. SlmA-DNA-binding sequences (SBS) are dispersed on non-Ter regions of the chromosome, preventing FtsZ polymerization at these regions.UniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi33 – 5220H-T-H motifUniRule annotationAdd
BLAST

GO - Molecular functioni

  1. sequence-specific DNA binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. cell cycle Source: UniProtKB-KW
  2. cell division Source: UniProtKB-KW
  3. negative regulation of barrier septum assembly Source: InterPro
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciKPNE507522:GI0B-113-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Nucleoid occlusion factor SlmAUniRule annotation
Gene namesi
Name:slmAUniRule annotation
Ordered Locus Names:KPK_0113
OrganismiKlebsiella pneumoniae (strain 342)
Taxonomic identifieri507522 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeKlebsiella
ProteomesiUP000001734 Componenti: Chromosome

Subcellular locationi

  1. Cytoplasmnucleoid UniRule annotation

GO - Cellular componenti

  1. bacterial nucleoid Source: UniProtKB-HAMAP
  2. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 198198Nucleoid occlusion factor SlmAPRO_1000188391Add
BLAST

Interactioni

Subunit structurei

Homodimer. Interacts with FtsZ.UniRule annotation

Protein-protein interaction databases

STRINGi507522.KPK_0113.

Structurei

Secondary structure

1
198
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi10 – 2415Combined sources
Helixi26 – 294Combined sources
Helixi34 – 418Combined sources
Helixi45 – 517Combined sources
Helixi55 – 8026Combined sources
Helixi84 – 10118Combined sources
Helixi103 – 1097Combined sources
Helixi113 – 1164Combined sources
Helixi119 – 14729Combined sources
Helixi155 – 17521Combined sources
Turni176 – 1783Combined sources
Turni182 – 1854Combined sources
Helixi186 – 1949Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4GCKX-ray2.05A/B/C/D1-198[»]
4GFLX-ray2.30A/B1-198[»]
ProteinModelPortaliB5XTG2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini10 – 7061HTH tetR-typeUniRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili119 – 14426UniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the nucleoid occlusion factor SlmA family.UniRule annotation
Contains 1 HTH tetR-type DNA-binding domain.UniRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiCOG1309.
HOGENOMiHOG000266053.
KOiK05501.
OMAiEKDTSAR.
OrthoDBiEOG6G7R3C.

Family and domain databases

Gene3Di1.10.357.10. 1 hit.
HAMAPiMF_01839. NO_factor_SlmA.
InterProiIPR023772. DNA-bd_HTH_TetR-type_CS.
IPR009057. Homeodomain-like.
IPR001647. HTH_TetR.
IPR023769. NO_SlmA.
IPR015893. Tet_transcr_reg_TetR-like_C.
IPR011075. Tet_transcr_reg_TetR-rel_C.
[Graphical view]
PfamiPF00440. TetR_N. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
SSF48498. SSF48498. 1 hit.
PROSITEiPS01081. HTH_TETR_1. 1 hit.
PS50977. HTH_TETR_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B5XTG2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAEKQTAKRN RREEILQSLA LMLESSDGSQ RITTAKLAAS VGVSEAALYR
60 70 80 90 100
HFPSKTRMFD SLIEFIEDSL ITRINLILKD EKDTTARLRL IVLLILGFGE
110 120 130 140 150
RNPGLTRILT GHALMFEQDR LQGRINQLFE RIEVQLRQVM REKKMREGEG
160 170 180 190
YTLDETLLAS QLLAFCEGML SRFVRSEFKY RPTDDFEARW PLVAAQLQ
Length:198
Mass (Da):22,866
Last modified:November 25, 2008 - v1
Checksum:iBC44ACAC8F5023AA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000964 Genomic DNA. Translation: ACI06851.1.
RefSeqiYP_002235997.1. NC_011283.1.

Genome annotation databases

EnsemblBacteriaiACI06851; ACI06851; KPK_0113.
KEGGikpe:KPK_0113.
PATRICi20433836. VBIKlePne121904_0338.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000964 Genomic DNA. Translation: ACI06851.1.
RefSeqiYP_002235997.1. NC_011283.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4GCKX-ray2.05A/B/C/D1-198[»]
4GFLX-ray2.30A/B1-198[»]
ProteinModelPortaliB5XTG2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi507522.KPK_0113.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACI06851; ACI06851; KPK_0113.
KEGGikpe:KPK_0113.
PATRICi20433836. VBIKlePne121904_0338.

Phylogenomic databases

eggNOGiCOG1309.
HOGENOMiHOG000266053.
KOiK05501.
OMAiEKDTSAR.
OrthoDBiEOG6G7R3C.

Enzyme and pathway databases

BioCyciKPNE507522:GI0B-113-MONOMER.

Family and domain databases

Gene3Di1.10.357.10. 1 hit.
HAMAPiMF_01839. NO_factor_SlmA.
InterProiIPR023772. DNA-bd_HTH_TetR-type_CS.
IPR009057. Homeodomain-like.
IPR001647. HTH_TetR.
IPR023769. NO_SlmA.
IPR015893. Tet_transcr_reg_TetR-like_C.
IPR011075. Tet_transcr_reg_TetR-rel_C.
[Graphical view]
PfamiPF00440. TetR_N. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
SSF48498. SSF48498. 1 hit.
PROSITEiPS01081. HTH_TETR_1. 1 hit.
PS50977. HTH_TETR_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Complete genome sequence of the N2-fixing broad host range endophyte Klebsiella pneumoniae 342 and virulence predictions verified in mice."
    Fouts D.E., Tyler H.L., DeBoy R.T., Daugherty S., Ren Q., Badger J.H., Durkin A.S., Huot H., Shrivastava S., Kothari S., Dodson R.J., Mohamoud Y., Khouri H., Roesch L.F.W., Krogfelt K.A., Struve C., Triplett E.W., Methe B.A.
    PLoS Genet. 4:E1000141-E1000141(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 342.

Entry informationi

Entry nameiSLMA_KLEP3
AccessioniPrimary (citable) accession number: B5XTG2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: November 25, 2008
Last modified: April 1, 2015
This is version 48 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.