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B5XQY2

- BGAL_KLEP3

UniProt

B5XQY2 - BGAL_KLEP3

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Protein

Beta-galactosidase

Gene
lacZ, KPK_2776
Organism
Klebsiella pneumoniae (strain 342)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.UniRule annotation

Cofactori

Binds 2 magnesium ions per monomer By similarity.UniRule annotation
Binds 1 sodium ion per monomer By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei109 – 1091Substrate By similarity
Metal bindingi208 – 2081Sodium By similarity
Binding sitei208 – 2081Substrate By similarity
Sitei365 – 3651Transition state stabilizer By similarity
Sitei399 – 3991Transition state stabilizer By similarity
Metal bindingi424 – 4241Magnesium 1 By similarity
Metal bindingi426 – 4261Magnesium 1 By similarity
Active sitei469 – 4691Proton donor By similarity
Metal bindingi469 – 4691Magnesium 1 By similarity
Binding sitei469 – 4691Substrate By similarity
Active sitei545 – 5451Nucleophile By similarity
Metal bindingi605 – 6051Magnesium 2 By similarity
Metal bindingi609 – 6091Sodium; via carbonyl oxygen By similarity
Metal bindingi612 – 6121Sodium By similarity
Binding sitei612 – 6121Substrate By similarity
Binding sitei1011 – 10111Substrate By similarity

GO - Molecular functioni

  1. beta-galactosidase activity Source: UniProtKB-EC
  2. carbohydrate binding Source: InterPro
  3. magnesium ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Ligandi

Magnesium, Metal-binding, Sodium

Enzyme and pathway databases

BioCyciKPNE507522:GI0B-2777-MONOMER.

Protein family/group databases

CAZyiGH2. Glycoside Hydrolase Family 2.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-galactosidase (EC:3.2.1.23)
Short name:
Beta-gal
Alternative name(s):
Lactase
Gene namesi
Name:lacZ
Ordered Locus Names:KPK_2776
OrganismiKlebsiella pneumoniae (strain 342)
Taxonomic identifieri507522 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeKlebsiella
ProteomesiUP000001734: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. beta-galactosidase complex Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10351035Beta-galactosidaseUniRule annotationPRO_0000367000Add
BLAST

Interactioni

Subunit structurei

Homotetramer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi507522.KPK_2776.

Structurei

3D structure databases

ProteinModelPortaliB5XQY2.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni545 – 5484Substrate binding By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG3250.
HOGENOMiHOG000252443.
KOiK01190.
OMAiQGINKPG.
OrthoDBiEOG6XWV0T.

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
2.60.40.320. 2 hits.
2.70.98.10. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_01687. Beta_gal.
InterProiIPR004199. B-gal_small/dom_5.
IPR011013. Gal_mutarotase_SF_dom.
IPR008979. Galactose-bd-like.
IPR014718. Glyco_hydro-type_carb-bd_sub.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR023933. Glyco_hydro_2_beta_Galsidase.
IPR023230. Glyco_hydro_2_CS.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF02929. Bgal_small_N. 1 hit.
PF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
PRINTSiPR00132. GLHYDRLASE2.
SMARTiSM01038. Bgal_small_N. 1 hit.
[Graphical view]
SUPFAMiSSF49303. SSF49303. 2 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
SSF74650. SSF74650. 1 hit.
PROSITEiPS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B5XQY2-1 [UniParc]FASTAAdd to Basket

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MQISDTGHSH MPDFHAVLAR EDWQNQTITH LNRLPAHPAF ASWRDELAAR     50
DNHLSTRRRQ LDGEWQFVYA RSPFAVDAQW LTQDLPDSRG TPVPSNWQME 100
GYDAPIYTNV RYPIDTTPPR VPEDNPTGCY SLHFTVEDTW RENGQTQIIF 150
DGVNSAFHLW CNGVWVGYSQ DSRLPAAFDL SPFLRPGDNR LCVMVMRWSA 200
GSWLEDQDMW RMSGIFRSVW LLNKPQQRLC DVQLTPALDA LYRDGTLQVQ 250
ATVEATEAAL AGLSVGVSLW RGEEQVAAGR QPLGTPAVDE RGHYAERVDF 300
ALAVTAPAHW SAETPNCYRA VVTLWRGDEL LEAEAWDIGF RRIEIADGLL 350
RLNGKPLLIR GVNRHEHHYL RGQVVTEADM VQDILLMKQN NFNAVRCSHY 400
PNAPRWYELC NRYGLYVVDE ANIETHGMVP MNRLSDDPAW LPAFSARVTR 450
MVQSNRNHPC IIIWSLGNES GGGGNHEALY HWLKRNDPSR PVQYEGGGAD 500
TTATDIICPM YARVERDQPI PAVPKWGIKK WISLPGEQRP LILCEYAHAM 550
GNSLGNFADY WQAFREYPRL QGGFIWDWAD QAIRKIFDDG SVGWAYGGDF 600
GDKPNDRQFC MNGLVFPDRT PHPSLVEAKH AQQYFQFTLL STSPLRVRIT 650
SEYLFRPTDN EVVRWQVQSA GETLYHGNLT LALPPEGSDE ITLLDSLILP 700
EGARAVWLTL EVTQPRATDW SEADHRVAWQ QFPLPAPLAL PAPTVPAGAP 750
DLIVSDEVWQ IRAGSQCWTI DRRTGLLSRW SVGGQEQLLT PLRDQFIRAP 800
LDNDIGVSEV ERIDPNAWVE RWKSAGLYDL EAHCVQCDAQ RLANETLVDC 850
RWHYLRGEEV VIVSHWRMHF TADGTLRLAV DGERAETLPP LPRVGLHFQV 900
ADQQAPVSWL GLGPHENYPD RRSSACFARW EQPLAAMTTP YIFPTENGLR 950
CDTQALDWGR WHVSGHFHFS VQPWSTRQLM ETDHWHKMQA EDGVWITLDG 1000
LHMGVGGDDS WTPSVLPQWL LSQTRWQYEF FLRCL 1035
Length:1,035
Mass (Da):117,917
Last modified:November 25, 2008 - v1
Checksum:iA44AC3C1E52DFCB5
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000964 Genomic DNA. Translation: ACI08231.1.
RefSeqiYP_002238606.1. NC_011283.1.

Genome annotation databases

EnsemblBacteriaiACI08231; ACI08231; KPK_2776.
GeneIDi6934703.
KEGGikpe:KPK_2776.
PATRICi20438958. VBIKlePne121904_2851.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000964 Genomic DNA. Translation: ACI08231.1 .
RefSeqi YP_002238606.1. NC_011283.1.

3D structure databases

ProteinModelPortali B5XQY2.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 507522.KPK_2776.

Protein family/group databases

CAZyi GH2. Glycoside Hydrolase Family 2.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACI08231 ; ACI08231 ; KPK_2776 .
GeneIDi 6934703.
KEGGi kpe:KPK_2776.
PATRICi 20438958. VBIKlePne121904_2851.

Phylogenomic databases

eggNOGi COG3250.
HOGENOMi HOG000252443.
KOi K01190.
OMAi QGINKPG.
OrthoDBi EOG6XWV0T.

Enzyme and pathway databases

BioCyci KPNE507522:GI0B-2777-MONOMER.

Family and domain databases

Gene3Di 2.60.120.260. 1 hit.
2.60.40.320. 2 hits.
2.70.98.10. 1 hit.
3.20.20.80. 1 hit.
HAMAPi MF_01687. Beta_gal.
InterProi IPR004199. B-gal_small/dom_5.
IPR011013. Gal_mutarotase_SF_dom.
IPR008979. Galactose-bd-like.
IPR014718. Glyco_hydro-type_carb-bd_sub.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR023933. Glyco_hydro_2_beta_Galsidase.
IPR023230. Glyco_hydro_2_CS.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
Pfami PF02929. Bgal_small_N. 1 hit.
PF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view ]
PRINTSi PR00132. GLHYDRLASE2.
SMARTi SM01038. Bgal_small_N. 1 hit.
[Graphical view ]
SUPFAMi SSF49303. SSF49303. 2 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
SSF74650. SSF74650. 1 hit.
PROSITEi PS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Complete genome sequence of the N2-fixing broad host range endophyte Klebsiella pneumoniae 342 and virulence predictions verified in mice."
    Fouts D.E., Tyler H.L., DeBoy R.T., Daugherty S., Ren Q., Badger J.H., Durkin A.S., Huot H., Shrivastava S., Kothari S., Dodson R.J., Mohamoud Y., Khouri H., Roesch L.F.W., Krogfelt K.A., Struve C., Triplett E.W., Methe B.A.
    PLoS Genet. 4:E1000141-E1000141(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 342.

Entry informationi

Entry nameiBGAL_KLEP3
AccessioniPrimary (citable) accession number: B5XQY2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: November 25, 2008
Last modified: May 14, 2014
This is version 40 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi