Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

B5XQY2 (BGAL_KLEP3) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 39. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Beta-galactosidase

Short name=Beta-gal
EC=3.2.1.23
Alternative name(s):
Lactase
Gene names
Name:lacZ
Ordered Locus Names:KPK_2776
OrganismKlebsiella pneumoniae (strain 342) [Complete proteome] [HAMAP]
Taxonomic identifier507522 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeKlebsiella

Protein attributes

Sequence length1035 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides. HAMAP-Rule MF_01687

Cofactor

Binds 2 magnesium ions per monomer By similarity. HAMAP-Rule MF_01687

Binds 1 sodium ion per monomer By similarity. HAMAP-Rule MF_01687

Subunit structure

Homotetramer By similarity. HAMAP-Rule MF_01687

Sequence similarities

Belongs to the glycosyl hydrolase 2 family.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 10351035Beta-galactosidase HAMAP-Rule MF_01687
PRO_0000367000

Regions

Region545 – 5484Substrate binding By similarity

Sites

Active site4691Proton donor By similarity
Active site5451Nucleophile By similarity
Metal binding2081Sodium By similarity
Metal binding4241Magnesium 1 By similarity
Metal binding4261Magnesium 1 By similarity
Metal binding4691Magnesium 1 By similarity
Metal binding6051Magnesium 2 By similarity
Metal binding6091Sodium; via carbonyl oxygen By similarity
Metal binding6121Sodium By similarity
Binding site1091Substrate By similarity
Binding site2081Substrate By similarity
Binding site4691Substrate By similarity
Binding site6121Substrate By similarity
Binding site10111Substrate By similarity
Site3651Transition state stabilizer By similarity
Site3991Transition state stabilizer By similarity

Sequences

Sequence LengthMass (Da)Tools
B5XQY2 [UniParc].

Last modified November 25, 2008. Version 1.
Checksum: A44AC3C1E52DFCB5

FASTA1,035117,917
        10         20         30         40         50         60 
MQISDTGHSH MPDFHAVLAR EDWQNQTITH LNRLPAHPAF ASWRDELAAR DNHLSTRRRQ 

        70         80         90        100        110        120 
LDGEWQFVYA RSPFAVDAQW LTQDLPDSRG TPVPSNWQME GYDAPIYTNV RYPIDTTPPR 

       130        140        150        160        170        180 
VPEDNPTGCY SLHFTVEDTW RENGQTQIIF DGVNSAFHLW CNGVWVGYSQ DSRLPAAFDL 

       190        200        210        220        230        240 
SPFLRPGDNR LCVMVMRWSA GSWLEDQDMW RMSGIFRSVW LLNKPQQRLC DVQLTPALDA 

       250        260        270        280        290        300 
LYRDGTLQVQ ATVEATEAAL AGLSVGVSLW RGEEQVAAGR QPLGTPAVDE RGHYAERVDF 

       310        320        330        340        350        360 
ALAVTAPAHW SAETPNCYRA VVTLWRGDEL LEAEAWDIGF RRIEIADGLL RLNGKPLLIR 

       370        380        390        400        410        420 
GVNRHEHHYL RGQVVTEADM VQDILLMKQN NFNAVRCSHY PNAPRWYELC NRYGLYVVDE 

       430        440        450        460        470        480 
ANIETHGMVP MNRLSDDPAW LPAFSARVTR MVQSNRNHPC IIIWSLGNES GGGGNHEALY 

       490        500        510        520        530        540 
HWLKRNDPSR PVQYEGGGAD TTATDIICPM YARVERDQPI PAVPKWGIKK WISLPGEQRP 

       550        560        570        580        590        600 
LILCEYAHAM GNSLGNFADY WQAFREYPRL QGGFIWDWAD QAIRKIFDDG SVGWAYGGDF 

       610        620        630        640        650        660 
GDKPNDRQFC MNGLVFPDRT PHPSLVEAKH AQQYFQFTLL STSPLRVRIT SEYLFRPTDN 

       670        680        690        700        710        720 
EVVRWQVQSA GETLYHGNLT LALPPEGSDE ITLLDSLILP EGARAVWLTL EVTQPRATDW 

       730        740        750        760        770        780 
SEADHRVAWQ QFPLPAPLAL PAPTVPAGAP DLIVSDEVWQ IRAGSQCWTI DRRTGLLSRW 

       790        800        810        820        830        840 
SVGGQEQLLT PLRDQFIRAP LDNDIGVSEV ERIDPNAWVE RWKSAGLYDL EAHCVQCDAQ 

       850        860        870        880        890        900 
RLANETLVDC RWHYLRGEEV VIVSHWRMHF TADGTLRLAV DGERAETLPP LPRVGLHFQV 

       910        920        930        940        950        960 
ADQQAPVSWL GLGPHENYPD RRSSACFARW EQPLAAMTTP YIFPTENGLR CDTQALDWGR 

       970        980        990       1000       1010       1020 
WHVSGHFHFS VQPWSTRQLM ETDHWHKMQA EDGVWITLDG LHMGVGGDDS WTPSVLPQWL 

      1030 
LSQTRWQYEF FLRCL 

« Hide

References

[1]"Complete genome sequence of the N2-fixing broad host range endophyte Klebsiella pneumoniae 342 and virulence predictions verified in mice."
Fouts D.E., Tyler H.L., DeBoy R.T., Daugherty S., Ren Q., Badger J.H., Durkin A.S., Huot H., Shrivastava S., Kothari S., Dodson R.J., Mohamoud Y., Khouri H., Roesch L.F.W., Krogfelt K.A., Struve C., Triplett E.W., Methe B.A.
PLoS Genet. 4:E1000141-E1000141(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 342.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000964 Genomic DNA. Translation: ACI08231.1.
RefSeqYP_002238606.1. NC_011283.1.

3D structure databases

ProteinModelPortalB5XQY2.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING507522.KPK_2776.

Protein family/group databases

CAZyGH2. Glycoside Hydrolase Family 2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACI08231; ACI08231; KPK_2776.
GeneID6934703.
KEGGkpe:KPK_2776.
PATRIC20438958. VBIKlePne121904_2851.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG3250.
HOGENOMHOG000252443.
KOK01190.
OMAVYNENFF.
OrthoDBEOG6XWV0T.
ProtClustDBPRK09525.

Enzyme and pathway databases

BioCycKPNE507522:GI0B-2777-MONOMER.

Family and domain databases

Gene3D2.60.120.260. 1 hit.
2.60.40.320. 2 hits.
2.70.98.10. 1 hit.
3.20.20.80. 1 hit.
HAMAPMF_01687. Beta_gal.
InterProIPR004199. B-gal_small/dom_5.
IPR011013. Gal_mutarotase_SF_dom.
IPR008979. Galactose-bd-like.
IPR014718. Glyco_hydro-type_carb-bd_sub.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR023933. Glyco_hydro_2_beta_Galsidase.
IPR023230. Glyco_hydro_2_CS.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamPF02929. Bgal_small_N. 1 hit.
PF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
PRINTSPR00132. GLHYDRLASE2.
SMARTSM01038. Bgal_small_N. 1 hit.
[Graphical view]
SUPFAMSSF49303. SSF49303. 2 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
SSF74650. SSF74650. 1 hit.
PROSITEPS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameBGAL_KLEP3
AccessionPrimary (citable) accession number: B5XQY2
Entry history
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: November 25, 2008
Last modified: February 19, 2014
This is version 39 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries