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B5XQY2

- BGAL_KLEP3

UniProt

B5XQY2 - BGAL_KLEP3

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Protein

Beta-galactosidase

Gene

lacZ

Organism
Klebsiella pneumoniae (strain 342)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.UniRule annotation

Cofactori

Binds 2 magnesium ions per monomer.UniRule annotation
Binds 1 sodium ion per monomer.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei109 – 1091SubstrateUniRule annotation
Metal bindingi208 – 2081SodiumUniRule annotation
Binding sitei208 – 2081SubstrateUniRule annotation
Sitei365 – 3651Transition state stabilizerUniRule annotation
Sitei399 – 3991Transition state stabilizerUniRule annotation
Metal bindingi424 – 4241Magnesium 1UniRule annotation
Metal bindingi426 – 4261Magnesium 1UniRule annotation
Active sitei469 – 4691Proton donorUniRule annotation
Metal bindingi469 – 4691Magnesium 1UniRule annotation
Binding sitei469 – 4691SubstrateUniRule annotation
Active sitei545 – 5451NucleophileUniRule annotation
Metal bindingi605 – 6051Magnesium 2UniRule annotation
Metal bindingi609 – 6091Sodium; via carbonyl oxygenUniRule annotation
Metal bindingi612 – 6121SodiumUniRule annotation
Binding sitei612 – 6121SubstrateUniRule annotation
Binding sitei1011 – 10111SubstrateUniRule annotation

GO - Molecular functioni

  1. beta-galactosidase activity Source: UniProtKB-EC
  2. carbohydrate binding Source: InterPro
  3. magnesium ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Ligandi

Magnesium, Metal-binding, Sodium

Enzyme and pathway databases

BioCyciKPNE507522:GI0B-2777-MONOMER.

Protein family/group databases

CAZyiGH2. Glycoside Hydrolase Family 2.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-galactosidaseUniRule annotation (EC:3.2.1.23UniRule annotation)
Short name:
Beta-galUniRule annotation
Alternative name(s):
LactaseUniRule annotation
Gene namesi
Name:lacZUniRule annotation
Ordered Locus Names:KPK_2776
OrganismiKlebsiella pneumoniae (strain 342)
Taxonomic identifieri507522 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeKlebsiella
ProteomesiUP000001734: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. beta-galactosidase complex Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10351035Beta-galactosidasePRO_0000367000Add
BLAST

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi507522.KPK_2776.

Structurei

3D structure databases

ProteinModelPortaliB5XQY2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni545 – 5484Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the glycosyl hydrolase 2 family.UniRule annotation

Phylogenomic databases

eggNOGiCOG3250.
HOGENOMiHOG000252443.
KOiK01190.
OMAiQGINKPG.
OrthoDBiEOG6XWV0T.

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
2.60.40.320. 2 hits.
2.70.98.10. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_01687. Beta_gal.
InterProiIPR004199. B-gal_small/dom_5.
IPR011013. Gal_mutarotase_SF_dom.
IPR008979. Galactose-bd-like.
IPR014718. Glyco_hydro-type_carb-bd_sub.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR023933. Glyco_hydro_2_beta_Galsidase.
IPR023230. Glyco_hydro_2_CS.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF02929. Bgal_small_N. 1 hit.
PF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
PRINTSiPR00132. GLHYDRLASE2.
SMARTiSM01038. Bgal_small_N. 1 hit.
[Graphical view]
SUPFAMiSSF49303. SSF49303. 2 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
SSF74650. SSF74650. 1 hit.
PROSITEiPS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B5XQY2-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MQISDTGHSH MPDFHAVLAR EDWQNQTITH LNRLPAHPAF ASWRDELAAR
60 70 80 90 100
DNHLSTRRRQ LDGEWQFVYA RSPFAVDAQW LTQDLPDSRG TPVPSNWQME
110 120 130 140 150
GYDAPIYTNV RYPIDTTPPR VPEDNPTGCY SLHFTVEDTW RENGQTQIIF
160 170 180 190 200
DGVNSAFHLW CNGVWVGYSQ DSRLPAAFDL SPFLRPGDNR LCVMVMRWSA
210 220 230 240 250
GSWLEDQDMW RMSGIFRSVW LLNKPQQRLC DVQLTPALDA LYRDGTLQVQ
260 270 280 290 300
ATVEATEAAL AGLSVGVSLW RGEEQVAAGR QPLGTPAVDE RGHYAERVDF
310 320 330 340 350
ALAVTAPAHW SAETPNCYRA VVTLWRGDEL LEAEAWDIGF RRIEIADGLL
360 370 380 390 400
RLNGKPLLIR GVNRHEHHYL RGQVVTEADM VQDILLMKQN NFNAVRCSHY
410 420 430 440 450
PNAPRWYELC NRYGLYVVDE ANIETHGMVP MNRLSDDPAW LPAFSARVTR
460 470 480 490 500
MVQSNRNHPC IIIWSLGNES GGGGNHEALY HWLKRNDPSR PVQYEGGGAD
510 520 530 540 550
TTATDIICPM YARVERDQPI PAVPKWGIKK WISLPGEQRP LILCEYAHAM
560 570 580 590 600
GNSLGNFADY WQAFREYPRL QGGFIWDWAD QAIRKIFDDG SVGWAYGGDF
610 620 630 640 650
GDKPNDRQFC MNGLVFPDRT PHPSLVEAKH AQQYFQFTLL STSPLRVRIT
660 670 680 690 700
SEYLFRPTDN EVVRWQVQSA GETLYHGNLT LALPPEGSDE ITLLDSLILP
710 720 730 740 750
EGARAVWLTL EVTQPRATDW SEADHRVAWQ QFPLPAPLAL PAPTVPAGAP
760 770 780 790 800
DLIVSDEVWQ IRAGSQCWTI DRRTGLLSRW SVGGQEQLLT PLRDQFIRAP
810 820 830 840 850
LDNDIGVSEV ERIDPNAWVE RWKSAGLYDL EAHCVQCDAQ RLANETLVDC
860 870 880 890 900
RWHYLRGEEV VIVSHWRMHF TADGTLRLAV DGERAETLPP LPRVGLHFQV
910 920 930 940 950
ADQQAPVSWL GLGPHENYPD RRSSACFARW EQPLAAMTTP YIFPTENGLR
960 970 980 990 1000
CDTQALDWGR WHVSGHFHFS VQPWSTRQLM ETDHWHKMQA EDGVWITLDG
1010 1020 1030
LHMGVGGDDS WTPSVLPQWL LSQTRWQYEF FLRCL
Length:1,035
Mass (Da):117,917
Last modified:November 25, 2008 - v1
Checksum:iA44AC3C1E52DFCB5
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000964 Genomic DNA. Translation: ACI08231.1.
RefSeqiWP_012541788.1. NC_011283.1.
YP_002238606.1. NC_011283.1.

Genome annotation databases

EnsemblBacteriaiACI08231; ACI08231; KPK_2776.
GeneIDi6934703.
KEGGikpe:KPK_2776.
PATRICi20438958. VBIKlePne121904_2851.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000964 Genomic DNA. Translation: ACI08231.1 .
RefSeqi WP_012541788.1. NC_011283.1.
YP_002238606.1. NC_011283.1.

3D structure databases

ProteinModelPortali B5XQY2.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 507522.KPK_2776.

Protein family/group databases

CAZyi GH2. Glycoside Hydrolase Family 2.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACI08231 ; ACI08231 ; KPK_2776 .
GeneIDi 6934703.
KEGGi kpe:KPK_2776.
PATRICi 20438958. VBIKlePne121904_2851.

Phylogenomic databases

eggNOGi COG3250.
HOGENOMi HOG000252443.
KOi K01190.
OMAi QGINKPG.
OrthoDBi EOG6XWV0T.

Enzyme and pathway databases

BioCyci KPNE507522:GI0B-2777-MONOMER.

Family and domain databases

Gene3Di 2.60.120.260. 1 hit.
2.60.40.320. 2 hits.
2.70.98.10. 1 hit.
3.20.20.80. 1 hit.
HAMAPi MF_01687. Beta_gal.
InterProi IPR004199. B-gal_small/dom_5.
IPR011013. Gal_mutarotase_SF_dom.
IPR008979. Galactose-bd-like.
IPR014718. Glyco_hydro-type_carb-bd_sub.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR023933. Glyco_hydro_2_beta_Galsidase.
IPR023230. Glyco_hydro_2_CS.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
Pfami PF02929. Bgal_small_N. 1 hit.
PF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view ]
PRINTSi PR00132. GLHYDRLASE2.
SMARTi SM01038. Bgal_small_N. 1 hit.
[Graphical view ]
SUPFAMi SSF49303. SSF49303. 2 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
SSF74650. SSF74650. 1 hit.
PROSITEi PS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Complete genome sequence of the N2-fixing broad host range endophyte Klebsiella pneumoniae 342 and virulence predictions verified in mice."
    Fouts D.E., Tyler H.L., DeBoy R.T., Daugherty S., Ren Q., Badger J.H., Durkin A.S., Huot H., Shrivastava S., Kothari S., Dodson R.J., Mohamoud Y., Khouri H., Roesch L.F.W., Krogfelt K.A., Struve C., Triplett E.W., Methe B.A.
    PLoS Genet. 4:E1000141-E1000141(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 342.

Entry informationi

Entry nameiBGAL_KLEP3
AccessioniPrimary (citable) accession number: B5XQY2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: November 25, 2008
Last modified: October 29, 2014
This is version 42 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3