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B5XQY2

- BGAL_KLEP3

UniProt

B5XQY2 - BGAL_KLEP3

Protein

Beta-galactosidase

Gene

lacZ

Organism
Klebsiella pneumoniae (strain 342)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 41 (01 Oct 2014)
      Sequence version 1 (25 Nov 2008)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.UniRule annotation

    Cofactori

    Binds 2 magnesium ions per monomer.UniRule annotation
    Binds 1 sodium ion per monomer.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei109 – 1091SubstrateUniRule annotation
    Metal bindingi208 – 2081SodiumUniRule annotation
    Binding sitei208 – 2081SubstrateUniRule annotation
    Sitei365 – 3651Transition state stabilizerUniRule annotation
    Sitei399 – 3991Transition state stabilizerUniRule annotation
    Metal bindingi424 – 4241Magnesium 1UniRule annotation
    Metal bindingi426 – 4261Magnesium 1UniRule annotation
    Active sitei469 – 4691Proton donorUniRule annotation
    Metal bindingi469 – 4691Magnesium 1UniRule annotation
    Binding sitei469 – 4691SubstrateUniRule annotation
    Active sitei545 – 5451NucleophileUniRule annotation
    Metal bindingi605 – 6051Magnesium 2UniRule annotation
    Metal bindingi609 – 6091Sodium; via carbonyl oxygenUniRule annotation
    Metal bindingi612 – 6121SodiumUniRule annotation
    Binding sitei612 – 6121SubstrateUniRule annotation
    Binding sitei1011 – 10111SubstrateUniRule annotation

    GO - Molecular functioni

    1. beta-galactosidase activity Source: UniProtKB-EC
    2. carbohydrate binding Source: InterPro
    3. magnesium ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. carbohydrate metabolic process Source: InterPro

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Ligandi

    Magnesium, Metal-binding, Sodium

    Enzyme and pathway databases

    BioCyciKPNE507522:GI0B-2777-MONOMER.

    Protein family/group databases

    CAZyiGH2. Glycoside Hydrolase Family 2.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-galactosidaseUniRule annotation (EC:3.2.1.23UniRule annotation)
    Short name:
    Beta-galUniRule annotation
    Alternative name(s):
    LactaseUniRule annotation
    Gene namesi
    Name:lacZUniRule annotation
    Ordered Locus Names:KPK_2776
    OrganismiKlebsiella pneumoniae (strain 342)
    Taxonomic identifieri507522 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeKlebsiella
    ProteomesiUP000001734: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    1. beta-galactosidase complex Source: InterPro

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10351035Beta-galactosidasePRO_0000367000Add
    BLAST

    Interactioni

    Subunit structurei

    Homotetramer.UniRule annotation

    Protein-protein interaction databases

    STRINGi507522.KPK_2776.

    Structurei

    3D structure databases

    ProteinModelPortaliB5XQY2.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni545 – 5484Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 2 family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG3250.
    HOGENOMiHOG000252443.
    KOiK01190.
    OMAiQGINKPG.
    OrthoDBiEOG6XWV0T.

    Family and domain databases

    Gene3Di2.60.120.260. 1 hit.
    2.60.40.320. 2 hits.
    2.70.98.10. 1 hit.
    3.20.20.80. 1 hit.
    HAMAPiMF_01687. Beta_gal.
    InterProiIPR004199. B-gal_small/dom_5.
    IPR011013. Gal_mutarotase_SF_dom.
    IPR008979. Galactose-bd-like.
    IPR014718. Glyco_hydro-type_carb-bd_sub.
    IPR006101. Glyco_hydro_2.
    IPR013812. Glyco_hydro_2/20_Ig-like.
    IPR023232. Glyco_hydro_2_AS.
    IPR023933. Glyco_hydro_2_beta_Galsidase.
    IPR023230. Glyco_hydro_2_CS.
    IPR006102. Glyco_hydro_2_Ig-like.
    IPR006104. Glyco_hydro_2_N.
    IPR006103. Glyco_hydro_2_TIM.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PfamiPF02929. Bgal_small_N. 1 hit.
    PF00703. Glyco_hydro_2. 1 hit.
    PF02836. Glyco_hydro_2_C. 1 hit.
    PF02837. Glyco_hydro_2_N. 1 hit.
    [Graphical view]
    PRINTSiPR00132. GLHYDRLASE2.
    SMARTiSM01038. Bgal_small_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF49303. SSF49303. 2 hits.
    SSF49785. SSF49785. 1 hit.
    SSF51445. SSF51445. 1 hit.
    SSF74650. SSF74650. 1 hit.
    PROSITEiPS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
    PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    B5XQY2-1 [UniParc]FASTAAdd to Basket

    « Hide

    MQISDTGHSH MPDFHAVLAR EDWQNQTITH LNRLPAHPAF ASWRDELAAR     50
    DNHLSTRRRQ LDGEWQFVYA RSPFAVDAQW LTQDLPDSRG TPVPSNWQME 100
    GYDAPIYTNV RYPIDTTPPR VPEDNPTGCY SLHFTVEDTW RENGQTQIIF 150
    DGVNSAFHLW CNGVWVGYSQ DSRLPAAFDL SPFLRPGDNR LCVMVMRWSA 200
    GSWLEDQDMW RMSGIFRSVW LLNKPQQRLC DVQLTPALDA LYRDGTLQVQ 250
    ATVEATEAAL AGLSVGVSLW RGEEQVAAGR QPLGTPAVDE RGHYAERVDF 300
    ALAVTAPAHW SAETPNCYRA VVTLWRGDEL LEAEAWDIGF RRIEIADGLL 350
    RLNGKPLLIR GVNRHEHHYL RGQVVTEADM VQDILLMKQN NFNAVRCSHY 400
    PNAPRWYELC NRYGLYVVDE ANIETHGMVP MNRLSDDPAW LPAFSARVTR 450
    MVQSNRNHPC IIIWSLGNES GGGGNHEALY HWLKRNDPSR PVQYEGGGAD 500
    TTATDIICPM YARVERDQPI PAVPKWGIKK WISLPGEQRP LILCEYAHAM 550
    GNSLGNFADY WQAFREYPRL QGGFIWDWAD QAIRKIFDDG SVGWAYGGDF 600
    GDKPNDRQFC MNGLVFPDRT PHPSLVEAKH AQQYFQFTLL STSPLRVRIT 650
    SEYLFRPTDN EVVRWQVQSA GETLYHGNLT LALPPEGSDE ITLLDSLILP 700
    EGARAVWLTL EVTQPRATDW SEADHRVAWQ QFPLPAPLAL PAPTVPAGAP 750
    DLIVSDEVWQ IRAGSQCWTI DRRTGLLSRW SVGGQEQLLT PLRDQFIRAP 800
    LDNDIGVSEV ERIDPNAWVE RWKSAGLYDL EAHCVQCDAQ RLANETLVDC 850
    RWHYLRGEEV VIVSHWRMHF TADGTLRLAV DGERAETLPP LPRVGLHFQV 900
    ADQQAPVSWL GLGPHENYPD RRSSACFARW EQPLAAMTTP YIFPTENGLR 950
    CDTQALDWGR WHVSGHFHFS VQPWSTRQLM ETDHWHKMQA EDGVWITLDG 1000
    LHMGVGGDDS WTPSVLPQWL LSQTRWQYEF FLRCL 1035
    Length:1,035
    Mass (Da):117,917
    Last modified:November 25, 2008 - v1
    Checksum:iA44AC3C1E52DFCB5
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000964 Genomic DNA. Translation: ACI08231.1.
    RefSeqiYP_002238606.1. NC_011283.1.

    Genome annotation databases

    EnsemblBacteriaiACI08231; ACI08231; KPK_2776.
    GeneIDi6934703.
    KEGGikpe:KPK_2776.
    PATRICi20438958. VBIKlePne121904_2851.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000964 Genomic DNA. Translation: ACI08231.1 .
    RefSeqi YP_002238606.1. NC_011283.1.

    3D structure databases

    ProteinModelPortali B5XQY2.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 507522.KPK_2776.

    Protein family/group databases

    CAZyi GH2. Glycoside Hydrolase Family 2.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ACI08231 ; ACI08231 ; KPK_2776 .
    GeneIDi 6934703.
    KEGGi kpe:KPK_2776.
    PATRICi 20438958. VBIKlePne121904_2851.

    Phylogenomic databases

    eggNOGi COG3250.
    HOGENOMi HOG000252443.
    KOi K01190.
    OMAi QGINKPG.
    OrthoDBi EOG6XWV0T.

    Enzyme and pathway databases

    BioCyci KPNE507522:GI0B-2777-MONOMER.

    Family and domain databases

    Gene3Di 2.60.120.260. 1 hit.
    2.60.40.320. 2 hits.
    2.70.98.10. 1 hit.
    3.20.20.80. 1 hit.
    HAMAPi MF_01687. Beta_gal.
    InterProi IPR004199. B-gal_small/dom_5.
    IPR011013. Gal_mutarotase_SF_dom.
    IPR008979. Galactose-bd-like.
    IPR014718. Glyco_hydro-type_carb-bd_sub.
    IPR006101. Glyco_hydro_2.
    IPR013812. Glyco_hydro_2/20_Ig-like.
    IPR023232. Glyco_hydro_2_AS.
    IPR023933. Glyco_hydro_2_beta_Galsidase.
    IPR023230. Glyco_hydro_2_CS.
    IPR006102. Glyco_hydro_2_Ig-like.
    IPR006104. Glyco_hydro_2_N.
    IPR006103. Glyco_hydro_2_TIM.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    Pfami PF02929. Bgal_small_N. 1 hit.
    PF00703. Glyco_hydro_2. 1 hit.
    PF02836. Glyco_hydro_2_C. 1 hit.
    PF02837. Glyco_hydro_2_N. 1 hit.
    [Graphical view ]
    PRINTSi PR00132. GLHYDRLASE2.
    SMARTi SM01038. Bgal_small_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49303. SSF49303. 2 hits.
    SSF49785. SSF49785. 1 hit.
    SSF51445. SSF51445. 1 hit.
    SSF74650. SSF74650. 1 hit.
    PROSITEi PS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
    PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complete genome sequence of the N2-fixing broad host range endophyte Klebsiella pneumoniae 342 and virulence predictions verified in mice."
      Fouts D.E., Tyler H.L., DeBoy R.T., Daugherty S., Ren Q., Badger J.H., Durkin A.S., Huot H., Shrivastava S., Kothari S., Dodson R.J., Mohamoud Y., Khouri H., Roesch L.F.W., Krogfelt K.A., Struve C., Triplett E.W., Methe B.A.
      PLoS Genet. 4:E1000141-E1000141(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 342.

    Entry informationi

    Entry nameiBGAL_KLEP3
    AccessioniPrimary (citable) accession number: B5XQY2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 24, 2009
    Last sequence update: November 25, 2008
    Last modified: October 1, 2014
    This is version 41 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3