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B5XQJ0

- MHPB_KLEP3

UniProt

B5XQJ0 - MHPB_KLEP3

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Protein

2,3-dihydroxyphenylpropionate/2,3-dihydroxicinnamic acid 1,2-dioxygenase

Gene

mhpB

Organism
Klebsiella pneumoniae (strain 342)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the non-heme iron(II)-dependent oxidative cleavage of 2,3-dihydroxyphenylpropionic acid and 2,3-dihydroxicinnamic acid into 2-hydroxy-6-ketononadienedioate and 2-hydroxy-6-ketononatrienedioate, respectively.UniRule annotation

Catalytic activityi

3-(2,3-dihydroxyphenyl)propanoate + O2 = (2Z,4E)-2-hydroxy-6-oxonona-2,4-diene-1,9-dioate.UniRule annotation
(2E)-3-(2,3-dihydroxyphenyl)prop-2-enoate + O2 = (2Z,4E,7E)-2-hydroxy-6-oxonona-2,4,7-triene-1,9-dioate.UniRule annotation

Cofactori

Fe2+ ion.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei115 – 1151Proton donorUniRule annotation
Active sitei179 – 1791Proton acceptorUniRule annotation

GO - Molecular functioni

  1. 3-carboxyethylcatechol 2,3-dioxygenase activity Source: UniProtKB-HAMAP
  2. ferrous iron binding Source: InterPro

GO - Biological processi

  1. 3-phenylpropionate catabolic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Dioxygenase, Oxidoreductase

Keywords - Biological processi

Aromatic hydrocarbons catabolism

Keywords - Ligandi

Iron

Enzyme and pathway databases

BioCyciKPNE507522:GI0B-2203-MONOMER.
UniPathwayiUPA00714.

Names & Taxonomyi

Protein namesi
Recommended name:
2,3-dihydroxyphenylpropionate/2,3-dihydroxicinnamic acid 1,2-dioxygenaseUniRule annotation (EC:1.13.11.16UniRule annotation)
Alternative name(s):
3-carboxyethylcatechol 2,3-dioxygenaseUniRule annotation
Gene namesi
Name:mhpBUniRule annotation
Ordered Locus Names:KPK_2202
OrganismiKlebsiella pneumoniae (strain 342)
Taxonomic identifieri507522 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeKlebsiella
ProteomesiUP000001734: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 3143142,3-dihydroxyphenylpropionate/2,3-dihydroxicinnamic acid 1,2-dioxygenasePRO_1000187009Add
BLAST

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi507522.KPK_2202.

Family & Domainsi

Sequence similaritiesi

Belongs to the LigB/MhpB extradiol dioxygenase family.UniRule annotation

Phylogenomic databases

eggNOGiNOG08474.
HOGENOMiHOG000069851.
KOiK05713.
OMAiHCLSHTP.
OrthoDBiEOG62ZHSQ.

Family and domain databases

Gene3Di3.40.830.10. 2 hits.
HAMAPiMF_01653. MhpB.
InterProiIPR023789. DHPP/DHXA_dioxygenase.
IPR004183. Xdiol_dOase_suB.
[Graphical view]
PfamiPF02900. LigB. 1 hit.
[Graphical view]
SUPFAMiSSF53213. SSF53213. 2 hits.

Sequencei

Sequence statusi: Complete.

B5XQJ0-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDAYLHCLSH TPLVGFVDPE QAVLDEVNGV IADARRRISA FEPELVVLFA
60 70 80 90 100
PDHYNGFFYD VMPPFCLGVG ATAIGDFASA AGDLPVPTEL AEACAHAVIN
110 120 130 140 150
SGIDLAVSYN MQVDHGFAQP LEFLLGGLDR VPVLPVFING VAAPLPGFQR
160 170 180 190 200
TRLLGEAMGR FLNTLNKRVL ILGSGGLSHQ PPVPELAKAD AHLRDRLLGS
210 220 230 240 250
GKQLPPDERE RRQQRVINAA RRFTEDQRSL HPLNPVWDNR FMSLLEQGRL
260 270 280 290 300
AELDAIGNDE LSAMAGKSTH EIKTWVAAFA ALSAFGCWRS EGRYYRPIPE
310
WIAGFGSLSA TTEI
Length:314
Mass (Da):34,295
Last modified:November 25, 2008 - v1
Checksum:iA961455CEE055FD4
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000964 Genomic DNA. Translation: ACI06844.1.
RefSeqiWP_012541409.1. NC_011283.1.
YP_002238038.1. NC_011283.1.

Genome annotation databases

EnsemblBacteriaiACI06844; ACI06844; KPK_2202.
GeneIDi6936698.
KEGGikpe:KPK_2202.
PATRICi20437860. VBIKlePne121904_2306.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000964 Genomic DNA. Translation: ACI06844.1 .
RefSeqi WP_012541409.1. NC_011283.1.
YP_002238038.1. NC_011283.1.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 507522.KPK_2202.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACI06844 ; ACI06844 ; KPK_2202 .
GeneIDi 6936698.
KEGGi kpe:KPK_2202.
PATRICi 20437860. VBIKlePne121904_2306.

Phylogenomic databases

eggNOGi NOG08474.
HOGENOMi HOG000069851.
KOi K05713.
OMAi HCLSHTP.
OrthoDBi EOG62ZHSQ.

Enzyme and pathway databases

UniPathwayi UPA00714 .
BioCyci KPNE507522:GI0B-2203-MONOMER.

Family and domain databases

Gene3Di 3.40.830.10. 2 hits.
HAMAPi MF_01653. MhpB.
InterProi IPR023789. DHPP/DHXA_dioxygenase.
IPR004183. Xdiol_dOase_suB.
[Graphical view ]
Pfami PF02900. LigB. 1 hit.
[Graphical view ]
SUPFAMi SSF53213. SSF53213. 2 hits.
ProtoNeti Search...

Publicationsi

  1. "Complete genome sequence of the N2-fixing broad host range endophyte Klebsiella pneumoniae 342 and virulence predictions verified in mice."
    Fouts D.E., Tyler H.L., DeBoy R.T., Daugherty S., Ren Q., Badger J.H., Durkin A.S., Huot H., Shrivastava S., Kothari S., Dodson R.J., Mohamoud Y., Khouri H., Roesch L.F.W., Krogfelt K.A., Struve C., Triplett E.W., Methe B.A.
    PLoS Genet. 4:E1000141-E1000141(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 342.

Entry informationi

Entry nameiMHPB_KLEP3
AccessioniPrimary (citable) accession number: B5XQJ0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: November 25, 2008
Last modified: October 1, 2014
This is version 38 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3