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B5XQJ0

- MHPB_KLEP3

UniProt

B5XQJ0 - MHPB_KLEP3

Protein

2,3-dihydroxyphenylpropionate/2,3-dihydroxicinnamic acid 1,2-dioxygenase

Gene

mhpB

Organism
Klebsiella pneumoniae (strain 342)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 38 (01 Oct 2014)
      Sequence version 1 (25 Nov 2008)
      Previous versions | rss
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    Functioni

    Catalyzes the non-heme iron(II)-dependent oxidative cleavage of 2,3-dihydroxyphenylpropionic acid and 2,3-dihydroxicinnamic acid into 2-hydroxy-6-ketononadienedioate and 2-hydroxy-6-ketononatrienedioate, respectively.UniRule annotation

    Catalytic activityi

    3-(2,3-dihydroxyphenyl)propanoate + O2 = (2Z,4E)-2-hydroxy-6-oxonona-2,4-diene-1,9-dioate.UniRule annotation
    (2E)-3-(2,3-dihydroxyphenyl)prop-2-enoate + O2 = (2Z,4E,7E)-2-hydroxy-6-oxonona-2,4,7-triene-1,9-dioate.UniRule annotation

    Cofactori

    Fe2+ ion.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei115 – 1151Proton donorUniRule annotation
    Active sitei179 – 1791Proton acceptorUniRule annotation

    GO - Molecular functioni

    1. 3-carboxyethylcatechol 2,3-dioxygenase activity Source: UniProtKB-HAMAP
    2. ferrous iron binding Source: InterPro

    GO - Biological processi

    1. 3-phenylpropionate catabolic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Dioxygenase, Oxidoreductase

    Keywords - Biological processi

    Aromatic hydrocarbons catabolism

    Keywords - Ligandi

    Iron

    Enzyme and pathway databases

    BioCyciKPNE507522:GI0B-2203-MONOMER.
    UniPathwayiUPA00714.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    2,3-dihydroxyphenylpropionate/2,3-dihydroxicinnamic acid 1,2-dioxygenaseUniRule annotation (EC:1.13.11.16UniRule annotation)
    Alternative name(s):
    3-carboxyethylcatechol 2,3-dioxygenaseUniRule annotation
    Gene namesi
    Name:mhpBUniRule annotation
    Ordered Locus Names:KPK_2202
    OrganismiKlebsiella pneumoniae (strain 342)
    Taxonomic identifieri507522 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeKlebsiella
    ProteomesiUP000001734: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 3143142,3-dihydroxyphenylpropionate/2,3-dihydroxicinnamic acid 1,2-dioxygenasePRO_1000187009Add
    BLAST

    Interactioni

    Subunit structurei

    Homotetramer.UniRule annotation

    Protein-protein interaction databases

    STRINGi507522.KPK_2202.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the LigB/MhpB extradiol dioxygenase family.UniRule annotation

    Phylogenomic databases

    eggNOGiNOG08474.
    HOGENOMiHOG000069851.
    KOiK05713.
    OMAiHCLSHTP.
    OrthoDBiEOG62ZHSQ.

    Family and domain databases

    Gene3Di3.40.830.10. 2 hits.
    HAMAPiMF_01653. MhpB.
    InterProiIPR023789. DHPP/DHXA_dioxygenase.
    IPR004183. Xdiol_dOase_suB.
    [Graphical view]
    PfamiPF02900. LigB. 1 hit.
    [Graphical view]
    SUPFAMiSSF53213. SSF53213. 2 hits.

    Sequencei

    Sequence statusi: Complete.

    B5XQJ0-1 [UniParc]FASTAAdd to Basket

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    MDAYLHCLSH TPLVGFVDPE QAVLDEVNGV IADARRRISA FEPELVVLFA    50
    PDHYNGFFYD VMPPFCLGVG ATAIGDFASA AGDLPVPTEL AEACAHAVIN 100
    SGIDLAVSYN MQVDHGFAQP LEFLLGGLDR VPVLPVFING VAAPLPGFQR 150
    TRLLGEAMGR FLNTLNKRVL ILGSGGLSHQ PPVPELAKAD AHLRDRLLGS 200
    GKQLPPDERE RRQQRVINAA RRFTEDQRSL HPLNPVWDNR FMSLLEQGRL 250
    AELDAIGNDE LSAMAGKSTH EIKTWVAAFA ALSAFGCWRS EGRYYRPIPE 300
    WIAGFGSLSA TTEI 314
    Length:314
    Mass (Da):34,295
    Last modified:November 25, 2008 - v1
    Checksum:iA961455CEE055FD4
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000964 Genomic DNA. Translation: ACI06844.1.
    RefSeqiWP_012541409.1. NC_011283.1.
    YP_002238038.1. NC_011283.1.

    Genome annotation databases

    EnsemblBacteriaiACI06844; ACI06844; KPK_2202.
    GeneIDi6936698.
    KEGGikpe:KPK_2202.
    PATRICi20437860. VBIKlePne121904_2306.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000964 Genomic DNA. Translation: ACI06844.1 .
    RefSeqi WP_012541409.1. NC_011283.1.
    YP_002238038.1. NC_011283.1.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 507522.KPK_2202.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ACI06844 ; ACI06844 ; KPK_2202 .
    GeneIDi 6936698.
    KEGGi kpe:KPK_2202.
    PATRICi 20437860. VBIKlePne121904_2306.

    Phylogenomic databases

    eggNOGi NOG08474.
    HOGENOMi HOG000069851.
    KOi K05713.
    OMAi HCLSHTP.
    OrthoDBi EOG62ZHSQ.

    Enzyme and pathway databases

    UniPathwayi UPA00714 .
    BioCyci KPNE507522:GI0B-2203-MONOMER.

    Family and domain databases

    Gene3Di 3.40.830.10. 2 hits.
    HAMAPi MF_01653. MhpB.
    InterProi IPR023789. DHPP/DHXA_dioxygenase.
    IPR004183. Xdiol_dOase_suB.
    [Graphical view ]
    Pfami PF02900. LigB. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53213. SSF53213. 2 hits.
    ProtoNeti Search...

    Publicationsi

    1. "Complete genome sequence of the N2-fixing broad host range endophyte Klebsiella pneumoniae 342 and virulence predictions verified in mice."
      Fouts D.E., Tyler H.L., DeBoy R.T., Daugherty S., Ren Q., Badger J.H., Durkin A.S., Huot H., Shrivastava S., Kothari S., Dodson R.J., Mohamoud Y., Khouri H., Roesch L.F.W., Krogfelt K.A., Struve C., Triplett E.W., Methe B.A.
      PLoS Genet. 4:E1000141-E1000141(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 342.

    Entry informationi

    Entry nameiMHPB_KLEP3
    AccessioniPrimary (citable) accession number: B5XQJ0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 28, 2009
    Last sequence update: November 25, 2008
    Last modified: October 1, 2014
    This is version 38 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3