Reviewed, UniProtKB/Swiss-Prot B5XQ81 (DADA_KLEP3)

Names and origin

Protein names

Recommended name:
    D-amino acid dehydrogenase small subunit
    EC=1.4.99.1

Gene names

Name:	dadA
Ordered Locus Names:	KPK_1985

Organism

Klebsiella pneumoniae (strain 342) [Complete proteome] [HAMAP]

Taxonomic identifier

507522 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Klebsiella

Protein attributes

Sequence length	432 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Oxidative deamination of D-amino acids By similarity.
Catalytic activity	A D-amino acid + H2O + acceptor = a 2-oxo acid + NH3 + reduced acceptor. HAMAP MF_01202
Cofactor	FAD By similarity.
Pathway	Amino-acid degradation; D-alanine degradation; NH(3) and pyruvate from D-alanine: step 1/1. HAMAP MF_01202
Subunit structure	Heterodimer of a small and a large subunit By similarity.
Sequence similarities	Belongs to the dadA oxidoreductase family.

Ontologies

Keywords
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	alanine catabolic process Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	D-amino-acid dehydrogenase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 432	432	D-amino acid dehydrogenase small subunit HAMAP MF_01202		PRO_1000138656
Regions
	Nucleotide binding	3 – 17	15	FAD Potential

Sequences

	Sequence		Length	Mass (Da)
	B5XQ81-1 [UniParc]. Last modified November 25, 2008. Version 1. Checksum: B435F1BF156C218C	FASTA	432	47,206
	10 20 30 40 50 60 MRVVILGSGV VGVASAWYLS QAGHEVTVID RQPGPAEETS AANAGQISPG YAAPWAAPGV 70 80 90 100 110 120 PLKAIKWMFQ RHAPLAIGLD GTSFQLKWMW QMLRNCDTRH YMENKGRMVR LAEYSRDCLK 130 140 150 160 170 180 ALRDTTGIQY EGRQGGTLQL FRTAKQYENA TRDIAVLEDA GVPYQLLEAK RLAEVEPALA 190 200 210 220 230 240 EVSHKLTGGL RLPNDETGDC QLFTTRLAAM AEQAGVTFRF NTAVDALLQE GDRIAGVKCG 250 260 270 280 290 300 DEIIKGDAYV MAFGSYSTAM LKGLVDIPVY PLKGYSLTIP IAQEDGAPVS TILDETYKIA 310 320 330 340 350 360 ITRFDQRIRV GGMAEIVGFN KALLQPRRET LEMVVRDLFP RGGHVEQATF WTGLRPMTPD 370 380 390 400 410 420 GTPVVGRTAY KNLWLNTGHG TLGWTMACGS GQLISDLISG RTPAIPYDDL AVARYSPGFT 430 PARPQHLHGA HN

References

[1]

"Complete genome sequence of the N2-fixing broad host range endophyte Klebsiella pneumoniae 342 and virulence predictions verified in mice." Fouts D.E., Tyler H.L., DeBoy R.T., Daugherty S., Ren Q., Badger J.H., Durkin A.S., Huot H., Shrivastava S., Kothari S., Dodson R.J., Mohamoud Y., Khouri H., Roesch L.F.W., Krogfelt K.A., Struve C., Triplett E.W., Methe B.A. PLoS Genet. 4:E1000141-E1000141(2008) [PubMed: 18654632] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000964 Genomic DNA. Translation: ACI11038.1.
RefSeq	YP_002237824.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	6937580.
GenomeReviews	Gene locus KPK_1985 in contig CP000964_GR.
KEGG	kpe:KPK_1985.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	MFQKHAP.
Family and domain databases
HAMAP	MF_01202. [Tree]
InterPro	IPR006076. FAD-dep_OxRdtase. [Graphical view]
Pfam	PF01266. DAO. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DADA_KLEP3

Accession

Primary (citable) accession number: B5XQ81

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	November 25, 2008
Last modified:	November 3, 2009
This is version 10 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)