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Protein

Ribosomal RNA small subunit methyltransferase F

Gene

rsmF

Organism
Klebsiella pneumoniae (strain 342)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Specifically methylates the cytosine at position 1407 (m5C1407) of 16S rRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + cytosine(1407) in 16S rRNA = S-adenosyl-L-homocysteine + 5-methylcytosine(1407) in 16S rRNA.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei149S-adenosyl-L-methionineUniRule annotation1
Binding sitei176S-adenosyl-L-methionineUniRule annotation1
Binding sitei194S-adenosyl-L-methionineUniRule annotation1
Active sitei247NucleophileUniRule annotation1

GO - Molecular functioni

Keywordsi

Molecular functionMethyltransferase, RNA-binding, Transferase
Biological processrRNA processing
LigandS-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
Ribosomal RNA small subunit methyltransferase FUniRule annotation (EC:2.1.1.178UniRule annotation)
Alternative name(s):
16S rRNA m5C1407 methyltransferaseUniRule annotation
rRNA (cytosine-C(5)-)-methyltransferase RsmFUniRule annotation
Gene namesi
Name:rsmFUniRule annotation
Ordered Locus Names:KPK_1939
OrganismiKlebsiella pneumoniae (strain 342)
Taxonomic identifieri507522 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeKlebsiella
Proteomesi
  • UP000001734 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10001475711 – 477Ribosomal RNA small subunit methyltransferase FAdd BLAST477

Structurei

3D structure databases

ProteinModelPortaliB5XQ35.
SMRiB5XQ35.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni125 – 131S-adenosyl-L-methionine bindingUniRule annotation7

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. RsmB/NOP family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000218115.
KOiK11392.
OMAiDPLFHAG.

Family and domain databases

HAMAPiMF_01579. 16SrRNA_methyltr_F. 1 hit.
InterProiView protein in InterPro
IPR018314. Fmu/NOL1/Nop2p_CS.
IPR031341. Methyltr_RsmF_N.
IPR001678. MeTrfase_RsmB/NOP2.
IPR027391. Nol1_Nop2_Fmu_2.
IPR011023. Nop2p.
IPR023267. RCMT.
IPR023545. rRNA_ssu_MeTfrase_F.
IPR029063. SAM-dependent_MTases.
PfamiView protein in Pfam
PF01189. Methyltr_RsmB-F. 1 hit.
PF17125. Methyltr_RsmF_N. 1 hit.
PF13636. Methyltranf_PUA. 1 hit.
PRINTSiPR02008. RCMTFAMILY.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00446. nop2p. 1 hit.
PROSITEiView protein in PROSITE
PS01153. NOL1_NOP2_SUN. 1 hit.
PS51686. SAM_MT_RSMB_NOP. 1 hit.

Sequencei

Sequence statusi: Complete.

B5XQ35-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAENTVYFPE AFLAQMRAAM PAHLSFDDFI AACQRPLRRS IRVNTLKISV
60 70 80 90 100
ADFLSLVAPY GWQLTPVPWC EEGFWIERDG DDALPLGSTA EHLSGLFYIQ
110 120 130 140 150
EASSMLPVAA LFADNPQPER VMDVAAAPGS KTTQIAARMG NAGGILANEF
160 170 180 190 200
SASRVKVLHA NISRCGISNV ALTHFDGRVF GAALPEAFDA ILLDAPCSGE
210 220 230 240 250
GVVRKDADAL KNWSPESNLD IAATQRELID SAFHALRPGG TLVYSTCTLN
260 270 280 290 300
REENQSVVQW LLSRYPQAVE ILPLGDLFSG AADALTAEGF LHVFPQIYDC
310 320 330 340 350
EGFFVARLRK TAAIDPLPAP GYKVGKFPFT PLKSREAAAV TAAANAVGLA
360 370 380 390 400
WDAGHTLWQR DKELWLFPQA MEPLFGQVRF SRIGVRLAEV HNKGYRWQHE
410 420 430 440 450
AVIAFAAPQR AFELTQEEAE EWYRGRDVYP QTAPQQDEAI VTFQGVPLGL
460 470
AKRVGSRLKN SYPRELVRDG KLFAGKV
Length:477
Mass (Da):52,392
Last modified:November 25, 2008 - v1
Checksum:iC65B0A7B9D42D7A0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000964 Genomic DNA. Translation: ACI10246.1.
RefSeqiWP_012541265.1. NC_011283.1.

Genome annotation databases

EnsemblBacteriaiACI10246; ACI10246; KPK_1939.
KEGGikpe:KPK_1939.

Similar proteinsi

Entry informationi

Entry nameiRSMF_KLEP3
AccessioniPrimary (citable) accession number: B5XQ35
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: November 25, 2008
Last modified: June 7, 2017
This is version 59 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome