ID   B5XPW1_KLEP3            Unreviewed;       495 AA.
AC   B5XPW1;
DT   25-NOV-2008, integrated into UniProtKB/TrEMBL.
DT   25-NOV-2008, sequence version 1.
DT   27-MAR-2024, entry version 75.
DE   SubName: Full=Alpha-amylase, cytoplasmic {ECO:0000313|EMBL:ACI10366.1};
DE            EC=3.2.1.1 {ECO:0000313|EMBL:ACI10366.1};
GN   Name=amyA {ECO:0000313|EMBL:ACI10366.1};
GN   OrderedLocusNames=KPK_1862 {ECO:0000313|EMBL:ACI10366.1};
OS   Klebsiella pneumoniae (strain 342).
OC   Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
OX   NCBI_TaxID=507522 {ECO:0000313|EMBL:ACI10366.1, ECO:0000313|Proteomes:UP000001734};
RN   [1] {ECO:0000313|EMBL:ACI10366.1, ECO:0000313|Proteomes:UP000001734}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=342 {ECO:0000313|EMBL:ACI10366.1,
RC   ECO:0000313|Proteomes:UP000001734};
RX   PubMed=18654632; DOI=10.1371/journal.pgen.1000141;
RA   Fouts D.E., Tyler H.L., DeBoy R.T., Daugherty S., Ren Q., Badger J.H.,
RA   Durkin A.S., Huot H., Shrivastava S., Kothari S., Dodson R.J., Mohamoud Y.,
RA   Khouri H., Roesch L.F., Krogfelt K.A., Struve C., Triplett E.W.,
RA   Methe B.A.;
RT   "Complete genome sequence of the N2-fixing broad host range endophyte
RT   Klebsiella pneumoniae 342 and virulence predictions verified in mice.";
RL   PLoS Genet. 4:E1000141-E1000141(2008).
CC   -!- COFACTOR:
CC       Name=Ca(2+); Xref=ChEBI:CHEBI:29108;
CC         Evidence={ECO:0000256|ARBA:ARBA00001913};
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DR   EMBL; CP000964; ACI10366.1; -; Genomic_DNA.
DR   AlphaFoldDB; B5XPW1; -.
DR   CAZy; GH13; Glycoside Hydrolase Family 13.
DR   KEGG; kpe:KPK_1862; -.
DR   HOGENOM; CLU_024572_2_0_6; -.
DR   Proteomes; UP000001734; Chromosome.
DR   GO; GO:0004556; F:alpha-amylase activity; IEA:UniProtKB-EC.
DR   GO; GO:0005509; F:calcium ion binding; IEA:InterPro.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR   CDD; cd11318; AmyAc_bac_fung_AmyA; 1.
DR   Gene3D; 2.40.30.140; -; 1.
DR   Gene3D; 3.20.20.80; Glycosidases; 1.
DR   Gene3D; 2.60.40.1180; Golgi alpha-mannosidase II; 1.
DR   InterPro; IPR013776; A-amylase_thermo.
DR   InterPro; IPR006047; Glyco_hydro_13_cat_dom.
DR   InterPro; IPR013780; Glyco_hydro_b.
DR   InterPro; IPR017853; Glycoside_hydrolase_SF.
DR   PANTHER; PTHR43447; ALPHA-AMYLASE; 1.
DR   PANTHER; PTHR43447:SF49; ALPHA-AMYLASE; 1.
DR   Pfam; PF00128; Alpha-amylase; 1.
DR   PIRSF; PIRSF001021; Alph-amls_thrmst; 1.
DR   SMART; SM00642; Aamy; 1.
DR   SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR   SUPFAM; SSF51011; Glycosyl hydrolase domain; 1.
PE   4: Predicted;
KW   Calcium {ECO:0000256|PIRSR:PIRSR001021-2};
KW   Glycosidase {ECO:0000313|EMBL:ACI10366.1};
KW   Hydrolase {ECO:0000313|EMBL:ACI10366.1};
KW   Metal-binding {ECO:0000256|PIRSR:PIRSR001021-2}.
FT   DOMAIN          4..402
FT                   /note="Glycosyl hydrolase family 13 catalytic"
FT                   /evidence="ECO:0000259|SMART:SM00642"
FT   ACT_SITE        235
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001021-1"
FT   ACT_SITE        265
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001021-1"
FT   BINDING         104
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001021-2"
FT   BINDING         198
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001021-2"
FT   BINDING         206
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001021-2"
FT   BINDING         239
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001021-2"
SQ   SEQUENCE   495 AA;  56233 MW;  5350B006EB7C95CC CRC64;
     MKNPTLLQCF HWYYPAGGEL WREVEALAPS LNEIGINMVW LPPAYKGASG GYSVGYDTYD
     LFDLGEFDQK GSVATKYGDK AQLLAAMKAL KEHDIAVLLD VVVNHKMGAD EKETLQVQRV
     DEHDRTQIAE EIIECEAWTR YTFPVRAGQY SQFVWDYKCF SGIDHIENPT EDGVFKIVND
     YTGEGWNEQV DDELGNFDYL MGANIDFRNH AVTEEIKYWA RWVMEQTGCD GFRLDAVKHI
     PAWFYKAWIE HVQEVAPQPL FIVAEYWSHE VEKLQQYIDL VEGKTMLFDA PLQMKFHEAS
     RQGRDYDMSQ IFSGTLVEAD PFHAVTLVTN HDTQPLQALE APVEPWFKPL AYALILLREN
     GVPSVFYADL FGASYEDTGG DGQTYAIEMP VIEQLHELID ARQRFAHGVQ TLWFDHPNCI
     AFSRSGTDED PGCVVVMSNG DEGEKTLTLG ENYGNKRWRD FLGNREEIVE TDAEGSAIFT
     CNGGSVSVWV MEAVL
//