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B5XP99 (THIM_KLEP3) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 38. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Hydroxyethylthiazole kinase

EC=2.7.1.50
Alternative name(s):
4-methyl-5-beta-hydroxyethylthiazole kinase
Short name=TH kinase
Short name=Thz kinase
Gene names
Name:thiM
Ordered Locus Names:KPK_1618
OrganismKlebsiella pneumoniae (strain 342) [Complete proteome] [HAMAP]
Taxonomic identifier507522 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeKlebsiella

Protein attributes

Sequence length257 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + 4-methyl-5-(2-hydroxyethyl)thiazole = ADP + 4-methyl-5-(2-phosphonooxyethyl)thiazole. HAMAP-Rule MF_00228

Cofactor

Magnesium By similarity. HAMAP-Rule MF_00228

Pathway

Cofactor biosynthesis; thiamine diphosphate biosynthesis; 4-methyl-5-(2-phosphoethyl)-thiazole from 5-(2-hydroxyethyl)-4-methylthiazole: step 1/1. HAMAP-Rule MF_00228

Sequence similarities

Belongs to the Thz kinase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 257257Hydroxyethylthiazole kinase HAMAP-Rule MF_00228
PRO_1000100416

Sites

Binding site491Substrate; via amide nitrogen By similarity
Binding site1241ATP By similarity
Binding site1701ATP By similarity
Binding site1971Substrate; via amide nitrogen By similarity

Sequences

Sequence LengthMass (Da)Tools
B5XP99 [UniParc].

Last modified November 25, 2008. Version 1.
Checksum: 5C71C2F0D9F9A382

FASTA25726,175
        10         20         30         40         50         60 
MPELLNPAPV AHLRHLLRAH SPLVHCMTND VVQTFTANVL LAVGASPAMV IDPREAAQFA 

        70         80         90        100        110        120 
AIADALLINV GTLTEDRAVA MRAAVEHARQ AGKPWTLDPV AVGALTVRTA FCHELLALQP 

       130        140        150        160        170        180 
AAIRGNASEI LALAGMSAGG RGVDTTDTAA AALPAAQALA RRLATVVAVT GEVDYVTDGE 

       190        200        210        220        230        240 
RVLSVAGGNP LMTRVVGTGC ALSAVVAASA ALPGDRLENV AAACGLMKQA GEIAARQGGP 

       250 
GSFIPAFLDA LYQEVQG 

« Hide

References

[1]"Complete genome sequence of the N2-fixing broad host range endophyte Klebsiella pneumoniae 342 and virulence predictions verified in mice."
Fouts D.E., Tyler H.L., DeBoy R.T., Daugherty S., Ren Q., Badger J.H., Durkin A.S., Huot H., Shrivastava S., Kothari S., Dodson R.J., Mohamoud Y., Khouri H., Roesch L.F.W., Krogfelt K.A., Struve C., Triplett E.W., Methe B.A.
PLoS Genet. 4:E1000141-E1000141(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 342.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000964 Genomic DNA. Translation: ACI07032.1.
RefSeqYP_002237469.1. NC_011283.1.

3D structure databases

ProteinModelPortalB5XP99.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING507522.KPK_1618.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACI07032; ACI07032; KPK_1618.
GeneID6935789.
KEGGkpe:KPK_1618.
PATRIC20436760. VBIKlePne121904_1766.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG2145.
HOGENOMHOG000114352.
KOK00878.
OMASPVMAHA.
OrthoDBEOG628F8M.
ProtClustDBPRK09355.

Enzyme and pathway databases

BioCycKPNE507522:GI0B-1619-MONOMER.
UniPathwayUPA00060; UER00139.

Family and domain databases

HAMAPMF_00228. Thz_kinase.
InterProIPR000417. Hyethyz_kinase.
[Graphical view]
PfamPF02110. HK. 1 hit.
[Graphical view]
PIRSFPIRSF000513. Thz_kinase. 1 hit.
PRINTSPR01099. HYETHTZKNASE.
TIGRFAMsTIGR00694. thiM. 1 hit.
ProtoNetSearch...

Entry information

Entry nameTHIM_KLEP3
AccessionPrimary (citable) accession number: B5XP99
Entry history
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: November 25, 2008
Last modified: February 19, 2014
This is version 38 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways