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B5XM63 (DDL_STRPZ) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 43. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase

EC=6.3.2.4
Alternative name(s):
D-Ala-D-Ala ligase
D-alanylalanine synthetase
Gene names
Name:ddl
Ordered Locus Names:Spy49_1137c
OrganismStreptococcus pyogenes serotype M49 (strain NZ131) [Complete proteome] [HAMAP]
Taxonomic identifier471876 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus

Protein attributes

Sequence length348 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 348348D-alanine--D-alanine ligase HAMAP-Rule MF_00047
PRO_1000116640

Regions

Domain132 – 334203ATP-grasp
Nucleotide binding162 – 21756ATP By similarity

Sites

Metal binding2881Magnesium or manganese 1 By similarity
Metal binding3011Magnesium or manganese 1 By similarity
Metal binding3011Magnesium or manganese 2 By similarity
Metal binding3031Magnesium or manganese 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
B5XM63 [UniParc].

Last modified November 25, 2008. Version 1.
Checksum: BC221F78EBD31314

FASTA34838,969
        10         20         30         40         50         60 
MSKQTLVLLY GGRSAEREVS VLSAESVMRA VNYDKFLVKT YFITQMGQFI KTQQFSEKPS 

        70         80         90        100        110        120 
ESERLMTNET IELTQKIKPS DIYEEGAVVF PVLHGPMGED GSIQGFLEVL RMPYIGTNVM 

       130        140        150        160        170        180 
SSSIAMDKIT TKRVLESIGI PQVAYTVYID GQDLEACLVE TLARLTFPIF VKPANMGSSV 

       190        200        210        220        230        240 
GISKAQTKVE LRKAIQLALT YDSRVLIEQG VVAREIEVGL LGNDKVKSTL PGEVIKDVDF 

       250        260        270        280        290        300 
YDYQAKYVDN KITMAIPADV DQSIVTEMRS YAEVAFKALG GCGLSRCDFF LTQDGQVYLN 

       310        320        330        340 
ELNTMPGFTQ WSMYPLLWEN MGLAYPDLIE ELVTLAQEMF DQRESHLI 

« Hide

References

[1]"Genome sequence of a nephritogenic and highly transformable M49 strain of Streptococcus pyogenes."
McShan W.M., Ferretti J.J., Karasawa T., Suvorov A.N., Lin S., Qin B., Jia H., Kenton S., Najar F., Wu H., Scott J., Roe B.A., Savic D.J.
J. Bacteriol. 190:7773-7785(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: NZ131.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000829 Genomic DNA. Translation: ACI61425.1.
RefSeqYP_002286120.1. NC_011375.1.

3D structure databases

ProteinModelPortalB5XM63.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING471876.Spy49_1137c.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACI61425; ACI61425; Spy49_1137c.
GeneID6984372.
KEGGsoz:Spy49_1137c.
PATRIC19757334. VBIStrPyo129711_1174.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1181.
HOGENOMHOG000011593.
KOK01921.
OMAQIDVIFP.
OrthoDBEOG64BQ73.

Enzyme and pathway databases

UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDDL_STRPZ
AccessionPrimary (citable) accession number: B5XM63
Entry history
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: November 25, 2008
Last modified: May 14, 2014
This is version 43 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways