B5XLN9 (GUAA_STRPZ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
GMP synthase [glutamine-hydrolyzing]
EC=6.3.5.2

Alternative name(s):
GMP synthetase
Glutamine amidotransferase

Gene names

Name:	guaA
Ordered Locus Names:	Spy49_0949

Organism

Streptococcus pyogenes serotype M49 (strain NZ131) [Complete proteome] [HAMAP]

Taxonomic identifier

471876 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Lactobacillales › Streptococcaceae › Streptococcus › Streptococcus pyogenes › Streptococcus pyogenes serotype M49

Protein attributes

Sequence length	520 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the synthesis of GMP from XMP By similarity.
Catalytic activity	ATP + xanthosine 5'-phosphate + L-glutamine + H2O = AMP + diphosphate + GMP + L-glutamate. HAMAP-Rule MF_00344
Pathway	Purine metabolism; GMP biosynthesis; GMP from XMP (L-Gln route): step 1/1. HAMAP-Rule MF_00344
Subunit structure	Homodimer By similarity.
Sequence similarities	Contains 1 glutamine amidotransferase type-1 domain. Contains 1 GMPS ATP-PPase (ATP pyrophosphatase) domain.

Ontologies

Keywords
Biological process	GMP biosynthesis Purine biosynthesis
Domain	Glutamine amidotransferase
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	GMP biosynthetic process Inferred from electronic annotation. Source: HAMAP glutamine metabolic process Inferred from electronic annotation. Source: HAMAP
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP GMP synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: HAMAP pyrophosphatase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 520	520	GMP synthase [glutamine-hydrolyzing] HAMAP-Rule MF_00344		PRO_1000120437
Regions
	Domain	12 – 205	194	Glutamine amidotransferase type-1
	Domain	206 – 395	190	GMPS ATP-PPase
	Nucleotide binding	233 – 239	7	ATP By similarity
Sites
	Active site	89	1	Nucleophile By similarity
	Active site	179	1	By similarity
	Active site	181	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	B5XLN9 [UniParc]. Last modified November 25, 2008. Version 1. Checksum: 49B94153591CBB78	FASTA	520	57,502
	10 20 30 40 50 60 MTEISILNDV QKIIVLDYGS QYNQLIARRI REFGVFSELK SHKITAQELR EINPIGIVLS 70 80 90 100 110 120 GGPNSVYADN AFGIDPEIFE LGIPILGICY GMQLITHKLG GKVVPAGQAG NREYGQSTLH 130 140 150 160 170 180 LRETSKLFSG TPQEQLVLMS HGDAVTEIPE GFHLVGDSND CPYAAIENTE KNLYGIQFHP 190 200 210 220 230 240 EVRHSVYGND ILKNFAISIC GARGDWSMDN FIDMEIAKIR ETVGDRKVLL GLSGGVDSSV 250 260 270 280 290 300 VGVLLQKAIG DQLTCIFVDH GLLRKDEGDQ VMGMLGGKFG LNIIRVDASK RFLDLLADVE 310 320 330 340 350 360 DPEKKRKIIG NEFVYVFDDE ASKLKGVDFL AQGTLYTDII ESGTETAQTI KSHHNVGGLP 370 380 390 400 410 420 EDMQFELIEP LNTLFKDEVR ALGIALGMPE EIVWRQPFPG PGLAIRVMGA ITEEKLETVR 430 440 450 460 470 480 ESDAILREEI AKAGLDRDVW QYFTVNTGVR SVGVMGDGRT YDYTIAIRAI TSIDGMTADF 490 500 510 520 AQLPWDVLKK ISTRIVNEVD HVNRIVYDIT SKPPATVEWE

References

[1]

"Genome sequence of a nephritogenic and highly transformable M49 strain of Streptococcus pyogenes." McShan W.M., Ferretti J.J., Karasawa T., Suvorov A.N., Lin S., Qin B., Jia H., Kenton S., Najar F., Wu H., Scott J., Roe B.A., Savic D.J. J. Bacteriol. 190:7773-7785(2008) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NZ131.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000829 Genomic DNA. Translation: ACI61251.1.
RefSeq	YP_002285946.1. NC_011375.1.
3D structure databases
ProteinModelPortal	B5XLN9.
ModBase	Search...
Protein-protein interaction databases
STRING	471876.Spy49_0949.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACI61251; ACI61251; Spy49_0949.
GeneID	6985945.
KEGG	soz:Spy49_0949.
PATRIC	19756959. VBIStrPyo129711_0987.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0519.
HOGENOM	HOG000223964.
KO	K01951.
OMA	TCMFIDH.
ProtClustDB	PRK00074.
Enzyme and pathway databases
UniPathway	UPA00189; UER00296.
Family and domain databases
Gene3D	3.40.50.620. 1 hit.
HAMAP	MF_00344. GMP_synthase.
InterPro	IPR017926. GATASE_1. IPR001674. GMP_synth_C. IPR004739. GMP_synth_N. IPR022955. GMP_synthase. IPR025777. GMPS_ATP_PPase_dom. IPR022310. NAD/GMP_synthase. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Pfam	PF00117. GATase. 1 hit. PF00958. GMP_synt_C. 1 hit. PF02540. NAD_synthase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00884. guaA_Cterm. 1 hit. TIGR00888. guaA_Nterm. 1 hit.
PROSITE	PS51273. GATASE_TYPE_1. 1 hit. PS51553. GMPS_ATP_PPASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUAA_STRPZ

Accession

Primary (citable) accession number: B5XLN9

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	November 25, 2008
Last modified:	May 1, 2013
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program