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Protein

Glutamate--tRNA ligase

Gene

gltX

Organism
Streptococcus pyogenes serotype M49 (strain NZ131)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).UniRule annotation

Catalytic activityi

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei258 – 2581ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate--tRNA ligaseUniRule annotation (EC:6.1.1.17UniRule annotation)
Alternative name(s):
Glutamyl-tRNA synthetaseUniRule annotation
Short name:
GluRSUniRule annotation
Gene namesi
Name:gltXUniRule annotation
Ordered Locus Names:Spy49_0203
OrganismiStreptococcus pyogenes serotype M49 (strain NZ131)
Taxonomic identifieri471876 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 496496Glutamate--tRNA ligasePRO_1000090115Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliB5XJN3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi11 – 2111"HIGH" regionAdd
BLAST
Motifi255 – 2595"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0008.
HOGENOMiHOG000252720.
KOiK09698.
OMAiKLNWINS.
OrthoDBiEOG6DRPF7.

Family and domain databases

Gene3Di1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00022_B. Glu_tRNA_synth_B.
InterProiIPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR001412. aa-tRNA-synth_I_CS.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERiPTHR10119. PTHR10119. 1 hit.
PfamiPF00749. tRNA-synt_1c. 1 hit.
[Graphical view]
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF48163. SSF48163. 1 hit.
TIGRFAMsiTIGR00464. gltX_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B5XJN3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKPIRVRYA PSPTGLLHIG NARTALFNYL YARRHGGTFI IRIEDTDRKR
60 70 80 90 100
HVEDGERSQL ENLKWLGMDW DESPETHENY RQSERLALYQ QYIDQLLAEG
110 120 130 140 150
KAYKSYVTEE ELAAERERQE AAGETPRYIN EFIGMSADEK AKYIAEREAA
160 170 180 190 200
GIVPTVRLAV NESGIYKWTD MVKGDIEFEG GNIGGDWVIQ KKDGYPTYNF
210 220 230 240 250
AVVVDDHDMQ ISHVIRGDDH IANTPKQLMV YEALGWEAPE FGHMTLIINS
260 270 280 290 300
ETGKKLSKRD TNTLQFIEDY RKKGYMPEAV FNFIALLGWN PGGEEEIFSR
310 320 330 340 350
EQLIALFDEN RLSKSPAAFD QKKMDWMSNE YLKHADFETV YALCKPFLEE
360 370 380 390 400
AGRLTEKAEK LVELYKPQLK SADEIIPLTD LFFSDFPELT EAEKEVMAGE
410 420 430 440 450
TVSTVLQAFK AKLEAMSDED FKPENIFPQI KAVQKETGIK GKNLFMPIRI
460 470 480 490
AVSGEMHGPE LPNTIYLLGR DKSIEHIKNH AIRLYRSPLF LNALRS
Length:496
Mass (Da):56,858
Last modified:November 25, 2008 - v1
Checksum:i9E9097CBE434624D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000829 Genomic DNA. Translation: ACI60545.1.
RefSeqiWP_012560429.1. NC_011375.1.
YP_002285240.1. NC_011375.1.

Genome annotation databases

EnsemblBacteriaiACI60545; ACI60545; Spy49_0203.
KEGGisoz:Spy49_0203.
PATRICi19755366. VBIStrPyo129711_0202.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000829 Genomic DNA. Translation: ACI60545.1.
RefSeqiWP_012560429.1. NC_011375.1.
YP_002285240.1. NC_011375.1.

3D structure databases

ProteinModelPortaliB5XJN3.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACI60545; ACI60545; Spy49_0203.
KEGGisoz:Spy49_0203.
PATRICi19755366. VBIStrPyo129711_0202.

Phylogenomic databases

eggNOGiCOG0008.
HOGENOMiHOG000252720.
KOiK09698.
OMAiKLNWINS.
OrthoDBiEOG6DRPF7.

Family and domain databases

Gene3Di1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00022_B. Glu_tRNA_synth_B.
InterProiIPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR001412. aa-tRNA-synth_I_CS.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERiPTHR10119. PTHR10119. 1 hit.
PfamiPF00749. tRNA-synt_1c. 1 hit.
[Graphical view]
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF48163. SSF48163. 1 hit.
TIGRFAMsiTIGR00464. gltX_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Genome sequence of a nephritogenic and highly transformable M49 strain of Streptococcus pyogenes."
    McShan W.M., Ferretti J.J., Karasawa T., Suvorov A.N., Lin S., Qin B., Jia H., Kenton S., Najar F., Wu H., Scott J., Roe B.A., Savic D.J.
    J. Bacteriol. 190:7773-7785(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: NZ131.

Entry informationi

Entry nameiSYE_STRPZ
AccessioniPrimary (citable) accession number: B5XJN3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: November 25, 2008
Last modified: June 24, 2015
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.