Reviewed, UniProtKB/Swiss-Prot B5X8M4 (BRCC3_SALSA)

Names and origin

Protein names

Recommended name:
    Lys-63-specific deubiquitinase BRCC36
    EC=3.1.2.15
Alternative name(s):
    BRCA1-A complex subunit BRCC36
    BRISC complex subunit BRCC36
    BRCA1/BRCA2-containing complex subunit 3
    BRCA1/BRCA2-containing complex subunit 36

Gene names

Name:	brcc3
Synonyms:	brcc36

Organism

Salmo salar (Atlantic salmon)

Taxonomic identifier

8030 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Euteleostei › Protacanthopterygii › Salmoniformes › Salmonidae › Salmoninae › Salmo

Protein attributes

Sequence length	260 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Metalloprotease that specifically cleaves 'Lys-63'-linked polyubiquitin chains. Does not have activity toward 'Lys-48'-linked polyubiquitin chains. Component of the BRCA1-A complex, a complex that specifically recognizes 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the brca1-bard1 heterodimer to sites of DNA damage at double-strand breaks (DSBs). In the BRCA1-A complex, it specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX, antagonizing the rnf8-dependent ubiquitination at double-strand breaks (DSBs). Catalytic subunit of the BRISC complex, a multiprotein complex that specifically cleaves 'Lys-63'-linked ubiquitin in various substrates. Mediates the specific 'Lys-63'-specific deubiquitination associated with the COP9 signalosome complex (CSN), via the interaction of the BRISC complex with the CSN complex By similarity.
Catalytic activity	Ubiquitin C-terminal thioester + H2O = ubiquitin + a thiol.
Subunit structure	Component of the BRCA1-A complex. Component of the BRISC complex By similarity.
Subcellular location	Nucleus By similarity. Note: Localizes at sites of DNA damage at double-strand breaks (DSBs) By similarity.
Sequence similarities	Belongs to the peptidase M67A family. BRCC36 subfamily. Contains 1 MPN (JAB/Mov34) domain.

Ontologies

Keywords
Biological process	DNA damage DNA repair Ubl conjugation pathway
Cellular component	Nucleus
Ligand	Metal-binding Zinc
Molecular function	Chromatin regulator Hydrolase Metalloprotease Protease
Gene Ontology (GO)
Biological process	G2/M transition DNA damage checkpoint Inferred from sequence or structural similarity. Source: UniProtKB double-strand break repair Inferred from sequence or structural similarity. Source: UniProtKB histone H2A K63-linked deubiquitination Inferred from sequence or structural similarity. Source: UniProtKB modification-dependent protein catabolic process Inferred from electronic annotation. Source: UniProtKB-KW positive regulation of DNA repair Inferred from sequence or structural similarity. Source: UniProtKB response to ionizing radiation Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	BRCA1-A complex Inferred from sequence or structural similarity. Source: UniProtKB BRISC complex Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	metallopeptidase activity Inferred from sequence or structural similarity. Source: UniProtKB polyubiquitin binding Inferred from sequence or structural similarity. Source: UniProtKB ubiquitin thiolesterase activity Inferred from sequence or structural similarity. Source: UniProtKB ubiquitin-specific protease activity Inferred from sequence or structural similarity. Source: UniProtKB zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 260	260	Lys-63-specific deubiquitinase BRCC36		PRO_0000373948
Regions
	Motif	92 – 105	14	JAMM motif
Sites
	Metal binding	92	1	Zinc; catalytic By similarity
	Metal binding	94	1	Zinc; catalytic By similarity
	Metal binding	105	1	Zinc; catalytic By similarity

Sequences

	Sequence		Length	Mass (Da)
	B5X8M4-1 [UniParc]. Last modified November 25, 2008. Version 1. Checksum: 8B88ECF564F389CA	FASTA	260	29,661
	10 20 30 40 50 60 MAVSAVHLES DAFLVCMNHA LSTEKEEVMG LCIGEVDTNR IVHIHSVIIL RRSDKRKDRV 70 80 90 100 110 120 EISPEQLSSA ATEAERLAEM TGRPMRVVGW YHSHPHITVW PSHVDVRTQA MYQMMDQGFV 130 140 150 160 170 180 GLIFSCFIED KNTKTGRVLY TCFQSVQAQK GSEYERIEIP IHVVPHEAIG KVCLESAVEL 190 200 210 220 230 240 PRILCQEEQD TYRKIHSLTH LDPITKIHNG SVFTKNLCSQ MSAVSGPLLQ WLEDRLEQNR 250 260 QSVIELQLEK ERLTQELATM

References

[1]	"Salmo salar full-length cDNAs." Leong J., von Schalburg K., Cooper G.A., Moore R., Holt R., Davidson W.S., Koop B.F. Submitted (OCT-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BT047393 mRNA. Translation: ACI67194.1.
RefSeq	NP_001134356.1.
UniGene	Ssa.10060
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	100195855.
Organism-specific databases
CTD	100195855.
Family and domain databases
InterPro	IPR000555. Mov34_MPN_PAD1. [Graphical view]
Pfam	PF01398. Mov34. 1 hit. [Graphical view]
SMART	SM00232. JAB_MPN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BRCC3_SALSA

Accession

Primary (citable) accession number: B5X8M4

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 5, 2009
Last sequence update:	November 25, 2008
Last modified:	December 15, 2009
This is version 9 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)