B5X3C1 (GALC_SALSA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Galactocerebrosidase
Short name=GALCERase
EC=3.2.1.46

Alternative name(s):
Galactosylceramidase

Gene names

Name:

galc

Organism

Salmo salar (Atlantic salmon)

Taxonomic identifier

8030 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Euteleostei › Protacanthopterygii › Salmoniformes › Salmonidae › Salmoninae › Salmo

Protein attributes

Sequence length	666 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Hydrolyzes the galactose ester bonds of galactosylceramide, galactosylsphingosine, lactosylceramide, and monogalactosyldiglyceride By similarity.
Catalytic activity	D-galactosyl-N-acylsphingosine + H2O = D-galactose + N-acylsphingosine.
Subcellular location	Lysosome By similarity.
Sequence similarities	Belongs to the glycosyl hydrolase 59 family.

Ontologies

Keywords
Cellular component	Lysosome
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro galactosylceramide catabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	lysosome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	cation binding Inferred from electronic annotation. Source: InterPro galactosylceramidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 22	22	Potential
	Chain	23 – 666	644	Galactocerebrosidase		PRO_0000370714
Amino acid modifications
	Glycosylation	125	1	N-linked (GlcNAc...) Potential
	Glycosylation	361	1	N-linked (GlcNAc...) Potential
	Glycosylation	385	1	N-linked (GlcNAc...) Potential
	Glycosylation	390	1	N-linked (GlcNAc...) Potential
	Glycosylation	500	1	N-linked (GlcNAc...) Potential
	Glycosylation	540	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	B5X3C1 [UniParc]. Last modified November 25, 2008. Version 1. Checksum: 7169F6D7ADD8CA67	FASTA	666	74,494
	10 20 30 40 50 60 MIYKLYFAIA LCFSLCFDLC IAESYVLDDK VGLGRTFDGI GGLSGGGATS RLLVNYAEPY 70 80 90 100 110 120 RSQILDYLFK PNFGASLHIL KVEIGGDAQT TDGTEPSHMH YENDENFFRG YEWWLMREAK 130 140 150 160 170 180 KRNPNITLIG LPWAFPGWVG HGKNWPYDFP DITASYVVSW ILGAKHYHDL NIDYVGIWNE 190 200 210 220 230 240 RNFDSKYIKL LRYTLDKSGL ERVRIIASDN LWQPITYSLC VDQELADAVD VIGAHYPGTT 250 260 270 280 290 300 TVIEALKTQK KLWSSEDYST FNDEVGGGCW ARILNQNYVN GLMTATISWN LVASYYEDLP 310 320 330 340 350 360 FGRDGLMTAE EPWTGNYVVE SPIWITAHTT QFSQPGWTYL QTVGHLVHGG SYVALTDSNG 370 380 390 400 410 420 NLTVVIETMT HDHSVCIRPP LLPFNVTAQN VTFQLKGSFA LIKELQVWQS RFDFKTKKPF 430 440 450 460 470 480 FFKKLSPLKI SDGSFTLSLD VDEVYTLTTI STGLKGTYPD PPTSGPFPKV YFDDFNVANP 490 500 510 520 530 540 SFSEAPDFAD QTGVFEYYIN LTDPGPHVFT LRQVVTQMPV TWATDADQTI SVIGDYKWQN 550 560 570 580 590 600 LTVTCDVFME TVKTGGVFIA ARVDKGGQSV RSAKGVFFWV FADGSYKVTN DLVGKTVLAE 610 620 630 640 650 660 GLSGTRAYGW HTLTLTVEGQ YATGLLNGYP LWKDAVVLGP KNGWAAIGTH SFELAQFDNF AVEAKL

References

[1]

"Salmo salar and Esox lucius full-length cDNA sequences reveal changes in evolutionary pressures on a post-tetraploidization genome." Leong J.S., Jantzen S.G., von Schalburg K.R., Cooper G.A., Messmer A.M., Liao N.Y., Munro S., Moore R., Holt R.A., Jones S.J., Davidson W.S., Koop B.F. BMC Genomics 11:279-279(2010) [PubMed: 20433749] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BT045540 mRNA. Translation: ACI33802.1.
RefSeq	NP_001133732.1. NM_001140260.1.
UniGene	Ssa.15050.
3D structure databases
ProteinModelPortal	B5X3C1.
ModBase	Search...
Protein family/group databases
CAZy	GH59. Glycoside Hydrolase Family 59.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	100195231.
Organism-specific databases
CTD	2581.
Family and domain databases
InterPro	IPR001286. Glyco_hydro_59. IPR013781. Glyco_hydro_subgr_catalytic. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
PANTHER	PTHR15172. Glyco_hydro_59. 1 hit.
Pfam	PF02057. Glyco_hydro_59. 1 hit. [Graphical view]
PRINTS	PR00850. GLHYDRLASE59.
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GALC_SALSA

Accession

Primary (citable) accession number: B5X3C1

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 5, 2009
Last sequence update:	November 25, 2008
Last modified:	June 28, 2011
This is version 14 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program