Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

ATP-binding cassette sub-family B member 5

Gene

Abcb5

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Drug efflux transporter present in a number of stem cells that acts as a regulator of cellular differentiation. Able to mediate efflux from cells of the rhodamine dye and of the therapeutic drug doxorubicin. Specifically present in limbal stem cells, where it plays a key role in corneal development and repair.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi422 – 4298ATPPROSITE-ProRule annotation
Nucleotide bindingi1051 – 10588ATPPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

  • cell differentiation Source: UniProtKB-KW
  • compound eye corneal lens development Source: UniProtKB
  • regulation of membrane potential Source: MGI
  • transmembrane transport Source: MGI
Complete GO annotation...

Keywords - Biological processi

Differentiation, Transport

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-382556. ABC-family proteins mediated transport.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-binding cassette sub-family B member 5
Alternative name(s):
ABCB5 P-gp
P-glycoprotein ABCB5
Gene namesi
Name:Abcb5
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 12

Organism-specific databases

MGIiMGI:1924956. Abcb5.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei46 – 6621HelicalPROSITE-ProRule annotationAdd
BLAST
Transmembranei104 – 12421HelicalPROSITE-ProRule annotationAdd
BLAST
Transmembranei290 – 31021HelicalPROSITE-ProRule annotationAdd
BLAST
Transmembranei314 – 33421HelicalPROSITE-ProRule annotationAdd
BLAST
Transmembranei694 – 71421HelicalPROSITE-ProRule annotationAdd
BLAST
Transmembranei738 – 75821HelicalPROSITE-ProRule annotationAdd
BLAST
Transmembranei814 – 83623HelicalPROSITE-ProRule annotationAdd
BLAST
Transmembranei841 – 86323HelicalPROSITE-ProRule annotationAdd
BLAST
Transmembranei955 – 97521HelicalPROSITE-ProRule annotationAdd
BLAST

GO - Cellular componenti

  • integral component of plasma membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Defects in corneal development. While eyes contain all anterior and posterior segment components, corneas show developmental abnormalities characterized by decreased cellularity of the apical epithelial layer and disorganized basal and wing cell layers. Defects are due to depletion of quiescent limbal stem cells, leading to enhanced proliferation and apoptosis, and resulting in defective corneal differentiation and wound healing.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 12551255ATP-binding cassette sub-family B member 5PRO_0000430433Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi86 – 861N-linked (GlcNAc...)Sequence analysis
Glycosylationi92 – 921N-linked (GlcNAc...)Sequence analysis
Glycosylationi189 – 1891N-linked (GlcNAc...)Sequence analysis
Glycosylationi372 – 3721N-linked (GlcNAc...)Sequence analysis
Glycosylationi391 – 3911N-linked (GlcNAc...)Sequence analysis
Glycosylationi643 – 6431N-linked (GlcNAc...)Sequence analysis
Glycosylationi790 – 7901N-linked (GlcNAc...)Sequence analysis
Glycosylationi1036 – 10361N-linked (GlcNAc...)Sequence analysis
Glycosylationi1105 – 11051N-linked (GlcNAc...)Sequence analysis
Glycosylationi1189 – 11891N-linked (GlcNAc...)Sequence analysis
Glycosylationi1229 – 12291N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

EPDiB5X0E4.
PaxDbiB5X0E4.

Expressioni

Tissue specificityi

In developing eye, expressed in basal limbal epithelium but not in central cornea. Acts as a marker of limbal stem cells.1 Publication

Interactioni

Protein-protein interaction databases

IntActiB5X0E4. 1 interaction.
STRINGi10090.ENSMUSP00000046177.

Structurei

3D structure databases

ProteinModelPortaliB5X0E4.
SMRiB5X0E4. Positions 37-624, 675-1252.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini51 – 351301ABC transmembrane type-1 1PROSITE-ProRule annotationAdd
BLAST
Domaini387 – 623237ABC transporter 1PROSITE-ProRule annotationAdd
BLAST
Domaini694 – 981288ABC transmembrane type-1 2PROSITE-ProRule annotationAdd
BLAST
Domaini1016 – 1254239ABC transporter 2PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 2 ABC transmembrane type-1 domains.PROSITE-ProRule annotation
Contains 2 ABC transporter domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0055. Eukaryota.
COG1132. LUCA.
GeneTreeiENSGT00530000062896.
HOVERGENiHBG080809.
InParanoidiB5X0E4.
KOiK05660.
OMAiWFDGCDV.
OrthoDBiEOG7Z3F4H.
PhylomeDBiB5X0E4.
TreeFamiTF105193.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR003593. AAA+_ATPase.
IPR011527. ABC1_TM_dom.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00664. ABC_membrane. 2 hits.
PF00005. ABC_tran. 2 hits.
[Graphical view]
SMARTiSM00382. AAA. 2 hits.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
SSF90123. SSF90123. 2 hits.
PROSITEiPS50929. ABC_TM1F. 2 hits.
PS00211. ABC_TRANSPORTER_1. 2 hits.
PS50893. ABC_TRANSPORTER_2. 2 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: B5X0E4-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MANSERTNGL QETNQRYGPL QEQVPKVGNQ AVGPIEIFRF ADNLDIVLMT
60 70 80 90 100
LGILASMING ATVPLMSLVL GEISDHLING CLVQTNRTKY QNCSQTQEKL
110 120 130 140 150
NEDIIVLTLY YIGIGAAALI FGYVQISFWV ITAARQTTRI RKQFFHSILA
160 170 180 190 200
QDISWFDGSD ICELNTRMTG DINKLCDGIG DKIPLMFQNI SGFSIGLVIS
210 220 230 240 250
LIKSWKLSLV VLSTSPLIMA SSALCSRMII SLTSKELDAY SKAGAVAEEA
260 270 280 290 300
LSSIQTVTAF GAQEKEIQRY TQHLKDAKDA GIKRATASKL SLGAVYFFMN
310 320 330 340 350
GAYGLAFWYG TSLIFGGEPG YTIGTILAVF FSVIHSSYCI GSVAPHLETF
360 370 380 390 400
TVARGAAFNI FQVIDKKPNI DNFSTAGFVP ECIEGNIEFK NVSFSYPSRP
410 420 430 440 450
SAKVLKGLNL KIKAGETVAL VGPSGSGKST TVQLLQRLYD PEDGCITVDE
460 470 480 490 500
NDIRAQNVRH YREQIGVVRQ EPVLFGTTIG NNIKFGREGV GEKEMEQAAR
510 520 530 540 550
EANAYDFIMA FPKKFNTLVG EKGAQMSGGQ KQRIAIARAL VRNPKILILD
560 570 580 590 600
EATSALDTES ESLVQTALEK ASKGRTTIVV AHRLSTIRGA DLIVTMKDGM
610 620 630 640 650
VVEKGTHAEL MAKQGLYYSL AMAQDIKKVD EQMESRTCST AGNASYGSLC
660 670 680 690 700
DVNSAKAPCT DQLEEAVHHQ KTSLPEVSLL KIFKLSKSEW PFVVLGTLAS
710 720 730 740 750
ALNGSVHPVF SIIFGKLVTM FEDKNKATLK QDAELYSMML VVLGIVALVT
760 770 780 790 800
YLMQGLFYGR AEENLAMRLR HSAFKAMLYQ DMAWYDDKEN NTGALTTTLA
810 820 830 840 850
VDVAQIQGAA TSRLGIVTQD VSNMSLSILI SFIYGWEMTL LILSFAPVLA
860 870 880 890 900
VTGMIQTAAM AGFANRDKQA LKRAGKIATE AVENIRTVVS LTRERAFEQM
910 920 930 940 950
YEETLQTQHR NALKRAHITG CCYAVSHAFV HFAHAAGFRF GAYLIQAGRM
960 970 980 990 1000
MPEGMFIVFT AIAYGAMAIG ETLVWAPEYS KAKAGASHLF ALLKNKPTIN
1010 1020 1030 1040 1050
SCSQSGEKPD TCEGNLEFRE VSFVYPCRPE VPVLQNMSLS IEKGKTVAFV
1060 1070 1080 1090 1100
GSSGCGKSTC VQLLQRFYDP MKGQVLLDGV DVKELNVQWL RSQTAIVSQE
1110 1120 1130 1140 1150
PVLFNCSIAE NIAYGDNSRM VPLEEIKEVA DAANIHSFIE GLPRKYNTLV
1160 1170 1180 1190 1200
GLRGVQLSGG QKQRLAIARA LLRKPKILLL DEATSALDNE SEKVVQQALD
1210 1220 1230 1240 1250
KARRGKTCLV VAHRLSTIQN ADMIVVLQNG SIKEQGTHQE LLRNGDTYFK

LVAAH
Length:1,255
Mass (Da):137,397
Last modified:November 25, 2008 - v1
Checksum:i481BA90341116BC6
GO
Isoform 2 (identifier: B5X0E4-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-525: Missing.

Show »
Length:730
Mass (Da):80,013
Checksum:i4FE7A189C3DA5ECA
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti564 – 5641V → I in AFS60113 (PubMed:25030174).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 525525Missing in isoform 2. 1 PublicationVSP_056757Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY766239 mRNA. Translation: AAX11686.1.
JQ655148 mRNA. Translation: AFS60113.1.
AC126277 Genomic DNA. No translation available.
AC131717 Genomic DNA. No translation available.
CCDSiCCDS49198.1. [B5X0E4-1]
RefSeqiNP_084237.1. NM_029961.2. [B5X0E4-1]
UniGeneiMm.261496.

Genome annotation databases

EnsembliENSMUST00000035515; ENSMUSP00000046177; ENSMUSG00000072791. [B5X0E4-1]
GeneIDi77706.
KEGGimmu:77706.
UCSCiuc007pij.2. mouse. [B5X0E4-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY766239 mRNA. Translation: AAX11686.1.
JQ655148 mRNA. Translation: AFS60113.1.
AC126277 Genomic DNA. No translation available.
AC131717 Genomic DNA. No translation available.
CCDSiCCDS49198.1. [B5X0E4-1]
RefSeqiNP_084237.1. NM_029961.2. [B5X0E4-1]
UniGeneiMm.261496.

3D structure databases

ProteinModelPortaliB5X0E4.
SMRiB5X0E4. Positions 37-624, 675-1252.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiB5X0E4. 1 interaction.
STRINGi10090.ENSMUSP00000046177.

Proteomic databases

EPDiB5X0E4.
PaxDbiB5X0E4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000035515; ENSMUSP00000046177; ENSMUSG00000072791. [B5X0E4-1]
GeneIDi77706.
KEGGimmu:77706.
UCSCiuc007pij.2. mouse. [B5X0E4-1]

Organism-specific databases

CTDi340273.
MGIiMGI:1924956. Abcb5.

Phylogenomic databases

eggNOGiKOG0055. Eukaryota.
COG1132. LUCA.
GeneTreeiENSGT00530000062896.
HOVERGENiHBG080809.
InParanoidiB5X0E4.
KOiK05660.
OMAiWFDGCDV.
OrthoDBiEOG7Z3F4H.
PhylomeDBiB5X0E4.
TreeFamiTF105193.

Enzyme and pathway databases

ReactomeiR-MMU-382556. ABC-family proteins mediated transport.

Miscellaneous databases

PROiB5X0E4.
SOURCEiSearch...

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR003593. AAA+_ATPase.
IPR011527. ABC1_TM_dom.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00664. ABC_membrane. 2 hits.
PF00005. ABC_tran. 2 hits.
[Graphical view]
SMARTiSM00382. AAA. 2 hits.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
SSF90123. SSF90123. 2 hits.
PROSITEiPS50929. ABC_TM1F. 2 hits.
PS00211. ABC_TRANSPORTER_1. 2 hits.
PS50893. ABC_TRANSPORTER_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
    Strain: BALB/cJ.
    Tissue: Testis.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.

Entry informationi

Entry nameiABCB5_MOUSE
AccessioniPrimary (citable) accession number: B5X0E4
Secondary accession number(s): W5QLL5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 2014
Last sequence update: November 25, 2008
Last modified: June 8, 2016
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.