Reviewed, UniProtKB/Swiss-Prot B5VSZ6 (GPI3_YEAS6)

Names and origin

Protein names

Recommended name:
    Phosphatidylinositol N-acetylglucosaminyltransferase GPI3 subunit
    EC=2.4.1.198
Alternative name(s):
    GlcNAc-PI synthesis protein

Gene names

Name:	SPT14
ORF Names:	AWRI1631_160910

Organism

Saccharomyces cerevisiae (strain AWRI1631) (Baker's yeast)

Taxonomic identifier

545124 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	452 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalytic subunit in the complex catalyzing the transfer of N-acetylglucosamine from UDP-N-acetylglucosamine to phosphatidylinositol, the first step of GPI biosynthesis By similarity.
Catalytic activity	UDP-N-acetyl-D-glucosamine + 1-phosphatidyl-1D-myo-inositol = UDP + 6-(N-acetyl-alpha-D-glucosaminyl)-1-phosphatidyl-1D-myo-inositol.
Enzyme regulation	Inhibited by Ras, probably via the interaction between RAS2 and ERI1 By similarity.
Pathway	Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.
Subunit structure	Component of the phosphatidylinositol N-acetylglucosaminyltransferase complex composed of at least GPI1, GPI2, GPI3, GPI15, GPI19 and ERI1 By similarity.
Subcellular location	Endoplasmic reticulum membrane; Single-pass membrane protein By similarity.
Sequence similarities	Belongs to the glycosyltransferase 1 family.
Sequence caution	The sequence EDZ68944.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	GPI-anchor biosynthesis
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane
Molecular function	Glycosyltransferase Transferase
Gene Ontology (GO)
Biological process	GPI anchor biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	phosphatidylinositol N-acetylglucosaminyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 452	452	Phosphatidylinositol N-acetylglucosaminyltransferase GPI3 subunit		PRO_0000377638
Regions
	Transmembrane	407 – 427	21	Potential

Sequences

	Sequence		Length	Mass (Da)
	B5VSZ6-1 [UniParc]. Last modified June 16, 2009. Version 2. Checksum: 7589FD815707EC73	FASTA	452	51,242
	10 20 30 40 50 60 MGFNIAMLCD FFYPQLGGVE FHIYHLSQKL IDLGHSVVII THAYKDRVGV RHLTNGLKVY 70 80 90 100 110 120 HVPFFVIFRE TTFPTVFSTF PIIRNILLRE QIQIVHSHGS ASTFAHEGIL HANTMGLRTV 130 140 150 160 170 180 FTDHSLYGFN NLTSIWVNKL LTFTLTNIDR VICVSNTCKE NMIVRTELSP DIISVIPNAV 190 200 210 220 230 240 VSEDFKPRDP TGGTKRKQSR DKIVIVVIGR LFPNKGSDLL TRIIPKVCSS HEDVEFIVAG 250 260 270 280 290 300 DGPKFIDFQQ MIESHRLQKR VQLLGSVPHE KVRDVLCQGD IYLHASLTEA FGTILVEAAS 310 320 330 340 350 360 CNLLIVTTQV GGIPEVLPNE MTVYAEQTSV SDLVQATNKA INIIRSKALD TSSFHDSVSK 370 380 390 400 410 420 MYDWMDVAKR TVEIYTNISS TSSADDKDWM KMVANLYKRD GIWAKHLYLL CGIVEYMLFF 430 440 450 LLEWLYPRDE IDLAPKWPKK TVSNETKEAR ET

References

[1]	"Comparative genome analysis of a Saccharomyces cerevisiae wine strain." Borneman A.R., Forgan A.H., Pretorius I.S., Chambers P.J. FEMS Yeast Res. 8:1185-1195(2008) [PubMed: 18778279] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	ABSV01002311 Genomic DNA. Translation: EDZ68944.1. Sequence problems.
3D structure databases
ModBase	Search...
Family and domain databases
InterPro	IPR001296. Glyco_trans_1. IPR013234. PIGA_GPI_anchor_biosynthesis. [Graphical view]
Pfam	PF00534. Glycos_transf_1. 1 hit. PF08288. PIGA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GPI3_YEAS6

Accession

Primary (citable) accession number: B5VSZ6

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 16, 2009
Last sequence update:	June 16, 2009
Last modified:	October 13, 2009
This is version 7 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)