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B5RUV1

- LIPA_DEBHA

UniProt

B5RUV1 - LIPA_DEBHA

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Protein
Lipoyl synthase, mitochondrial
Gene
DEHA2G18854g
Organism
Debaryomyces hansenii (strain ATCC 36239 / CBS 767 / JCM 1990 / NBRC 0083 / IGC 2968) (Yeast) (Torulaspora hansenii)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives By similarity.UniRule annotation

Catalytic activityi

Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi112 – 1121Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi117 – 1171Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi123 – 1231Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi142 – 1421Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi146 – 1461Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi149 – 1491Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. lipoate synthase activity Source: UniProtKB-HAMAP
  3. metal ion binding Source: UniProtKB-KW
Complete GO annotation...

GO - Biological processi

  1. protein lipoylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00538; UER00593.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoyl synthase, mitochondrial (EC:2.8.1.8)
Alternative name(s):
Lipoate synthase
Short name:
LS
Short name:
Lip-syn
Lipoic acid synthase
Gene namesi
Ordered Locus Names:DEHA2G18854g
OrganismiDebaryomyces hansenii (strain ATCC 36239 / CBS 767 / JCM 1990 / NBRC 0083 / IGC 2968) (Yeast) (Torulaspora hansenii)
Taxonomic identifieri284592 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesDebaryomycetaceaeDebaryomyces
ProteomesiUP000000599: Chromosome G

Subcellular locationi

Mitochondrion Reviewed prediction UniRule annotation

GO - Cellular componenti

  1. mitochondrion Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 1414Mitochondrion Reviewed prediction
Add
BLAST
Chaini15 – 395381Lipoyl synthase, mitochondrialUniRule annotation
PRO_0000398266Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi4959.B5RUV1.

Structurei

3D structure databases

ProteinModelPortaliB5RUV1.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0320.
HOGENOMiHOG000235998.
KOiK03644.
OMAiHPHIPTK.
OrthoDBiEOG79KPR7.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00206. Lipoyl_synth.
InterProiIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view]
PANTHERiPTHR10949. PTHR10949. 1 hit.
PfamiPF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00510. lipA. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

B5RUV1-1 [UniParc]FASTAAdd to Basket

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MISLRSISRS PAVQPARIYR TLATADTSGI NEQAKPKTKR KKTVFSDSLN    50
KGPSFEDFVN GKAADMLVDP LEAARQDPNQ RLPKWLKVPI PKGKSFNNLK 100
NDVRELKLAT VCEEAKCPNI GECWGGKKSE ATATIMLMGD TCTRGCRFCS 150
VKTSRAPAKP DPMEPENTAE AISRWGLGYV VLTTVDRDDL VDGGANHLAE 200
TVRKIKEKAP QILVEVLGGD FRGDLDMAAI LARSGLDVYA HNIETVEALT 250
PYVRDRRATY RQSLSILNKA KETKPSLVTK TSLMLGFGET DEQIMQTLKD 300
LREIKCDVVT FGQYMRPTKR HMKVVDYVKP EKFDYWRDTA LQMGFLYVAS 350
GPLVRSSYKA GEAFIENVIR KRRHNVGETP RLAQEVNPKI ISQSI 395
Length:395
Mass (Da):44,126
Last modified:November 4, 2008 - v1
Checksum:i0E99DCBC39A30A45
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CR382139 Genomic DNA. Translation: CAR65995.1.
RefSeqiXP_002770663.1. XM_002770617.1.

Genome annotation databases

GeneIDi8999246.
KEGGidha:DEHA2G18854g.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CR382139 Genomic DNA. Translation: CAR65995.1 .
RefSeqi XP_002770663.1. XM_002770617.1.

3D structure databases

ProteinModelPortali B5RUV1.
ModBasei Search...

Protein-protein interaction databases

STRINGi 4959.B5RUV1.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 8999246.
KEGGi dha:DEHA2G18854g.

Phylogenomic databases

eggNOGi COG0320.
HOGENOMi HOG000235998.
KOi K03644.
OMAi HPHIPTK.
OrthoDBi EOG79KPR7.

Enzyme and pathway databases

UniPathwayi UPA00538 ; UER00593 .

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_00206. Lipoyl_synth.
InterProi IPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view ]
PANTHERi PTHR10949. PTHR10949. 1 hit.
Pfami PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF005963. Lipoyl_synth. 1 hit.
SMARTi SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00510. lipA. 1 hit.
ProtoNeti Search...

Entry informationi

Entry nameiLIPA_DEBHA
AccessioniPrimary (citable) accession number: B5RUV1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 5, 2010
Last sequence update: November 4, 2008
Last modified: June 11, 2014
This is version 41 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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