B5RRS4 (LSPA_BORRA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Lipoprotein signal peptidase
EC=3.4.23.36

Alternative name(s):
Prolipoprotein signal peptidase
Signal peptidase II
Short name=SPase II

Gene names

Name:	lspA
Ordered Locus Names:	BRE_476

Organism

Borrelia recurrentis (strain A1) [Complete proteome] [HAMAP]

Taxonomic identifier

412418 [NCBI]

Taxonomic lineage

Bacteria › Spirochaetes › Spirochaetales › Spirochaetaceae › Borrelia

Protein attributes

Sequence length	170 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	This protein specifically catalyzes the removal of signal peptides from prolipoproteins By similarity. HAMAP MF_00161
Catalytic activity	Release of signal peptides from bacterial membrane prolipoproteins. Hydrolyzes -Xaa-Yaa-Zaa-|-(S,diacylglyceryl)Cys-, in which Xaa is hydrophobic (preferably Leu), and Yaa (Ala or Ser) and Zaa (Gly or Ala) have small, neutral side chains. HAMAP MF_00161
Pathway	Protein modification; lipoprotein biosynthesis (signal peptide cleavage). HAMAP MF_00161
Subcellular location	Cell inner membrane; Multi-pass membrane protein By similarity HAMAP MF_00161.
Sequence similarities	Belongs to the peptidase A8 family.

Ontologies

Keywords
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Aspartyl protease Hydrolase Protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	aspartic-type endopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 170	170	Lipoprotein signal peptidase HAMAP MF_00161		PRO_1000097233
Regions
	Transmembrane	13 – 33	21	Helical; Potential
	Transmembrane	72 – 92	21	Helical; Potential
	Transmembrane	96 – 113	18	Helical; Potential
	Transmembrane	116 – 136	21	Helical; Potential
	Transmembrane	142 – 162	21	Helical; Potential
Sites
	Active site	114	1	By similarity
	Active site	146	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	B5RRS4 [UniParc]. Last modified November 4, 2008. Version 1. Checksum: DFC25BE96F6ACACD	FASTA	170	19,589
	10 20 30 40 50 60 MNINRNRLVS NLIFISILVF FDQWSKYLVV TYVRLGTEYL SFFGDLFKII HVRNTGVLFS 70 80 90 100 110 120 LGSNIDSSLK NLFFLIIPII ILVFVFSFSL KENNKVSRFA LILILSGGIG NIIDRLFRPL 130 140 150 160 170 GVVDFLDVKF FGIFGLQRWP TFNFADSYVV VGMIVFIIYD LFTKDKSTNL

References

[1]

"The genome of Borrelia recurrentis, the agent of deadly louse-borne relapsing fever, is a degraded subset of tick-borne Borrelia duttonii." Lescot M., Audic S., Robert C., Nguyen T.T., Blanc G., Cutler S.J., Wincker P., Couloux A., Claverie J.-M., Raoult D., Drancourt M. PLoS Genet. 4:E1000185-E1000185(2008) [PubMed: 18787695] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: A1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000993 Genomic DNA. Translation: ACH94708.1.
RefSeq	YP_002222929.1. NC_011244.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	B5RRS4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBORT00000005358; EBBORP00000004933; EBBORG00000005358.
GeneID	6919018.
GenomeReviews	Gene locus BRE_476 in contig CP000993_GR.
KEGG	bre:BRE_476.
PATRIC	20570115. VBIBorRec40566_0495.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000007459.
HOGENOM	HBG724422.
OMA	ITLFIIY.
ProtClustDB	PRK01574.
Family and domain databases
HAMAP	MF_00161. LspA. [Tree]
InterPro	IPR001872. Peptidase_A8. [Graphical view]
KO	K03101.
Pfam	PF01252. Peptidase_A8. 1 hit. [Graphical view]
PRINTS	PR00781. LIPOSIGPTASE.
TIGRFAMs	TIGR00077. LspA. 1 hit.
PROSITE	PS00855. SPASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LSPA_BORRA

Accession

Primary (citable) accession number: B5RRS4

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	November 4, 2008
Last modified:	January 25, 2012
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program