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B5RR67 (SYL_BORRA) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 44. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:BRE_252
OrganismBorrelia recurrentis (strain A1) [Complete proteome] [HAMAP]
Taxonomic identifier412418 [NCBI]
Taxonomic lineageBacteriaSpirochaetesSpirochaetalesSpirochaetaceaeBorrelia

Protein attributes

Sequence length842 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 842842Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000091294

Regions

Motif44 – 5512"HIGH" region HAMAP-Rule MF_00049
Motif619 – 6235"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6221ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
B5RR67 [UniParc].

Last modified November 4, 2008. Version 1.
Checksum: 1636331275CE01F9

FASTA84298,708
        10         20         30         40         50         60 
MSKYDFKKIE KKWQNYWDKH KTYKVNEDPN VPKEKRIYIL DMFPYPSANG LHVGHPEGYT 

        70         80         90        100        110        120 
ATDILTRYKL LNGFNVLHPM GFDSFGLPAE NYAIQTGKHP KKITEKNIEK FKEQIKALGF 

       130        140        150        160        170        180 
AYDWDREIKT HDVNYYKWTQ WIFLQLYKKG LAYTKEIPVW YCPDLGTVLA NEEVIQTPDG 

       190        200        210        220        230        240 
PRSERGFHKV ERKPLRQWLL KITKYAERLI RDLEEVDWPD SVKEMQKNWI GKSTGVEIEF 

       250        260        270        280        290        300 
LIKESKEKIK VFTTRPDTIF GVTYLVLAPE HPMVDKITKD ELKPIISKYK DKEILKSDLE 

       310        320        330        340        350        360 
RTSLEKDKTG IFTGAYAINP ITKEEIPIWI GSYILGTYGT GAVMSVPAHD ERDFEFAKKY 

       370        380        390        400        410        420 
NLPIKQVVSQ TGTNEVLIKP FTENGISINT PTEFNNLKTI EVKTKVIKWL IENKMGQEKV 

       430        440        450        460        470        480 
NYKLRDWIFS RQRYWGEPIP ILFDDNLNEI PLNDDELPLT LPDIENYKPS GTGESPLSKI 

       490        500        510        520        530        540 
KDWVNVKRNG KIYKRETNTM PQWAGSCWYY IRYLDPHNEK EFANKEKINY WMPVDLYIGG 

       550        560        570        580        590        600 
AEHSVLHLLY ARFWHKVLYD LGYVNIKEPF RKLINQGMIT SFAYQDENGI LIPNDEVEKK 

       610        620        630        640        650        660 
DNKFFSKKNN KELKQIIAKM SKSLKNIINP DDIIKEYGAD SMRIYEMFMG PLTDSKPWNT 

       670        680        690        700        710        720 
QGLIGIFRFL NKIWLIKNKE LTNETPPKEI ISELHKTIKK VTEDIETLNF NTAISTLMIF 

       730        740        750        760        770        780 
INELLKHEKN YLKIFRPISI ILSPFAPHLG EELWEFMGEQ SSIFKNAKWP KYDLNSIIDD 

       790        800        810        820        830        840 
TREIVLQVNG KTKDKIMIKK DTDEKTLKKI AFNNQKIIQN INNKQIIKII TVKDKLVNIV 


AK 

« Hide

References

[1]"The genome of Borrelia recurrentis, the agent of deadly louse-borne relapsing fever, is a degraded subset of tick-borne Borrelia duttonii."
Lescot M., Audic S., Robert C., Nguyen T.T., Blanc G., Cutler S.J., Wincker P., Couloux A., Claverie J.-M., Raoult D., Drancourt M.
PLoS Genet. 4:E1000185-E1000185(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: A1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000993 Genomic DNA. Translation: ACH94501.1.
RefSeqYP_002222722.1. NC_011244.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING412418.BRE_252.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACH94501; ACH94501; BRE_252.
GeneID6918794.
KEGGbre:BRE_252.
PATRIC20569649. VBIBorRec40566_0272.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200748.
KOK01869.
OMAVNIVTND.
OrthoDBEOG63Z74X.

Enzyme and pathway databases

BioCycBREC412418:GJIA-252-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_BORRA
AccessionPrimary (citable) accession number: B5RR67
Entry history
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: November 4, 2008
Last modified: May 14, 2014
This is version 44 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries