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Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Borrelia recurrentis (strain A1)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei622 – 6221ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. leucine-tRNA ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. leucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBREC412418:GJIA-252-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Short name:
LeuRSUniRule annotation
Gene namesi
Name:leuSUniRule annotation
Ordered Locus Names:BRE_252
OrganismiBorrelia recurrentis (strain A1)
Taxonomic identifieri412418 [NCBI]
Taxonomic lineageiBacteriaSpirochaetesSpirochaetalesSpirochaetaceaeBorrelia
ProteomesiUP000000612: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 842842Leucine--tRNA ligasePRO_1000091294Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi412418.BRE_252.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi44 – 5512"HIGH" regionAdd
BLAST
Motifi619 – 6235"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200748.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B5RR67-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKYDFKKIE KKWQNYWDKH KTYKVNEDPN VPKEKRIYIL DMFPYPSANG
60 70 80 90 100
LHVGHPEGYT ATDILTRYKL LNGFNVLHPM GFDSFGLPAE NYAIQTGKHP
110 120 130 140 150
KKITEKNIEK FKEQIKALGF AYDWDREIKT HDVNYYKWTQ WIFLQLYKKG
160 170 180 190 200
LAYTKEIPVW YCPDLGTVLA NEEVIQTPDG PRSERGFHKV ERKPLRQWLL
210 220 230 240 250
KITKYAERLI RDLEEVDWPD SVKEMQKNWI GKSTGVEIEF LIKESKEKIK
260 270 280 290 300
VFTTRPDTIF GVTYLVLAPE HPMVDKITKD ELKPIISKYK DKEILKSDLE
310 320 330 340 350
RTSLEKDKTG IFTGAYAINP ITKEEIPIWI GSYILGTYGT GAVMSVPAHD
360 370 380 390 400
ERDFEFAKKY NLPIKQVVSQ TGTNEVLIKP FTENGISINT PTEFNNLKTI
410 420 430 440 450
EVKTKVIKWL IENKMGQEKV NYKLRDWIFS RQRYWGEPIP ILFDDNLNEI
460 470 480 490 500
PLNDDELPLT LPDIENYKPS GTGESPLSKI KDWVNVKRNG KIYKRETNTM
510 520 530 540 550
PQWAGSCWYY IRYLDPHNEK EFANKEKINY WMPVDLYIGG AEHSVLHLLY
560 570 580 590 600
ARFWHKVLYD LGYVNIKEPF RKLINQGMIT SFAYQDENGI LIPNDEVEKK
610 620 630 640 650
DNKFFSKKNN KELKQIIAKM SKSLKNIINP DDIIKEYGAD SMRIYEMFMG
660 670 680 690 700
PLTDSKPWNT QGLIGIFRFL NKIWLIKNKE LTNETPPKEI ISELHKTIKK
710 720 730 740 750
VTEDIETLNF NTAISTLMIF INELLKHEKN YLKIFRPISI ILSPFAPHLG
760 770 780 790 800
EELWEFMGEQ SSIFKNAKWP KYDLNSIIDD TREIVLQVNG KTKDKIMIKK
810 820 830 840
DTDEKTLKKI AFNNQKIIQN INNKQIIKII TVKDKLVNIV AK
Length:842
Mass (Da):98,708
Last modified:November 4, 2008 - v1
Checksum:i1636331275CE01F9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000993 Genomic DNA. Translation: ACH94501.1.
RefSeqiYP_002222722.1. NC_011244.1.

Genome annotation databases

EnsemblBacteriaiACH94501; ACH94501; BRE_252.
GeneIDi6918794.
KEGGibre:BRE_252.
PATRICi20569649. VBIBorRec40566_0272.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000993 Genomic DNA. Translation: ACH94501.1.
RefSeqiYP_002222722.1. NC_011244.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi412418.BRE_252.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACH94501; ACH94501; BRE_252.
GeneIDi6918794.
KEGGibre:BRE_252.
PATRICi20569649. VBIBorRec40566_0272.

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200748.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Enzyme and pathway databases

BioCyciBREC412418:GJIA-252-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The genome of Borrelia recurrentis, the agent of deadly louse-borne relapsing fever, is a degraded subset of tick-borne Borrelia duttonii."
    Lescot M., Audic S., Robert C., Nguyen T.T., Blanc G., Cutler S.J., Wincker P., Couloux A., Claverie J.-M., Raoult D., Drancourt M.
    PLoS Genet. 4:E1000185-E1000185(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: A1.

Entry informationi

Entry nameiSYL_BORRA
AccessioniPrimary (citable) accession number: B5RR67
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: November 4, 2008
Last modified: March 4, 2015
This is version 50 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.