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B5RQH0

- SYI_BORRA

UniProt

B5RQH0 - SYI_BORRA

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Borrelia recurrentis (strain A1)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei597 – 5971ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciBREC412418:GJIA-844-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:BRE_844
OrganismiBorrelia recurrentis (strain A1)
Taxonomic identifieri412418 [NCBI]
Taxonomic lineageiBacteriaSpirochaetesSpirochaetalesSpirochaetaceaeBorrelia
ProteomesiUP000000612: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10441044Isoleucine--tRNA ligasePRO_1000216251Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi412418.BRE_844.

Structurei

3D structure databases

ProteinModelPortaliB5RQH0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi48 – 5811"HIGH" regionAdd
BLAST
Motifi594 – 5985"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246403.
KOiK01870.
OMAiKWIISEI.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.

Sequencei

Sequence statusi: Complete.

B5RQH0-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MFKKVENKVH FPQLEEKILQ FWNHNKIFEK SMKQREGCEE FTFYDGPPFA
60 70 80 90 100
TGLPHFGHFV PNTIKDIIPR YQTMKGKNVK RYFGWDTHGL PVEYEVEKSL
110 120 130 140 150
KLSGRYEIEQ YGIDKFNEEC RNIVLRYTKE WKKIITRLGR WVDFENNYKT
160 170 180 190 200
MDLTFMESVW WVFKTLYNKG LIYESYYVLP YSPKLATPLS NFEVNLGEYK
210 220 230 240 250
EIHDPSLTIK FKIKDKNEYL LAWTTTPWTL PTNLGIAVGK DIDYSKVVDQ
260 270 280 290 300
EKNEIYIIGT KRLNHYYQDE NKYVIIEQFK GEHLKGIEYE PLFDYFVNQR
310 320 330 340 350
NKGAFKIHTA EYVTTDDGTG IVHIAPFGEE DYQILKKNTQ TDMITPIDAE
360 370 380 390 400
CKFTSEVKDF EGLFVKDADN KIIEKLKSMN LLFKRENYLH RYPFCYRTNS
410 420 430 440 450
PLIYRPISSW FVNIEKIKEK LIRSNEQINW IPEHLKKGRF GKWLENARDW
460 470 480 490 500
AISRNRFWGN PIPIWKCSKT GNKICIGSRE ELEKLSGQKI IDLHKDKIDK
510 520 530 540 550
ITWPSKYGGT YVRTSEVLDC WFESGSMPYA SKHYPFKDKD KFQNIFPADF
560 570 580 590 600
IAEGLDQTRG WFYTLTILGT ALFEKTAFKN VIVNGLVLSS DGKKMSKSLK
610 620 630 640 650
NYTDPIQIIN TFGADALRLY LIMSPVIKAD DLKYSDDGVK DVLKNIIIPI
660 670 680 690 700
WNAYSFFITY AIIDKFTPNN YVNLYKTNIL DKWIISEIES LKQILNEEID
710 720 730 740 750
KYNLTKSIEV LLTFIDKLNN WYIRRSRRRF WKSENDNDKI DAYETLYYTL
760 770 780 790 800
KNLMLMLAPF IPFLTEEIYQ NLKTKNEKES IHLNNYPQSI KELINIELEE
810 820 830 840 850
KMNFTRKVIT IARALRASHN IKIRKPIKTI YIITKNHKEQ NTLREMTEII
860 870 880 890 900
LEEINAKEIK IKSNEEELVT YKAKANFKEL GSKLGTNMKS VALAITKLSN
910 920 930 940 950
EDILEIINGN KHTITINNNT YDITLKDIIL ERHERKNLKV INEDSITIGL
960 970 980 990 1000
DTLITEELYL EGLSRELIRK VQNLRKESNF NVTDRIILYT NNDEILTKII
1010 1020 1030 1040
NNFENYIKTE TLAITIEINN KKALTTLELD EEISVNIGIE KCLN
Length:1,044
Mass (Da):122,975
Last modified:November 4, 2008 - v1
Checksum:iC333F3692BF3F771
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000993 Genomic DNA. Translation: ACH95054.1.
RefSeqiWP_012539206.1. NC_011244.1.
YP_002223275.1. NC_011244.1.

Genome annotation databases

EnsemblBacteriaiACH95054; ACH95054; BRE_844.
GeneIDi6919469.
KEGGibre:BRE_844.
PATRICi20570870. VBIBorRec40566_0853.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000993 Genomic DNA. Translation: ACH95054.1 .
RefSeqi WP_012539206.1. NC_011244.1.
YP_002223275.1. NC_011244.1.

3D structure databases

ProteinModelPortali B5RQH0.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 412418.BRE_844.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACH95054 ; ACH95054 ; BRE_844 .
GeneIDi 6919469.
KEGGi bre:BRE_844.
PATRICi 20570870. VBIBorRec40566_0853.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246403.
KOi K01870.
OMAi KWIISEI.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci BREC412418:GJIA-844-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02003. Ile_tRNA_synth_type2.
InterProi IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "The genome of Borrelia recurrentis, the agent of deadly louse-borne relapsing fever, is a degraded subset of tick-borne Borrelia duttonii."
    Lescot M., Audic S., Robert C., Nguyen T.T., Blanc G., Cutler S.J., Wincker P., Couloux A., Claverie J.-M., Raoult D., Drancourt M.
    PLoS Genet. 4:E1000185-E1000185(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: A1.

Entry informationi

Entry nameiSYI_BORRA
AccessioniPrimary (citable) accession number: B5RQH0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: November 4, 2008
Last modified: November 26, 2014
This is version 50 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3