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B5RQH0

- SYI_BORRA

UniProt

B5RQH0 - SYI_BORRA

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Protein
Isoleucine--tRNA ligase
Gene
ileS, BRE_844
Organism
Borrelia recurrentis (strain A1)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zinc By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei597 – 5971ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. aminoacyl-tRNA editing activity Source: InterPro
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP
Complete GO annotation...

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciBREC412418:GJIA-844-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:BRE_844
OrganismiBorrelia recurrentis (strain A1)
Taxonomic identifieri412418 [NCBI]
Taxonomic lineageiBacteriaSpirochaetesSpirochaetalesSpirochaetaceaeBorrelia
ProteomesiUP000000612: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10441044Isoleucine--tRNA ligaseUniRule annotation
PRO_1000216251Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi412418.BRE_844.

Structurei

3D structure databases

ProteinModelPortaliB5RQH0.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi48 – 5811"HIGH" regionUniRule annotation
Add
BLAST
Motifi594 – 5985"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246403.
KOiK01870.
OMAiKWIISEI.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.

Sequencei

Sequence statusi: Complete.

B5RQH0-1 [UniParc]FASTAAdd to Basket

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MFKKVENKVH FPQLEEKILQ FWNHNKIFEK SMKQREGCEE FTFYDGPPFA     50
TGLPHFGHFV PNTIKDIIPR YQTMKGKNVK RYFGWDTHGL PVEYEVEKSL 100
KLSGRYEIEQ YGIDKFNEEC RNIVLRYTKE WKKIITRLGR WVDFENNYKT 150
MDLTFMESVW WVFKTLYNKG LIYESYYVLP YSPKLATPLS NFEVNLGEYK 200
EIHDPSLTIK FKIKDKNEYL LAWTTTPWTL PTNLGIAVGK DIDYSKVVDQ 250
EKNEIYIIGT KRLNHYYQDE NKYVIIEQFK GEHLKGIEYE PLFDYFVNQR 300
NKGAFKIHTA EYVTTDDGTG IVHIAPFGEE DYQILKKNTQ TDMITPIDAE 350
CKFTSEVKDF EGLFVKDADN KIIEKLKSMN LLFKRENYLH RYPFCYRTNS 400
PLIYRPISSW FVNIEKIKEK LIRSNEQINW IPEHLKKGRF GKWLENARDW 450
AISRNRFWGN PIPIWKCSKT GNKICIGSRE ELEKLSGQKI IDLHKDKIDK 500
ITWPSKYGGT YVRTSEVLDC WFESGSMPYA SKHYPFKDKD KFQNIFPADF 550
IAEGLDQTRG WFYTLTILGT ALFEKTAFKN VIVNGLVLSS DGKKMSKSLK 600
NYTDPIQIIN TFGADALRLY LIMSPVIKAD DLKYSDDGVK DVLKNIIIPI 650
WNAYSFFITY AIIDKFTPNN YVNLYKTNIL DKWIISEIES LKQILNEEID 700
KYNLTKSIEV LLTFIDKLNN WYIRRSRRRF WKSENDNDKI DAYETLYYTL 750
KNLMLMLAPF IPFLTEEIYQ NLKTKNEKES IHLNNYPQSI KELINIELEE 800
KMNFTRKVIT IARALRASHN IKIRKPIKTI YIITKNHKEQ NTLREMTEII 850
LEEINAKEIK IKSNEEELVT YKAKANFKEL GSKLGTNMKS VALAITKLSN 900
EDILEIINGN KHTITINNNT YDITLKDIIL ERHERKNLKV INEDSITIGL 950
DTLITEELYL EGLSRELIRK VQNLRKESNF NVTDRIILYT NNDEILTKII 1000
NNFENYIKTE TLAITIEINN KKALTTLELD EEISVNIGIE KCLN 1044
Length:1,044
Mass (Da):122,975
Last modified:November 4, 2008 - v1
Checksum:iC333F3692BF3F771
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000993 Genomic DNA. Translation: ACH95054.1.
RefSeqiYP_002223275.1. NC_011244.1.

Genome annotation databases

EnsemblBacteriaiACH95054; ACH95054; BRE_844.
GeneIDi6919469.
KEGGibre:BRE_844.
PATRICi20570870. VBIBorRec40566_0853.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000993 Genomic DNA. Translation: ACH95054.1 .
RefSeqi YP_002223275.1. NC_011244.1.

3D structure databases

ProteinModelPortali B5RQH0.
ModBasei Search...

Protein-protein interaction databases

STRINGi 412418.BRE_844.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACH95054 ; ACH95054 ; BRE_844 .
GeneIDi 6919469.
KEGGi bre:BRE_844.
PATRICi 20570870. VBIBorRec40566_0853.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246403.
KOi K01870.
OMAi KWIISEI.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci BREC412418:GJIA-844-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02003. Ile_tRNA_synth_type2.
InterProi IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "The genome of Borrelia recurrentis, the agent of deadly louse-borne relapsing fever, is a degraded subset of tick-borne Borrelia duttonii."
    Lescot M., Audic S., Robert C., Nguyen T.T., Blanc G., Cutler S.J., Wincker P., Couloux A., Claverie J.-M., Raoult D., Drancourt M.
    PLoS Genet. 4:E1000185-E1000185(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: A1.

Entry informationi

Entry nameiSYI_BORRA
AccessioniPrimary (citable) accession number: B5RQH0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: November 4, 2008
Last modified: May 14, 2014
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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